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pTK81_GFP-4.1R

Plasmid

#46352

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1R (EPB41 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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IMS-HyPer2

Plasmid

#60248

Purpose

Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

IMS-HyPer2

Tags

Mitochondrial intermembrane space targeting signa…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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H2B-mNeonGreen-IRESpuro2

Plasmid

#183745

Purpose

Expresses H2B-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

H2B (H2BC16P Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_502_sgCD45

Plasmid

#158705

Purpose

CD45 CRISPRa control

Depositor

John Doench, David Root

Article

Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

Insert

CD45 (PTPRC Human)

Use

Lentiviral

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-RAB10 T23N

Plasmid

#130885

Purpose

Expresses human RAB10 T23N mutant with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

T23N

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKL003

Plasmid

#98919

Purpose

Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

PROPS

Tags

His

Expression

Bacterial

Promoter

BBa_J23118 - Strong Constitutive

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pKM263-ScPPX

Plasmid

#38327

Depositor

Florian Freimoser

Article

Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.

Insert

PPX1 (PPX1 Budding Yeast)

Tags

GST, His, and TEV

Expression

Bacterial

Promoter

T7

Available Since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLPCXU67SKTP

Plasmid

#27662

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

U67SKTP (RN7SK Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 20, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130997

Purpose

Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

Ef1a

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

Plasmid

#221233

Purpose

Express sgRNA targeting HEK3 loci with nCas9(D10A)

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-P2A-EGFP

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ EB3-mKate2

Plasmid

#105940

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Tags

mKate2

Expression

Bacterial

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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tol2-CAG::ChR2-YFP

Plasmid

#59740

Purpose

Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

Depositor

Ivo Lieberam

Article

Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

Insert

Channelrhodopsin-2

Tags

YFP

Expression

Mammalian

Mutation

H134R

Promoter

CAG

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
5HT6-CFP-Venus(H148G)

Plasmid

#47501

Purpose

Fluorescent reporter for pH inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

Venus(H148G)

Tags

5HT6 and CFP

Expression

Mammalian

Mutation

H148G mutation

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
TDP-43 mRuby

Plasmid

#216225

Purpose

TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

Depositor

Jennifer Lee

Article

Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

Insert

TDP-43 (TARDBP Human)

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMU-AUTr

Plasmid

#61204

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-PAK2 wt

Plasmid

#31671

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Tags

GST

Expression

Bacterial

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pL-RPB1

Plasmid

#10981

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type RPB1 gene

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-T17N

Plasmid

#22018

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutation T17N.

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.3-3XFLAG-AGO2

Plasmid

#136687

Purpose

Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

Depositor

David Bartel

Article

McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

Insert

argonaute RISC Catalytic Component 2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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