We narrowed to 10,318 results for: plasmids 101

1. pLY088_AAV_EFS-BCMA-41BBz-PRODH2

   Plasmid

   #192191

   Purpose

   BCMA CAR AAV vector PRODH2 KI (pLY088)

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   BCMA CAR AAV vector PRODH2 KI (pLY088) (TNFRSF17 Synthetic)
   

   Use

   AAV; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS D614G soluble Spike

   Plasmid

   #164651

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CAG

   Available Since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

3. pGL3-noSV40-humanEC1.25_S3-4-5-6

   Plasmid

   #173956

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Enhancer regions 3-6 (S3, S4, S5 and S6) from SOX9 enhancer cluster EC1.25
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTwist-SARS-CoV-2 Spike-deltaC18 BA.4 (V3G,S658N)

   Plasmid

   #212504

   Purpose

   Encodes SARS-CoV-2 variant BA.4 Spike V3G,S658N for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.4 (contains third aa mutation and without N658S) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIG-806_HA-GD2-28z_CAR_RFP-TFAP4

   Plasmid

   #207493

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIG-851_HA-GD2-28z_HA-TFAP4

   Plasmid

   #207501

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   HA-TFAP4, HA-GD2-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGK-HA-PGRN

   Plasmid

   #234872

   Purpose

   Lentiviral plasmid expressing HA-tagged human progranulin.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   Progranulin (GRN Human)
   

   Use

   Lentiviral

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-optimized E6AP F727D

   Plasmid

   #165101

   Purpose

   ecoli optimized expression of a GST fusion of Human E6AP F727D

   Depositor

   Arthur Haas

   Article

   Ronchi et al J Biol Chem. 2014 Jan 10;289(2):1033-48.

   Insert

   E6AP F727D (UBE3A Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. gh107

   Plasmid

   #106777

   Purpose

   expression of gRNA targeting B3GALT4

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   B3GALT4 (B3GALT4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. gh82

    Plasmid

    #106753

    Purpose

    expression of gRNA targeting MGAT4D

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    MGAT4D (LOC152586 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

    Plasmid

    #228902

    Purpose

    For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

    Depositor

    Bryan Roth

    Article

    Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

    Insert

    hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
    

    Use

    AAV and Synthetic Biology

    Tags

    HA and P2A mCitrine

    Expression

    Mammalian

    Mutation

    R108K

    Promoter

    Chicken Beta Actin

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV:ITR-U6-sgRNA(backbone)-pCBh-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60229

    Purpose

    Expresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET11a_3xFlag-nsp14 (SARS-CoV-2)

    Plasmid

    #169159

    Purpose

    To express SARS-CoV-2 nsp14 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CMV-FLEX-SaCas9-U6-sgCnr1

    Plasmid

    #209196

    Purpose

    Mutagenesis of Cnr1

    Depositor

    Larry Zweifel

    Article

    Gunduz-Cinar et al Neuron. 2023 Jul 19:S0896-6273(23)00482-

    Insert

    Cnr1 (Cnr1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPaint-mScarlet-PuroR [M1G]

    Plasmid

    #171809

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS SARS-CoV-2 D614G Spike

    Plasmid

    #185692

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 D614G Spike

    Depositor

    Marceline Côté

    Article

    Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

    Insert

    SARS-CoV-2 D614G Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutation: D614G

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available Since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCMV-T7-ABE8e-SpCas9-NRCH-P2A-EGFP (RMD57)

    Plasmid

    #197504

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRCH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-ABE8e-NRCH-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRCH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-Htt171-66Q-myc-WPRE

    Plasmid

    #107934

    Purpose

    Adeno-Associated viral (AAV) vector plasmid expressing exon1 of mutant HTT (mHTT, 66 polyQ repeats) in neurons

    Depositor

    Johan Jakobsson

    Article

    Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

    Insert

    Exon 1 of mutant HTT, 66 polyQ repeats (HTT Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPaint-mScarlet-BlastR [M1G]

    Plasmid

    #171814

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-Htt171-18Q-myc-WPRE

    Plasmid

    #107936

    Purpose

    Adeno-Associated viral (AAV) vector plasmid expressing exon1 of wild type HTT (wtHTT, 18 polyQ repeats) in neurons

    Depositor

    Johan Jakobsson

    Article

    Pircs et al Cell Rep. 2018 Aug 7;24(6):1397-1406. do

    Insert

    Exon 1 of wild type HTT (HTT Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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