We narrowed to 22,946 results for: Tes

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p2T-CMV-HNHx-ABE8eHD-BlastR

Plasmid

#176478

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Article

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Insert

HNHx-ABE8eHD

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4-Hsp70

Plasmid

#62898

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70

Plasmid

#62911

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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H9 F42A pAcGP67a

Plasmid

#41810

Depositor

Chris Garcia

Article

Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

Insert

H9 Super-2 (IL2 Human)

Tags

8x His

Expression

Insect

Mutation

Phenylalanine 42 to Alanine

Promoter

Polyhedron

Available Since

Jan. 8, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/NS3

Plasmid

#120822

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS3 ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN-AS-HNF4α

Plasmid

#139305

Purpose

Knocking down of human HNF4α through shRNA and amiRNA targeting HNF4α

Depositor

Claes Wadelius

Article

Pan et al iScience. 2020 Jan 9:100808. doi: 10.101

Insert

shRNA and amiRNA against HNF4α

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR223-BILF1

Plasmid

#195797

Purpose

Gateway Entry Clone of Epstein-Barr virus BILF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BILF1 (BILF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BILF2

Plasmid

#195788

Purpose

Gateway Entry Clone of Epstein-Barr virus BILF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BILF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pPL5618_pUG_MtxR

Plasmid

#60929

Purpose

PCR template vector for generating methotrexate resistance cassette.

Depositor

Robert Piper

Article

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

Dihydrofolate reductase (DHFR Human)

Use

Loxp flanked deletion cassette template

Mutation

L22F F31S

Promoter

TEF1

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Cathepsin S

Plasmid

#84694

Purpose

for expression of Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Promoter

unknown

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-p65AD-Hsp70

Plasmid

#62915

Purpose

Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S277G

Plasmid

#13615

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic Serine 277 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pSIN-Arkadia-gRNA1

Plasmid

#180374

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/NS4B

Plasmid

#120823

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS4B ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCI syn iGluSnFR

Plasmid

#106123

Purpose

Indicator for glutamate imaging (synapsin promoter)

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR

Tags

Myc and PDGFR

Expression

Mammalian

Promoter

synapsin

Available Since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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myc-BioID-Rab7

Plasmid

#121049

Purpose

Expresses myc tagged BioID and human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Inserts

BioID
Rab7A

Tags

myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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Venus-1-155-Ggamma2

Plasmid

#234752

Purpose

Mammalian expression of BiFC Venus-betagamma (with Venus-155-239-Gbeta1)

Depositor

Nevin Lambert

Article

Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

Insert

GNG2 (GNG2 Human)

Tags

VC155

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Venus-155-239-Gbeta1

Plasmid

#234751

Purpose

Mammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)

Depositor

Nevin Lambert

Article

Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

Insert

GNB1 (GNB1 Human)

Tags

VN155

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(0)-T2A-Gal4-Hsp70)

Plasmid

#62954

Purpose

Singlet donor (phase 0) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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LNCX BMP-RII

Plasmid

#12641

Depositor

Rik Derynck

Article

Skillington et al J Cell Biol. 2002 Oct 14. 159(1):135-46.

Insert

BMP-RII (BMPR2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Oct. 17, 2006

Availability

Academic Institutions and Nonprofits only
pMXs-NKX2.2

Plasmid

#134670

Purpose

Retroviral expression of mouse Nkx2.2

Depositor

Marius Wernig

Article

Tanabe et al Development. 2022 Oct 15;149(20). pii: 2

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Retroviral

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(1)-T2A-Gal4-Hsp70)

Plasmid

#62955

Purpose

Singlet donor (phase 1) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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CaV2.1 (pCR3.1)

Plasmid

#26573

Depositor

Diane Lipscombe

Article

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Expression

Mammalian

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only
pRDB_118

Plasmid

#216089

Purpose

Cas9 [Sp] CRISPRa targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2/NS2B

Plasmid

#120819

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

NS2B ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-SRGAP1

Plasmid

#187269

Purpose

Expression of SRGAP1-EGFP

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

SRGAP1 (SRGAP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
mt deltaN20 TFB2MdeltaC3

Plasmid

#205062

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 3 aa

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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PLK1-cjun-FRET-PACT biosensor

Plasmid

#106907

Purpose

centrosome-localized PLK1 FRET-activity biosensor (through PACT domain with 10aa linker)

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

PLK1-cjun-FRET-PACT biosensor with linker (PLK1 Human)

Tags

PACT domain

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(1)-T2A-LexA::QFAD-Hsp70

Plasmid

#62948

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
L_NLS 67kDa

Plasmid

#201361

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-6PrA

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
H_NLS 67kDa

Plasmid

#201372

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP-6PrA

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-INPP4A

Plasmid

#171944

Purpose

Expresses INPP4A in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

INPP4A (INPP4A Human)

Tags

EGFP

Expression

Mammalian

Mutation

See Depositor Comments Below

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDUAL_2_araBAD

Plasmid

#155176

Purpose

see pDUAL_araBAD: exchanged origin of replication (pBR322) and introduced bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-HA-hTet1CDmu-WPRE-PolyA

Plasmid

#39455

Depositor

Hongjun Song

Article

Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

Insert

HA-hTet1CDmu (TET1 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

aa1418–2136 with mutations H1672 to Y, 1674D to A

Promoter

EF1a

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
pMW357

Plasmid

#238204

Purpose

Expresses untagged TaTasR from a PBAD promoter, followed by the native TIGR array (8 units)

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

TaTasR

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRDA_833

Plasmid

#216086

Purpose

Cas9 [Sp] knockout targeting CD47, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD47 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL

Plasmid

#161783

Purpose

Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and GFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and hPGK

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCVpuro BRIT1

Plasmid

#16205

Depositor

Shiaw-Yih Lin

Article

Rai et al Cancer Cell. 2006 Aug . 10(2):145-57.

Insert

BRIT1 (MCPH1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only
pIVT/Smarca4

Plasmid

#122146

Purpose

For making Smarca4 RNA

Depositor

Carmen Williams

Article

Miao et al Biol Reprod. 2018 Jan 9. pii: 4794936. d

Insert

Smarca4 (Smarca4 Mouse)

Use

For in vitro transcription

Promoter

T7

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMALB

Plasmid

#161784

Purpose

Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

Bidirectional minhCMV and hPGK

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM33_PDCD6IP_ALIX V-domain

Plasmid

#178483

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PDCD6IP_ALIX V-domain (PDCD6IP Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-GFAP(long)-FLEX-GCaMP7f

Plasmid

#189612

Purpose

Cre-dependent GCaMP7f under the GFAP promoter

Depositor

Martin Riccomagno

Article

Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

Insert

GCaMP7f

Use

AAV and Cre/Lox

Tags

GCaMP7f

Expression

Mammalian

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

Plasmid

#62952

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BMRF1(EA-D)

Plasmid

#195807

Purpose

Gateway Entry Clone of Epstein-Barr virus BMRF1

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BMRF1 (BMRF1 Epstein-Barr virus)

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Diffusive 54kDa

Plasmid

#201337

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-4PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Diffusive 47kDa

Plasmid

#201336

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-3PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mRIP3 GFP RHIM mut

Plasmid

#41384

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP3 (Ripk3 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

RHIM mutant

Promoter

CMV

Available Since

Dec. 4, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-HA-CUL1 delta N53

Plasmid

#19950

Depositor

Yue Xiong

Article

Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

Insert

cullin 1 delta N53 (CUL1 Human)

Tags

HA

Expression

Mammalian

Mutation

deletion of 53 residues from the NH2-terminus(de…

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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We narrowed to 22,946 results for: Tes