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pET-6xHis-GFP(-12)

Plasmid

#219420

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-12)

Tags

MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMVN

Plasmid

#223861

Purpose

4G-cloning donor for mammalian promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phN

Plasmid

#223858

Purpose

4G-cloning donor for insect promoter and terminator, N-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-GST-TEV

Plasmid

#223878

Purpose

4G-cloning donor for N-terminal His6-GST-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-GST-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-MBP-TEV

Plasmid

#223877

Purpose

4G-cloning donor for N-terminal His6-MBP-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-MBP-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-HA2-TEV

Plasmid

#223876

Purpose

4G-cloning donor for N-terminal His6-HA2-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-HA2-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Nt)_His6-FLAG3-TEV

Plasmid

#223875

Purpose

4G-cloning donor for N-terminal His6-FLAG3-TEV tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

His6-FLAG3-TEV

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_phC

Plasmid

#223859

Purpose

4G-cloning donor for insect promoter and terminator, C-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

polyhedrin promoter and SV40 polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(Ct)_3C-Venus-His8

Plasmid

#223889

Purpose

4G-cloning donor for C-terminal 3C-Venus-His8 tag

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

3C-Venus-His8

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pD(P+T)_CMVC

Plasmid

#223862

Purpose

4G-cloning donor for mammalian promoter and terminator, C-terminal tag added

Depositor

Stephan Gruber

Article

Taschner et al bioRxiv 2024.06.17.599261

Insert

CMV promoter and bGH polyA

Use

Golden-gate donor

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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nTALECD for_TUB4S351F_1397NC

Plasmid

#193382

Purpose

nTALECD (1397NC) expression vector

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Insert

For specific C-to-T base editing to introduce an amino acid mutation in the coding sequence of AtTUB4.

Expression

Plant

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-tdTomato-sg

Plasmid

#194722

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target tdTomato

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-GFP-sg

Plasmid

#194717

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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mApple_pcDNA3.1+

Plasmid

#182855

Purpose

Monomer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mApple

Expression

Mammalian

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pJ2039_N2only

Plasmid

#140682

Purpose

Control (“C”) lacking sg-3

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Control (“C”) lacking sg-3

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2040_N2only

Plasmid

#140683

Purpose

Control (“C”) lacking sg-4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

Control (“C”) lacking sg-4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pYTK094

Plasmid

#65201

Purpose

Encodes HO 5' Homology as a Type 8b part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

HO 5' Homology

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK088

Plasmid

#65195

Purpose

Encodes HO 3' Homology as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

HO 3' Homology

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK052

Plasmid

#65159

Purpose

Encodes tSSA1 as a Type 4 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

tSSA1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK054

Plasmid

#65161

Purpose

Encodes tPGK1 as a Type 4 part to be used in the Dueber YTK system

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

tPGK1

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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