4,178 results

1. Lac-I-SceI-Tet

   Plasmid

   #17655

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   lac I-SceI tet
   

   Expression

   Bacterial

   Available Since

   May 30, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pRK5 c-Fyn

   Plasmid

   #16032

   Depositor

   Filippo Giancotti

   Article

   Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

   Insert

   c-Fyn (FYN Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. mIFP-N1

   Plasmid

   #54620

   Purpose

   Localization: N1 Cloning Vector, Excitation: 683, Emission: 704

   Depositor

   Xiaokun Shu, Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Type

   Empty backbone

   Tags

   mIFP

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pET15b PKA Cat

   Plasmid

   #14921

   Depositor

   Susan Taylor

   Article

   Narayana et al Biochemistry. 1997 Apr 15. 36(15):4438-4

   Insert

   PKA catalytic subunit alpha (Prkaca Mouse)
   

   Tags

   6xHis and Thrombin cleavage site

   Expression

   Bacterial

   Available Since

   July 24, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPGKPhiC31obpA

   Plasmid

   #13795

   Purpose

   Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

   Depositor

   Philippe Soriano

   Article

   Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

   Insert

   PhiC31o
   

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Jan. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 HA 14-3-3 sigma

   Plasmid

   #11946

   Depositor

   Michael Yaffe

   Article

   Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

   Insert

   Stratifin (SFN Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-EF1a-mCherry-IRES-Flpo

   Plasmid

   #55634

   Purpose

   Expresses Flpo in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV1

   Inserts

   mCherry
   Flpo
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Ef1a and Ef1a/IRES

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-H2B-AID-EYFP

   Plasmid

   #47329

   Purpose

   for plasmid integration using the Flp-In system. AID at C-terminal

   Depositor

   Don Cleveland

   Article

   Holland et al Proc Natl Acad Sci U S A. 2012 Dec 4;109

   Insert

   histone H2B (H2BC21 Human)
   

   Tags

   AID and EYFP

   Expression

   Mammalian

   Available Since

   Aug. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-puro-RFP-ATG9A

   Plasmid

   #60609

   Purpose

   Expresses RFP-hATG9A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

   Insert

   Homo sapiens autophagy related 9A (PPARG Human)
   

   Use

   Retroviral

   Tags

   RFP

   Expression

   Mammalian

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN3-Sp1FL

    Plasmid

    #24543

    Depositor

    Guntram Suske

    Insert

    Sp1FL (SP1 Human)
    

    Expression

    Mammalian

    Available Since

    June 16, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. 1245 pCMVb p300

    Plasmid

    #10717

    Depositor

    William Sellers

    Insert

    p300 (EP300 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET15-MHL

    Plasmid

    #26092

    Purpose

    SGC Empty backbone for bacterial expression under T7 promoter. Uses infusion based cloning method.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Sept. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. Flag-HA-USP10

    Plasmid

    #22543

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP10 (USP10 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Jan. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-EGFP-C1-HsDCP1a

    Plasmid

    #25030

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

    Insert

    DCP1a (DCP1A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-HA2-DDB1

    Plasmid

    #19909

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    Damage-specific DNA binding protein 1 (DDB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    please see depositor comment below

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. Flag-HA-USP21

    Plasmid

    #22574

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP21 (USP21 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA

    Plasmid

    #61594

    Purpose

    A catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.

    Depositor

    Feng Zhang

    Article

    Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

    Insert

    hSaCas9
    

    Use

    AAV and CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)

    Plasmid

    #101178

    Purpose

    Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    hSpCas9-Cluster1(N692A/M694A/Q695A/H698A)
    

    Tags

    3x FLAG and NLS (SV40)

    Expression

    Mammalian

    Mutation

    N692A/M694A/Q695A/H698A

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1/Puro-CAG-ASAP1

    Plasmid

    #52519

    Purpose

    For mammalian expression of the fluorescent voltage sensor ASAP1

    Depositor

    Michael Lin

    Article

    St-Pierre et al Nat Neurosci. 2014 Apr 22. doi: 10.1038/

    Insert

    Accelerated Sensor of Action Potentials 1
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. RCIscript-GoldyTALEN

    Plasmid

    #38142

    Purpose

    destination vector for the Golden Gate TALEN kit, for in vitro synthesis of TALEN mRNAs

    Depositor

    Stephen Ekker, Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Use

    Mrna transcription vector

    Tags

    AcV5 and FokI homodimer

    Mutation

    Truncate at N and C terminous

    Promoter

    T3

    Available Since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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