We narrowed to 62,126 results for: cloning

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PEA1-GFP

Plasmid

#171993

Purpose

Delivers all prime editing nickase components in a single, GFP selectable plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Lysosomal-METRIQ

Plasmid

#135401

Purpose

Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

DNAse II alpha (DNASE2 Human)

Use

Lentiviral

Tags

T2A, mCherry, and sfGFP

Expression

Mammalian

Mutation

Single-nucleotide polymorphism C432T

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR_P2R-P3_R2-Turbo-mVenus-STOP-L3

Plasmid

#127354

Purpose

gateway entry vector for making C-terminal TurboID-mVenus fusion with nuclear and non-nuclear proteins

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

TurboID (BirA mutant)

Use

Gateway Cloning

Tags

GS linker, V5, and mVenus

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSGAb-spe

Plasmid

#122000

Purpose

A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Insert

none

Use

CRISPR

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

Plasmid

#112925

Purpose

Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6 promoter, gRNA-SAM expression cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFD152

Plasmid

#125546

Purpose

derivative of pFD116 with an optimized RBS for E. coli

Depositor

David Bikard

Article

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Insert

optimized RBS

Use

CRISPR

Promoter

anhydrotetracycline Ptet promoter

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_Blast

Plasmid

#125592

Purpose

Lentiviral expression of spCas9 with blasticidin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RAD50

Plasmid

#116784

Purpose

Lentiviral expression of RAD50

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RAD50 (RAD50 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLX311 Luciferase

Plasmid

#117735

Purpose

Open reading frame vector encoding Luciferase

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

firefly Luciferase

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron-ST

Plasmid

#119034

Purpose

Express soma-localized Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron-ST

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCasKP-hph

Plasmid

#117232

Purpose

Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pME-MLS-KillerRed

Plasmid

#115517

Purpose

Middle entry plasmid containing membrane bound KillerRed fluorophore

Depositor

Marco Morsch

Article

Formella et al Redox Biol. 2018 Aug 23;19:226-234. doi:

Insert

MLS-KillerRed

Use

Gateway Cloning

Tags

MLS

Available Since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiHer24D5-8_synNotch_Gal4VP64

Plasmid

#85425

Purpose

Lentiviral vector for expression of anti-Her2 scFv 24D5-8 Gal4VP64 synthetic Notch

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

anti-Her2_4D5-8_synNotch

Use

Lentiviral

Promoter

PGK

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only
pMSCVpuro-TagRFP657

Plasmid

#96939

Purpose

Retroviral plasmid containing TagRFP657 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Tag Red Fluorescent Protein 657

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mCerulean3

Plasmid

#90234

Purpose

Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Promoter

PGK

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAT3_WT

Plasmid

#82235

Purpose

Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAT3 (STAT3 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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bZiPro

Plasmid

#86846

Purpose

express NS2B-NS3 protease in E.coli

Depositor

Dahai Luo

Article

Zhang et al Science. 2016 Dec 8. pii: aai9309.

Inserts

NS3 1-177
NS2B45-96

Tags

a hexahistidine tag and a thrombin cleavage site

Expression

Bacterial

Mutation

NS2B cofactor region (residues 45–96) and NS3 pro…

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only