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Plasmid#164036PurposeImproved PhoCl variant with faster dissociation rateDepositorPublicationInsertPhoCl2f
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGT-Kh2
Plasmid#122587PurposeMAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)DepositorInsertbeta-lactamase/sfGFP
UseSynthetic BiologyExpressionBacterialAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneExpressionBacterialPromoterTetracycline promoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
TALED_Left-ND1-E1347A
Plasmid#183895PurposeExpresses TALED_Left-ND1-E1347A in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
10TALE_Pmin_fLuc
Plasmid#160663PurposeExpression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert10TALE_Pmin_fLuc
UseLuciferaseExpressionMammalianPromoterminimalAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-E1b:eGFP-Ds
Plasmid#102417PurposeFluorescent vector for screening enhancer elements in zebrafishDepositorInsertE1b:eGFP
ExpressionBacterialPromoterZebrafish E1b minimal promoterAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CD-MPR-GFP-RUSH
Plasmid#202797PurposeRUSH cargo: Str-KDEL_IRES_SBP-EGFP-CD-MPRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertM6PR (M6PR Human)
TagsEGFPExpressionMammalianMutationChanged 40 K (AAA) with R (AGA)PromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA
Plasmid#197031PurposeVector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthumanized denOsCas12f1-VPR
ExpressionMammalianMutationD52R / T132R / D228A / D406APromoterCBh, CMV, hU6Available sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1
Plasmid#165444PurposeExpresses ACX, rXRCC1 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAPOBEC-nCas9-rXRCC1 (Apobec1 Synthetic, Streptococcus pyogenes, Rat)
ExpressionMammalianAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)
Plasmid#154840PurposeInsertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.DepositorInserthomology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
UseCRISPR and Cre/LoxExpressionWormMutationHYGR∆ encodes aa 1-226Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
H3S10ph biosensor
Plasmid#120809PurposeFRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRETDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP
ExpressionMammalianMutationThreonine 3, Threonine 6, and Threonine 11 are mu…PromoterCMVAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-dTOMATO
Plasmid#100604PurposeDonor scafold with dTOMATO positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsdTOMATO-T2A-Puro and tagBFPAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-syn-jYCaMP1
Plasmid#135423PurposeYellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfectionDepositorInsertjYCaMP1
UseAAVExpressionMammalianPromoterhSynAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pN-PITCh-mCherry
Plasmid#127887PurposeProvides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry taggingDepositorInsertPuro-mCherry
UseCRISPRExpressionMammalianAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiCROP-PuroR-P2A-GFP-U6
Plasmid#200029PurposeLentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAsDepositorTypeEmpty backboneUseLentiviralTagsPuroR-P2A-GFPExpressionMammalianAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C3001
Plasmid#91094PurposeModule C, Promoter: 35S, Gene: GFP (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP (+ BaeI site for GT donor cloning)
UseUnspecifiedPromoter35SAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMOD_A1910
Plasmid#91041PurposeModule A, Promoter: ZmUbi, Gene: TREX2-P2A-TaCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTREX2-P2A-TaCas9
UseCRISPRPromoterZmUbiAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0502
Plasmid#91012PurposeModule A, Promoter: AtUbi10, Gene: Csy4-P2A-AtCas9, Terminator: HSPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCsy4-P2A-AtCas9
UseCRISPRPromoterAtUbi10Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGTy1mhis3-AI or pGTy1his3-AI
Plasmid#62228PurposeA Ty1 element tagged with the retrotransposition indicator gene his3-AI is fused to the GAL1 promoter on a multicopy URA3-based shuttle plasmid.DepositorInsertTy1H3 retrotransposon
ExpressionYeastPromoterGAL1Available sinceFeb. 13, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
RAD Module G8-C1
Plasmid#38208DepositorInsertsSET Integrase
Bxb1 Excisonase
RESET Integrase
UseSynthetic Biology; Biobrick cloning vectorAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only