We narrowed to 6,273 results for: nls

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pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

Plasmid

#188774

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

SFFV

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-nEF-dCas9

Plasmid

#106430

Purpose

Expresses SpdCas9 from the nEF promoter in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

nEF-dCas9

Use

AAV and CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

nEF

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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HA-MDFI

Plasmid

#207836

Purpose

Tagged MDFI for western blotting and imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

MDFI (MDFI Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEvol-pAcFRS.1.t1

Plasmid

#73545

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAcFRS.1.t1
pAcFRS.1.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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PB-PEmax NG

Plasmid

#187894

Purpose

All-in-one prime editor piggyBac transposon with PEmax, NG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410)

Plasmid

#123615

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A/K34A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A/K34A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A/K34A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A/K34A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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dCas9-FOG1[N+C]

Plasmid

#100085

Purpose

dCas9 fused to two FOG1 peptide [aa 1-45] , one at the N-terminus and one at the C-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

FOG1(aa 1-45) (ZFPM1 Human)

Use

CRISPR

Tags

3XFLag-NLS-FOG1-dCas9-FOG1-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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shRNA Caspase 1 (pSicoR mCherry)

Plasmid

#53575

Purpose

shRNA against human pro-caspase 1

Depositor

Warner Greene

Article

Doitsh et al Nature. 2014 Jan 23;505(7484):509-14. do

Insert

shRNA Caspase 1 (CASP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6 for shRNA; EF1a for mCherry

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only
MBP-FUS_FL_6E

Plasmid

#98655

Purpose

Expresses MBP-tagged full length phosphomimetic FUS

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

6 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Article

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Insert

BE3(R33A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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OA-1136J

Plasmid

#153023

Purpose

Express His-MBP-TEV-CasRx recombinant protein

Depositor

Omar Akbari

Article

Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

Insert

CasRx

Tags

His-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_LC_12E

Plasmid

#98654

Purpose

Expresses MBP-tagged phosphomimetic FUS AA 1-163

Depositor

Nicolas Fawzi

Article

Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Mutation

12 S/T to E

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCW-ND-Cdt1-mCherry-Puro

Plasmid

#193766

Purpose

Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation, expressed from all-in-one pCW vector (TetOn + rtTA)

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

ND-Cdt1-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1 E179K

Plasmid

#60541

Purpose

Donor vector for human MLK1 E179K mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Mutation

E179K

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pLV-TetOn-ND-Cdt1-mCherry

Plasmid

#193761

Purpose

Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and C-terminal mCherry tag) with mutations which abroggate its S phase degradation

Depositor

Tobias Meyer

Article

Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

Insert

ND-Cdt1-mCherry (CDT1 Human)

Use

Lentiviral

Tags

mCherry

Mutation

amino acids 1-19 deleted, ΔCy mutation (aa68-70 t…

Promoter

TRE

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221 MLK1 D294A

Plasmid

#60542

Purpose

Donor vector for human MLK1 D294A mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

MLK1 (MAP3K9 Human)

Use

Gateway donor vector

Mutation

D294A

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
RP1B FUS 1-163 S12xQ

Plasmid

#127193

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 S12xQ (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

S12xQ: S30Q, S44Q, S48Q, S53Q, S70Q, S84Q, S89Q, …

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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RP1B FUS 1-163 QQ4xSS #1

Plasmid

#127194

Purpose

Protein expression for affinity purification

Depositor

Nicolas Fawzi

Article

Murthy et al Nat Struct Mol Biol. 2019 Jul;26(7):637-

Insert

FUS 1-163 QQ4xSS #1 (FUS Human)

Tags

hexahistidine

Expression

Bacterial

Mutation

QQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9

Plasmid

#136660

Purpose

Expresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNA

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Inserts

St1Cas9 LMD-9
sgRNA for St1Cas9
ABE7.10

Use

CRISPR and Synthetic Biology

Tags

SV40 NLS and hSt1Cas9

Expression

Mammalian

Promoter

CAG and U6

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJ4M/TDP-43 S48E

Plasmid

#104481

Purpose

bacterial expression of TDP-43 S48E

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Mutation

S48E

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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