We narrowed to 12,677 results for: sequence

1. WNT11-bio-His

   Plasmid

   #53430

   Purpose

   Expresses full-length Protein Wnt-11 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   WNT11 (WNT11 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19 ACMV DNA-B 1.5mer

   Plasmid

   #159135

   Purpose

   Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-B

   Depositor

   Vincent Fondong

   Article

   Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

   Insert

   ACMV DNA-B 1.5mer
   

   Use

   Infectious clone for bombarding plants

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWL502-PAM

   Plasmid

   #174382

   Purpose

   Plasmids bearing protospacer sequences with functional PAM efficiently triggered CRISPR-mediated defense in cells with corresponding spacer sequence; article demonstrates use in Haloferax mediterranei

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   PAM-spacer
   

   Use

   CRISPR; Archaeal expression

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-Sp-gRNA-IDS2_DF_A4b_attP_fwd

   Plasmid

   #182149

   Purpose

   To insert attP-fwd attachment site at IDS2 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS2-A4b-attP-fwd pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-Sp-gRNA-IDS_DF_D1b_attB_rev

   Plasmid

   #182152

   Purpose

   To insert attB-rev attachment site at IDS in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS-D1b-attB-rev pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

   Plasmid

   #182151

   Purpose

   To insert attB-rev attachment site at IDS in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS-C2c-attBrev pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd

   Plasmid

   #182150

   Purpose

   To install attP-fwd attachment site at IDS2 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS2-B7b-attP-fwd pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. UASgal-A9-Pleum

   Plasmid

   #60343

   Purpose

   Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

   Depositor

   Hal Alper

   Article

   Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

   Insert

   promoter
   

   Use

   Synthetic Biology; Yeast synthetic hybrid promote…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 6, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pFX-GalT-Venus

   Plasmid

   #174472

   Purpose

   Express Venus with golgi apparatus-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   Venus with golgi apparatus-targeting sequence
   

   Use

   Tags

   β1,4-galactose transferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCTCON2-BXG-2TAG

    Plasmid

    #158138

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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