We narrowed to 5,896 results for: dna plasmids

1. pTS2373-Tier1-PhCMV-Gal4

   Plasmid

   #169581

   Purpose

   Tier-1 vector encoding PhCMV-driven Gal4 DNA binding domain (PhCMV-Gal4-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   regulatory protein GAL4
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-XL-AncBE4max

   Plasmid

   #136254

   Purpose

   Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max

   Depositor

   Zhaohui Ye

   Article

   McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

   Insert

   AncBE4max_GFP
   

   Use

   CRISPR; Piggybac dna transposon

   Expression

   Mammalian

   Mutation

   The AncBE4max_GFP insert was PCR amplified from a…

   Promoter

   TRE3G

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. NanoLuc-TCF4(biased)-beta-Catenin-HaloTag Bidirectional Vector

   Plasmid

   #238680

   Purpose

   Express NanoLuc-TCF4(biased)-beta-Catenin-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   TCF4 (TCF4 Human)
   beta catenin (CTNNB1 Human)
   

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   biased

   Promoter

   CMV

   Available Since

   Sept. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. NanoBRET Assay Vector, NanoLuc-KRAS(G12V)

   Plasmid

   #236859

   Purpose

   Express NanoLuc(R)-KRAS(G12V) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KRAS (KRAS Human)
   

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   CMV

   Available Since

   Sept. 22, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. NanoBiT EGFR(1-673) Assay, BiBiT vector

   Plasmid

   #238756

   Purpose

   Express EGFR(1-673) Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   EGFR (EGFR Human)
   EGFR (EGFR Human)
   

   Tags

   LgBiT and SmBiT

   Expression

   Mammalian

   Mutation

   (1-673)

   Promoter

   CMV

   Available Since

   Sept. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. NanoBRET Assay Vector, BiBRET CRAF-BRAF

   Plasmid

   #238574

   Purpose

   Express CRAF-BRAF Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   CRAF (RAF1 Human)
   BRAF (BRAF Human)
   

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Sept. 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. NanoBRET Assay Vector, NanoLuc-KRAS WT

   Plasmid

   #236878

   Purpose

   Express NanoLuc(R)-KRAS WT in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KRAS (KRAS Human)
   

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   Sept. 12, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. LgBiT-CALM1/SmBiT-ADCY8 BiBiT Vector

   Plasmid

   #237114

   Purpose

   Express LgBiT-CALM1/SmBiT-ADCY8 Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   CALM1 (CALM1 Human)
   ADCY8 (ADCY8 Human)
   

   Tags

   LgBiT and SmBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. LgBiT-CYBC1:CYBB-SmBiT BiBiT Vector(CMV)

   Plasmid

   #237084

   Purpose

   Express LgBiT-CYBC1:CYBB-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Inserts

   CYBC1 (CYBC1 Human)
   CYBB (CYBB Human)
   

   Tags

   LgBiT and SmBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. NanoBRET BiBRET Vector MAVS/IFIH1

    Plasmid

    #237022

    Purpose

    Express HaloTag-MAVS/NanoLuc(R)-IFIH1 Fusion Proteins in Mammalian Cells under a CMV promoter

    Depositor

    Promega Corporation

    Has Service

    DNA

    Inserts

    MAVS (MAVS Human)
    IFIH1 (IFIH1 Human)
    

    Tags

    HaloTag (R) and NanoLuc (R)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    July 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pLKO.1 - TRC cloning vector

    Plasmid

    #10878

    Purpose

    3rd generation transfer plasmid

    Depositor

    David Root

    Article

    Moffat et al Cell. 2006 Mar 24. 124(6):1283-98.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pICE-RNaseHI-WT-NLS-mCherry

    Plasmid

    #60365

    Purpose

    Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    RNase HI
    

    Tags

    NLS from SV40 T antigen and mCherry

    Expression

    Mammalian

    Promoter

    CMV-tet

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid

    #160736

    Purpose

    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

    Plasmid

    #196703

    Purpose

    Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 D210N
    

    Expression

    Mammalian

    Mutation

    D210N, catalytically inactive

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid

    #181745

    Purpose

    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

    Depositor

    Benjamin Kleinstiver

    Article

    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

    Insert

    human/zebrafish codon optimized SpCas9
    

    Use

    In vitro transcription; t7 promoter

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-PE2

    Plasmid

    #132775

    Purpose

    Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    PE2
    

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    CMV

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

    Plasmid

    #239972

    Purpose

    Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (WT)

    Plasmid

    #196702

    Purpose

    Plasmid for transient mammalian expression of wild type RNase H1 tagged with 2xNLS and EGFP that can be used to specifically degrade the nuclear R-loops.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 wild type
    

    Expression

    Mammalian

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. SunTagng22aa

    Plasmid

    #106438

    Purpose

    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

    Depositor

    Steven Jacobsen

    Article

    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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