We narrowed to 4,881 results for: phy
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Plasmid#19966DepositorInsertD2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
UseTagsFlag and monomeric VenusExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
AEB-No-aptamer-controlmRFP1
Plasmid#63848PurposeThis pFTV1 vector contains a no-aptamer control mRNA encoding mRFP1 gene. It should be used when testing AEB-F12 and AEB-Theo45 riboswitches to measure their non-specific ligand effect on mRFP1 expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromoterJ23100Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET14b-dabAB2
Plasmid#133013PurposepET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tagDepositorInsertsdabB2
dabA2
UseTags6xHis, StrepII tag, and sfGFPExpressionBacterialMutationPromotert7Available sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPong_v2
Plasmid#47101PurposeExpresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.DepositorInsertsmPing
Pong Open Reading Frame 1 (ORF1)
Pong Transposase
UsePlant expressionTagsExpressionMutationPromoterGmUbi_AP, GmUbi_XS, and no promoterAvailable sinceOct. 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
AA331
Plasmid#215954PurposeFragmid fragment: (guide cassette) guide expressionDepositorHas ServiceCloning Grade DNAInsertU6_v1; BsmBI_v0; BsmBI_v1; trRNA_v2 [Sa]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_01
Plasmid#194662PurposeProtein production of the split HaloTag based calcium recorder Caprola_01 in bacteriaDepositorInsertCaprola_01
UseTagsHis-tag and Strep-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTol2_elavl3(HuC)_NES-Caprola_01-mEGFP
Plasmid#194696PurposeHuC driven expression of the calcium recorder Caprola_01 fused to mEGFP for neuronal expression in zebrafishDepositorInsertCaprola_01-mEGFP
UseZebrafish expressionTagsmEGFPExpressionMutationPromoterelavl3(HuC)Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-cpHalo∆
Plasmid#194659PurposeProtein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteriaDepositorInsertcpHalo∆
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-GtACR1_C102A-EYFP
Plasmid#119196PurposeStep-function mutant of Guillardia theta anion channelrhodopsin 1 (GtACR1) with dramatically prolonged open stateDepositorInsertAnion channelrhodopsin GtACR1 step-function mutant C102A
UseLentiviralTagsEYFPExpressionMutationchanged cysteine 102 to alaninePromoterUbiquitinAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLGCV
Plasmid#49862PurposeExpression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).DepositorInsertfirefly luciferase luc+ fused to GFP (S65C)
UseTagsGFP (S65C)ExpressionWormMutationPromotersur-5Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUTKan-3xHA-GFP-TOM5
Plasmid#130674PurposeUbiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of plantaDepositorInsertTranslocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
UseTags3xHA and sGFPExpressionPlantMutationPromoterUbiquitin10Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEN-dCas9-CDA1-UGI
Plasmid#196248PurposePlasmid containing promoter of RecA from Xanthomonas oryzae driving expression of dead Cas9, cytosine deaminase CDA1, and glycosylase inhibitorDepositorInsertattL4-RecApro-dCas9-CDA1-UGI-attR1
UseTagsExpressionBacterialMutationPromoterRecA promoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
LZF1735 pAAV_hSyn-DiO-somArchon1-EGFP-P2A-somCheRiff
Plasmid#126512PurposeOptopatch4. Simultaneous optical recording and manipulation of electrical activity in neuronsDepositorInsertsomArchon1-EGFP-P2A-somCheRiff
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29a-hNABP2
Plasmid#128307PurposeExpress hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tagDepositorInsertHuman Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
UseTagsHisExpressionBacterialMutationY85C- please see depositor comments belowPromoterT7Available sinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)mGAT1-0-GFP
Plasmid#41662DepositorInsertMus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)
UseTagsGFPExpressionMammalianMutationC-terminal hydrophobic isoleucine residue added a…PromoterCMVAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFK210B-AtMIR390a-B/c
Plasmid#51777PurposePlant expression vector (35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursorDepositorInsertAtMIR390a-B/c
UsePlant expressionTagsExpressionMutationA. thaliana MIR390a precursor sequence including …PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-B/c
Plasmid#51773PurposePlant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursorDepositorInsertAtTAS1c-B/c
UsePlant expressionTagsExpressionMutationA. thaliana TAS1c precursor sequence including a …PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-surrogate-reporter
Plasmid#65624PurposeFluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.DepositorInsertattP
UseBxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
hP2X3-cALFA
Plasmid#160499PurposeFor the expression of human P2X3 with ALFA tag at C-terminus in mammalian cellsDepositorInserthuman P2X3 (P2RX3 Synthetic, Human)
UseTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationΔN5ΔC33-T13P/S15V/V16IPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmApple-GNG2
Plasmid#133857PurposeMammalian expression of GNG2 N-terminally tagged with mAppleDepositorInsertGNG2 (GNG2 Human)
UseTagsmAppleExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only