We narrowed to 5,882 results for: pcDNA 3.1

1. NSP3C-FR-2Strep

   Plasmid

   #210312

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SARS-CoV-2 N-2Strep

   Plasmid

   #210376

   Purpose

   Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-COV-2 N (N SARS-CoV-2)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TLNRD1_2T_eGFP

   Plasmid

   #208883

   Purpose

   Express eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   TLNRD1 (TLNRD1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutations L191T and A225T

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TLNRD1_2E_eGFP

   Plasmid

   #208884

   Purpose

   Express eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   TLNRD1 (TLNRD1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   mutations K192E and R233E

   Promoter

   Available since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. REEP6-2Strep

   Plasmid

   #210351

   Purpose

   Expresses REEP6 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   REEP6 (REEP6 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFH2.17_GagZip-MCP-dpol-P2A-eGFP

   Plasmid

   #205532

   Purpose

   Express HIV GagZip-MCP-dpol-P2A-eGFP

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Gag-MCP-dpol
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Nucleocapsid replaced with leucine zipper, pol fr…

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eGFP-RAPH1-F4

   Plasmid

   #194863

   Purpose

   Express the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acids 1062-1250

   Promoter

   CMV

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eGFP-RAPH1-F3

   Plasmid

   #194862

   Purpose

   Express the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acids 868-1062

   Promoter

   CMV

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. eGFP-RAPH1-F2

   Plasmid

   #194861

   Purpose

   Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   amino acids 535-868

   Promoter

   CMV

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pClathrin-miRFP718nano

    Plasmid

    #195747

    Purpose

    Clathrin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-clathrin light chain
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTubulin-miRFP718nano

    Plasmid

    #195746

    Purpose

    Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human alpha-tubulin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

    Plasmid

    #194725

    Purpose

    based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

    Depositor

    Rudolf Jaenisch

    Article

    Haiting et al bioRxiv

    Insert

    AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GFP-CBS (H65R)

    Plasmid

    #182924

    Purpose

    Mammalian expression plasmid for human CBS mutant H65R

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed histidine 65 to arginine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-mVenusC-14-HP1b Chromo W42A

    Plasmid

    #191399

    Purpose

    W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    FLAG-mVenusC-14-HP1b Chromo W42A
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    W42A

    Promoter

    CMV

    Available since

    Dec. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVH270-Tier1-OTtgO2-PCMVmin2-SEAP-p2A-iRFP670

    Plasmid

    #169592

    Purpose

    Tier-1 vector encoding PTtgO2-driven SEAP-p2A-iRFP670 expression (OTtgO2-PCMVmin-2-SEAP-p2A-iRFP670-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    phloretin-controlled SEAP and iRFP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TtgO2-PCMVmin-2

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVH266-Tier1-OTetO7-PCMVmin2-SEAP-sTRSV

    Plasmid

    #169589

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR sTRSV ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRsTRSV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTS2393-Tier1-PhCMV-p2a_fastFT

    Plasmid

    #169530

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, fast maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNIR-Fb21

    Plasmid

    #184679

    Purpose

    NIR-Fb to spike-Cov-19 RBD

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    NIR-Fb21 (S Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVH63-Tier1-PMLP-Citrine_p2a_SEAP

    Plasmid

    #169557

    Purpose

    Tier-1 vector encoding PMLP-driven Citrine-p2A-SEAP expression (PMLP-Citrine-p2A-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PMLP-driven citrine-P2A-SEAP fusion construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PMLP

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVH267-Tier1-OTetO7-PCMVmin2-SEAP-env140

    Plasmid

    #169590

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a 3'UTR env140 ribozyme (OTetO7-PCMVmin-2-SEAP-3'UTRenv140-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVH65-Tier1-PTK-Citrine_p2a_SEAP

    Plasmid

    #169559

    Purpose

    Tier-1 vector encoding PTK-driven Citrine-p2A-SEAP expression (PTK-Citrine-p2A-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PTK-driven citrine-P2A-SEAP fusion construct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PTK

    Available since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTS2392-Tier1-PhCMV-p2a_medFT

    Plasmid

    #169529

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT fused to a "self-cleaving" peptide p2a (PhCMV-p2a_medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused fluorescent timer, medium maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTS2388-Tier1-PhCMV-p2a_Ypet

    Plasmid

    #169525

    Purpose

    Tier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet fused to a "self-cleaving" peptide p2a (PhCMV-p2a_YPet-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused yellow fluorescent protein Ypet
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTS2387-Tier1-PhCMV-p2a_mTagBFP2

    Plasmid

    #169524

    Purpose

    Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTS2389-Tier1-PhCMV-p2a_mRuby

    Plasmid

    #169526

    Purpose

    Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mRuby2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven P2A-fused enhanced yellow fluorescent protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVH321-2-Tier1-PhCMV-NLS-dCas9-3xNLS

    Plasmid

    #169596

    Purpose

    Tier-1 vector encoding PhCMV-driven NLS-dCas9-3xNLS expression (PhCMV-NLS-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pTS2406-Tier1-PhCMV-NTEV

    Plasmid

    #169501

    Purpose

    Tier-1 vector encoding PhCMV-driven N-terminal fragment of a split tabacco etch virus protease splitTEV (PhCMV-N-TEV-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven N-terminal fragment of split tobacco etch virus protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTS1024-Tier1-PRPBSA-YPet-p2A-PuroR

    Plasmid

    #169499

    Purpose

    Tier-1 vector encoding PRPBSA-driven YPet in combination with a marker for puromycine selection (PRPBSA-YPet-p2A-PuroR-pA)

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PRPBSA-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PRBSA

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTS1047-Tier1-PhCMV-YPet-p2A-PuroR

    Plasmid

    #169498

    Purpose

    Tier-1 vector encoding PhCMV-driven YPet in combination with a marker for puromycin selection (PhCMV-YPet-p2A-PuroR-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven YPet-P2aA-PuroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pOME0135

    Plasmid

    #178397

    Purpose

    KSHV Kaposincluster-RevCompl-Syn

    Depositor

    Dirk Dittmer

    Article

    Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

    Insert

    Kaposincluster-RevCompl-SYN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge