We narrowed to 27,269 results for: Tat

1. His SUMO TEV WT SPOP 28-359

   Plasmid

   #194115

   Purpose

   Wild type His-SUMO SPOP expression plasmid

   Depositor

   Tanja Mittag

   Article

   Thomasen et al Elife. 2023 Mar 1;12:e84147. doi: 10.755

   Insert

   SPOP (SPOP Human)
   

   Use

   Tags

   6His-SUMO-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV.CMV.flex.hM3Dq.Cherry

   Plasmid

   #170360

   Purpose

   Cre-inducible lentiviral vector expressing hM3Dq

   Depositor

   Johan Jakobsson

   Article

   Klawonn et al Immunity. 2021 Feb 9;54(2):225-234.e6. d

   Insert

   flex.hM3Dq.Cherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Jan. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINDUCER13 miR-200c-141

   Plasmid

   #81020

   Purpose

   Lentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.

   Depositor

   Thomas Westbrook, Jeffrey Rosen

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   miR-200c (MIR200C Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-6xHis-GFP(+36)

   Plasmid

   #199165

   Purpose

   used to express a supercharged GFP variant in E. coli

   Depositor

   Allie Obermeyer

   Article

   Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

   Insert

   GFP(+36)
   

   Use

   Tags

   MGHHHHHHGGA

   Expression

   Mutation

   E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

   Promoter

   T7

   Available since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEK39

   Plasmid

   #210599

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   SpH2Bpro-Hshph-G4S-HsmRuby3-ScADH1ter
   

   Use

   Tags

   mRuby3

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SpH2Bpro

   Available since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFLAG-Halo-Dyskerin

   Plasmid

   #207578

   Purpose

   CRISPR-Cas9 genome editing encoding a FLAG-HaloTag at the endogenous dyskerin locus

   Depositor

   Jens Schmidt

   Article

   Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

   Insert

   HaloTag DKC1
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AKARdual

   Plasmid

   #99869

   Purpose

   Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   AKAR4
   

   Use

   Tags

   LSSmOrange and mKate2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-UbC-FKRP-EF1a-BSD

   Plasmid

   #205150

   Purpose

   Express UbC-driven FKRP and EF-1a driven BSD

   Depositor

   Monkol Lek

   Insert

   Fukutin-related protein (FKRP Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UbC

   Available since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-AcrIIA4-NLS(SV40) (KAC200)

   Plasmid

   #133801

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA4 with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized AcrIIA4
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mAIRN HO

    Plasmid

    #207411

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mAIRN CD

    Plasmid

    #207412

    Purpose

    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).

    Depositor

    Karlene Cimprich

    Article

    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do

    Insert

    mAIRN
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEM112B

    Plasmid

    #210602

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2A/Bpro (divergent)

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-mApple-6xHp

    Plasmid

    #217376

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    mApple-6x Hp NC
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mutation

    Promoter

    CMV

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Calnexin-HT-mGold

    Plasmid

    #183987

    Purpose

    Expresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.

    Depositor

    Pablo Rivera-Fuentes

    Article

    Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

    Insert

    Calnexin (CANX Human)
    

    Use

    Tags

    HaloTag, mGold

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-Hyg-RFPKDEL

    Plasmid

    #138660

    Purpose

    Reporter plasmid expressing the ER-located RFP

    Depositor

    Bertrand Collet

    Article

    Dehler et al J Immunol. 2019 Jul 15;203(2):465-475. d

    Insert

    RFP-KDEL
    

    Use

    Tags

    KDEL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-AcrIIA5-NLS(SV40) (KAC203)

    Plasmid

    #133802

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized AcrIIA5 with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized AcrIIA5
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and T7

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hy_pMT Laccase2 MCS Exon Vector

    Plasmid

    #69884

    Purpose

    Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    Laccase2 (stw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)

    Plasmid

    #73404

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1α-FRT-FLEX-GtACR1-EYFP

    Plasmid

    #114370

    Purpose

    expresses Flpo recombinase-dependent GtACR1

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR1-EYFP (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW-CCNE1-HA-puro

    Plasmid

    #212669

    Purpose

    Express tagged cyclin E1

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGH020_sgRNA_G418-GFP

    Plasmid

    #85405

    Purpose

    hu6 driven sgRNA vector with G418 and GFP selectable markers

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    G418 resistance and GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. a1Ic-HE3-pcDNA3

    Plasmid

    #45810

    Depositor

    Edward Perez-Reyes

    Article

    Gomora et al Biophys J. 2002 Jul;83(1):229-41.

    Insert

    CACNA1I (CACNA1I Human)
    

    Use

    Making crna for oocyte injections

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-HA-GASC1

    Plasmid

    #24214

    Purpose

    Mammalian expression of human JMJD2C with HA tag

    Depositor

    Kristian Helin

    Article

    Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

    Insert

    GASC1 (KDM4C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TRE-UBC-H2-Kb SCT-T2A-GFP

    Plasmid

    #205454

    Purpose

    lentiviral plasmid for expression of mouse H2-Kb SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    H2-Kb SCT (H2-K1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR221-hSOX10

    Plasmid

    #24749

    Depositor

    William Pavan

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    (sex determining region Y)-box 10 (SOX10 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    wildtype sequence

    Promoter

    Available since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

26. GB4542

    Plasmid

    #215261

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GB4401

    Plasmid

    #215252

    Purpose

    Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Sec61b-HA-mNG21-10

    Plasmid

    #141213

    Purpose

    Membrane Tethered Split Fluorescent Reporter for ER Retragrade

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    Sec61b-HA-mNG21-10
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-STAG2-wildtype

    Plasmid

    #31972

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CMV

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-MBII85-5'SS mut

    Plasmid

    #67631

    Purpose

    MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons. To improve snoRNA expression efficiency 5' splice site was mutated to consensus sequence.

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

    Insert

    MBII85 snoRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutation in 5'ss

    Promoter

    Available since

    Aug. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MBP-TEVcs(ENLYFQ/G)-His6-uTEV1Δ(220-242)-R5

    Plasmid

    #135455

    Purpose

    Bacterial expression of uTEV1Δ protease

    Depositor

    Alice Ting

    Article

    Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

    Insert

    MBP-TEVcs(ENLYFQ/G)-His6-TEV1Δ(220-242)-R5
    

    Use

    Tags

    MBP, His6

    Expression

    Bacterial

    Mutation

    S219V mutation improves stability, S153N mutation…

    Promoter

    tac promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Hy_pMT Flag-URH49 WT

    Plasmid

    #110127

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available since

    April 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLentiX2-PURO-shLKB1-Ms

    Plasmid

    #61231

    Purpose

    Lentivirus expressing shRNA to mouse LKB1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    shLkb1 (Ms/Hu) (Stk11 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    H1/TO

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pCMV-Tag4A-MITF-M (wt)

    Plasmid

    #31151

    Depositor

    Yardena Samuels

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    MITF-M (MITF Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FAB(ER-LD)

    Plasmid

    #214424

    Purpose

    Detection of ER-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Use

    Tags

    CFAST-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-mCAMK-RFP

    Plasmid

    #22908

    Depositor

    Edward Callaway

    Article

    Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

    Insert

    mouse alpha-calcium/calmodulin-dependent protein kinase II promoter (Camk2a Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTK23

    Plasmid

    #37405

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

    Insert

    Neuromodulin (GAP43 Human)
    

    Use

    Tags

    S-tag, TEV, and mCherry

    Expression

    Mammalian

    Mutation

    Contains the first 20aa of GAP43 (neuromodulin)

    Promoter

    Available since

    July 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDS2142

    Plasmid

    #184273

    Purpose

    Tet-off genome insertion system

    Depositor

    Dominique Sanglard

    Article

    Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

    Insert

    CatTA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-AcGFP-progerin

    Plasmid

    #86947

    Purpose

    Construct used to synthesize progerin mRNA. Please note the plasmid contains LMNA gene with nucleotide C1824T mutation.

    Depositor

    Alex Hewitt

    Insert

    AcGFP-progerin (LMNA Human)
    

    Use

    AAV

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    progerin sequence is LMNA gene with nucleotide C1…

    Promoter

    CMV and T7

    Available since

    June 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLentiX2-PURO-shLKB1-Hu

    Plasmid

    #61242

    Purpose

    Lentivirus expressing shRNA to human LKB1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    LKB1 (STK11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge