We narrowed to 4,380 results for: 1-Oct;

1. pEF1a-hMLH1dn (og codon)

   Plasmid

   #174823

   Purpose

   Mammalian expression of human MLH1dn (original codon usage)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1dn (original codon usage) (MLH1 Human)
   

   Expression

   Mammalian

   Mutation

   deletion of residues 754-756

   Promoter

   EF1a

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-SARS-CoV-2-Nsp3PLpro

   Plasmid

   #204208

   Purpose

   Mammalian expression of SARS-CoV-2 Nsp3

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
   

   Tags

   FLAGx1

   Expression

   Mammalian

   Mutation

   PLpro of Nsp3

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ACE2-Fc

   Plasmid

   #164222

   Purpose

   Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   ACE2 extracellular domain (ACE2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-REX-GECO1

   Plasmid

   #61246

   Purpose

   Expresses REX-GECO1 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

   Insert

   REX-GECO1
   

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1: P60R, V61W, R6…

   Promoter

   CMV

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-VCPIP1

   Plasmid

   #85671

   Purpose

   Expression of VCPIP1 with N-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   VCPIP1 (VCPIP1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Foxf1 HA pShuttle

   Plasmid

   #122120

   Purpose

   Expression of Foxf1 in mammalian cells

   Depositor

   Paul Herring

   Article

   Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

   Insert

   Foxf1 (Foxf1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. U6-CjCas9-MS2gRNA-Backbone

   Plasmid

   #210711

   Purpose

   This plasmid express MS2 loop containing Cj Cas9 gRNA

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MS2 loop containing Cj Cas9 gRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-MTS-WT-c-Src-FLAG

   Plasmid

   #44652

   Depositor

   Yoshimi Homma

   Article

   Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

   Insert

   v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
   

   Tags

   FLAG tag and mitochondria-targeting sequence (res…

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. TP63-T2A-Rox-EF1a-Rox-Venus-NLS repair template

   Plasmid

   #167971

   Purpose

   Tp63 reporter targeting repair template

   Depositor

   Emma Rawlins

   Article

   Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

   Insert

   Tp63 (TP63 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCK301

    Plasmid

    #87767

    Purpose

    E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest to express using L-rhamnose, three terminators, ampR, pBR322 origin, lacI

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Insert

    PrhaBAD-sfGFP
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-MTS-CA-c-Src-FLAG

    Plasmid

    #44654

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed Tyr 530 to Phe

    Promoter

    CMV promoter

    Available Since

    May 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV-hSyn-REX-GECO1

    Plasmid

    #61248

    Purpose

    Expresses REX-GECO1 in neurons

    Depositor

    Robert Campbell

    Article

    Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

    Insert

    REX-GECO1
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Substitutions relative to R-GECO1: P60R, V61W, R6…

    Promoter

    hSyn

    Available Since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. ER-CanlonicSF

    Plasmid

    #178343

    Purpose

    To explore endoplasmic reticulum lactate dynamics

    Depositor

    Alejandro San Martín

    Article

    Aburto et al ACS Sens. 2022 Oct 28. doi: 10.1021/acss

    Insert

    ER-CanlonicSF
    

    Tags

    ER Retention Signal, ER Signal Exon 1, and ER Sig…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-MTS-KD-c-Src-FLAG

    Plasmid

    #44653

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed lysine 298 to methionine

    Promoter

    CMV promoter

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. SFTPC-T2A-Rox-EF1a-Rox-Venus-NLS repair template

    Plasmid

    #167943

    Purpose

    SFTPC reporter targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    SFTPC (SFTPC Human)
    

    Use

    CRISPR; Donor template

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    EF1a

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-FKBP-C-mSOG-J-alpha-IRES-mCherry

    Plasmid

    #136603

    Purpose

    Encodes FKBP tagged with the C-terminal fragment of split-miniSOG (mSOG-Ja95-140).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    FKBP-C-mSOG-J-alpha
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-EF1a-mTagRFP-T-CAAX

    Plasmid

    #167973

    Purpose

    AAVS1 targeting repair template

    Depositor

    Emma Rawlins

    Article

    Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

    Insert

    AAVS1 (PPP1R12C, AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFP-T-CAAX

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SARS-CoV-2 Nsp3TMC111A

    Plasmid

    #204207

    Purpose

    Mammalian expression of SARS-CoV-2 Nsp3

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
    

    Tags

    FLAGx1

    Expression

    Mammalian

    Mutation

    Cys to Ala in active site of PLpro

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-SARS-CoV-2 Nsp3TM

    Plasmid

    #204206

    Purpose

    Mammalian expression of SARS-CoV-2 Nsp3

    Depositor

    Allan Weissman

    Article

    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

    Insert

    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/) (ORF1ab )
    

    Tags

    FLAGx1

    Expression

    Mammalian

    Mutation

    none, from obtained clone

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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