We narrowed to 12,321 results for: BASE

1. pLVX-EF1a-PTDSS1-IRES-Puromycin

   Plasmid

   #134667

   Purpose

   Expresses EGFP-tagged Mitochondria-associated ER membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   PTDSS1 (PTDSS1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. VV229: GCaMP6f in fck

   Plasmid

   #58514

   Purpose

   Expresses GCaMP6f under the a-CamKII promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   GCaMP6f
   

   Use

   Lentiviral

   Tags

   C-terminal FCYENEV (ER export motif); this was no…

   Expression

   Mammalian

   Mutation

   Promoter

   a-CamKII

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-APOBEC1-YTHmut

   Plasmid

   #200159

   Purpose

   For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification

   Depositor

   Kate Meyer

   Article

   Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882

   Insert

   APOBEC1-YTHmut
   

   Use

   Tags

   6xHis, HA, and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E coli expression

   Promoter

   LacZ

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-N-Blast-miRFP670nano3

   Plasmid

   #207680

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pFUS_B3

   Plasmid

   #31020

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUS_B1

   Plasmid

   #31018

   Depositor

   Adam Bogdanove, Daniel Voytas

   Article

   Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

   Insert

   LacZ + BsaI restriction sites
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTomatoSlicer

   Plasmid

   #221085

   Purpose

   Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. p70a-T7RNAP

   Plasmid

   #105683

   Purpose

   Expresses T7 polymerase in cell-free TXTL.

   Depositor

   Chase Beisel

   Article

   Maxwell et al Methods. 2018 Jul 1;143:48-57. doi: 10.1

   Insert

   T7 polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGG-TurboID

   Plasmid

   #209391

   Purpose

   Transiently expressing TurboID in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID
   

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK485 (3mAID-Hygro)

    Plasmid

    #214397

    Purpose

    3mAID for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    3mAID-Hygro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. mEGFP-2xbiRhoBAST

    Plasmid

    #196349

    Purpose

    Expression of mEGFP mRNA with 2xbiRhoBAST in 3'UTR

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    mEGFP-2xbiRhoBAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCarmine in pRSETB

    Plasmid

    #109484

    Purpose

    bacterial expression vector for mCarmine, a far red emitting fluorescent protein (excitation: 603; emission: 675)

    Depositor

    Oliver Griesbeck

    Article

    Fabritius et al Cell Chem Biol. 2018 Dec 20;25(12):1554-

    Insert

    mCarmine
    

    Use

    Tags

    N-terminal histidine tag in vector

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRDA_449

    Plasmid

    #179093

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBJ1

    Plasmid

    #167130

    Purpose

    A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

    Depositor

    Akira Mine

    Article

    Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

    Insert

    luxCDABE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Frr promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPpT4- PGK1prom-TIR1-FLAG

    Plasmid

    #189724

    Purpose

    Enables expression of OsTIR1 from the PGK1 promoter, used to implement the AID system in Komagataella phaffii (original name pAEA454)

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1 promoter

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK448 (BromoTag-BSD)

    Plasmid

    #214378

    Purpose

    BromoTag for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BromoTag-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK455 (dTAG-mClover-BSD)

    Plasmid

    #214385

    Purpose

    dTAG-mClover for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    dTAG-mClover-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK456 (dTAG-mCherry-BSD)

    Plasmid

    #214386

    Purpose

    dTAG-mCherry for C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    dTAG-mCherry-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. LLhG-pHG165a E62K

    Plasmid

    #90051

    Purpose

    Constitutive expression of a chimeric LacI:GalR transcription repressor

    Depositor

    Liskin Swint-Kruse

    Article

    Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

    Insert

    LLhG/E62K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E62K

    Promoter

    Iq

    Available since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pdCas9+sgRNA expression vector precursor

    Plasmid

    #171798

    Purpose

    PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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