We narrowed to 12,112 results for: LEA

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pBABEpuro-Flag-YAP2

Plasmid

#27472

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pHJ425

Plasmid

#20683

Depositor

Stephen Shaw

Publication

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn; and change…

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
PSuperPuroRetro-1660s(hPot1)

Plasmid

#11136

Depositor

Christopher Counter

Publication

Veldman et al Curr Biol. 2004 Dec 29. 14(24):2264-70.

Insert

RNAi against Human Protection of Telomeres 1 (control) (POT1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pαH-S-GSAS-OMICRON

Plasmid

#180423

Purpose

Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS

Depositor

Priyamvada Acharya

Publication

Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

Insert

Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron

Plasmid

#235087

Purpose

GFP splicing reporter with FRG1 intron

Depositor

Mitchell Guttman

Publication

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter FRG1-intron (FRG1 Human)

Use

AAV

Expression

Mammalian

Promoter

custom bidirectional

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron

Plasmid

#235086

Purpose

GFP splicing reporter with CORO1B intron

Depositor

Mitchell Guttman

Publication

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

BFP-GFP-bidirectional MS2 splicing reporter CORO1B intron (CORO1B Human)

Use

AAV

Expression

Mammalian

Promoter

custom bidirectional

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pK27Sumo_His-SUMO-nsp7 (SARS-CoV-2)

Plasmid

#169186

Purpose

To express His-SUMO-nsp7 (SARS-CoV-2) in E. coli

Depositor

John Diffley

Publication

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

14His-SUMO-nsp7 (ORF1ab SARS-CoV-2)

Tags

14His-SUMO (Ulp1-cleavable)

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T5

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET-28a-His6-SUMO-Munc18-1wt

Plasmid

#135550

Purpose

Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Publication

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18-1wt

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGL4.10 Dub3

Plasmid

#72684

Purpose

luciferase reporter with Dub3 promoter

Depositor

Domenico Maiorano

Publication

van der Laan et al PLoS One. 2014 Apr 2;9(4):e93663. doi: 1

Insert

Dub3 promoter (Usp17le Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

Dub3

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMV14_NHP2L1(15.5kD)

Plasmid

#73065

Purpose

expression clone for human NHP2L1 (15.5kD) with C-terminal 3xFLAG-tag

Depositor

Stefan Stamm

Publication

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

NHP2L1 (15.5kD) (SNU13 Human)

Tags

3xFLAG

Expression

Mammalian

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

Plasmid

#107319

Purpose

Expresses SpCas9 fused to dSaCas9 in mammalian cells

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9-SaCas9 fusion

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…

Promoter

CMV IE94

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-cDNA5-FRT/TO-3*Flag-APEX2 C-term

Plasmid

#182926

Purpose

Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

Depositor

Benjamin Blencowe

Publication

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Type

Empty backbone

Tags

APEX2 and FLAG

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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BB3cN_pGAP_23*_pLAT1_Cas9

Plasmid

#104906

Purpose

hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

Depositor

Brigitte Gasser

Publication

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Inserts

HH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)

Use

CRISPR

Expression

Yeast

Available since

Feb. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

Plasmid

#87827

Purpose

Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.

Depositor

Andrea Musacchio

Publication

Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1

Inserts

NLS
PTS1

Tags

EGFP and mCherry

Expression

Mammalian

Promoter

CMV promoter, tetracycline operator

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA

Plasmid

#176538

Purpose

Mammalian expression of nuclear localized AwaIscB

Depositor

Feng Zhang

Publication

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Tags

Nucleoplasmin NLS-3xHA and SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75240

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Ikaros-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75241

Purpose

CRISPR/Cas9 plasmid against human Ikaros

Depositor

Ria Baumgrass

Publication

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against human Ikaros (IKZF1 Human)

Use

CRISPR

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-all

Plasmid

#21653

Depositor

David Bartel

Publication

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated let-7 and miR103/107 microRNA binding sit…

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCin4-3xFLAG-MKL1 S454A

Plasmid

#19844

Depositor

Ron Prywes

Publication

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S454A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 454 to Alanine

Available since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pLC13

Plasmid

#78494

Purpose

Expression of Mu-gam under the pBAD promoter

Depositor

David Bikard

Publication

Cui et al Nucleic Acids Res. 2016 Apr 8. pii: gkw2

Insert

Mu-gam (Mup10 )

Expression

Bacterial

Available since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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