10,178 results

1. pTn7-SCOUT22

   Plasmid

   #200996

   Purpose

   pLv2-pUC18R6KT-miniTn7-Km, KmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTn7-SCOUT23

   Plasmid

   #200997

   Purpose

   pLv2-pUC18R6KT-miniTn7-Tc, TcR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTn7-SCOUT24

   Plasmid

   #200998

   Purpose

   pLv2-pUC18R6KT-miniTn7-aad9, SpR, StR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTn7-SCOUT20

   Plasmid

   #200990

   Purpose

   pL0V-Lv2-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EgI-NeoR

   Plasmid

   #207175

   Purpose

   Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoR

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTH-TelL15-mVenus

   Plasmid

   #49638

   Purpose

   TALEColor-mVenus-Telomere

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDRf1-4CL5-DsRed (JBEI-15461)

   Plasmid

   #87935

   Purpose

   Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

   Depositor

   Dominique Loque

   Article

   Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB443

   Plasmid

   #74829

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBEN1-SGC

   Plasmid

   #39127

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Nicola Burgess-Brown

   Type

   Empty backbone

   Use

   Tags

   Strep tag and SET1

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pCUP1pNuiHA kanMX CEN

    Plasmid

    #131168

    Purpose

    for ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiae

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    Nui-HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDEST-Spe4-Kozak-Snaptag-RfA

    Plasmid

    #186385

    Purpose

    Destination vector for the expression of a fluorescent fusion of N-ter Snap tag and a protein of interest into embryos, by mRNA microinjection

    Depositor

    Patrick Lemaire

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+/N-SNAPf

    Plasmid

    #184415

    Purpose

    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2+/C-SNAPf

    Plasmid

    #184416

    Purpose

    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Expression

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Promoter

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSUM-Kan-MCS2

    Plasmid

    #109289

    Purpose

    Expresses high levels of protein in mycobacteria

    Depositor

    Nicolai van Oers

    Article

    Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    pSUM-kan-MCS1-gfp was modified to reorient the Ba…

    Promoter

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEN_hUmiRc2

    Plasmid

    #25747

    Purpose

    Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCellFree_G07

    Plasmid

    #67141

    Purpose

    Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His-MYC and mCherry

    Expression

    Mutation

    Promoter

    Available Since

    May 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRA-GW

    Plasmid

    #209333

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET mRuby2 LIC cloning vector (u-mRuby2)

    Plasmid

    #86932

    Purpose

    pET LIC cloning vector for yORF-mRuby2

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    mRuby2

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMINTC3GH (Kan)

    Plasmid

    #110076

    Purpose

    Analogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pACEC3GH (Apr)

    Plasmid

    #110085

    Purpose

    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pXNgate21-3HA

    Plasmid

    #105078

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. JL202: pMVP/mCherry-DEST

    Plasmid

    #121853

    Purpose

    pMVP destination vector, empty expression vector backbone w/ mCherry selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAG423GPD-ccdB-HA

    Plasmid

    #14246

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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24. JL002: pMVP/eGFP-DEST

    Plasmid

    #121852

    Purpose

    pMVP destination vector, empty expression vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMpGWB136

    Plasmid

    #68590

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pC23-LIC-His-FLAG

    Plasmid

    #165477

    Purpose

    pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His

    Depositor

    Renato Bruni

    Article

    Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

    Type

    Empty backbone

    Use

    Tags

    TEV-FLAG-10x His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAM-AAV-mSncg-0.27kb-EGFP

    Plasmid

    #153185

    Purpose

    Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

    Depositor

    Yang Hu

    Article

    Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYPQ143-ZmUbi

    Plasmid

    #138107

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid

    #105801

    Purpose

    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    ORF1: FLAG-3C; ORF2: mClover3-TEV

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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30. pQCXIN X2 DEST (w310-1)

    Plasmid

    #17399

    Purpose

    Retroviral Gateway destination vector, RNAi, 3’LTR insertion, Neo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    RNAi and Retroviral; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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