We narrowed to 7,519 results for: Lif

1. pCDNA5/FRT/TO_CEP41_HaloTag9

   Plasmid

   #169331

   Purpose

   Expression of HaloTag9 located at the microtubules of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   CEP41-HaloTag9 (CEP41 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET24a-LaM4-1xTS

   Plasmid

   #162778

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. ins:BB1.0L; cryaa:RFP

   Plasmid

   #110283

   Purpose

   A zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Insert

   Brainbow1.0L
   

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   insulin

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. C25-pLAC

   Plasmid

   #45283

   Purpose

   lacritin-intein fusion plasmid coding for truncated lacritin without signal peptide

   Depositor

   Gordon Laurie

   Article

   Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

   Insert

   pLAC-C25 (LACRT Human)
   

   Use

   Protein expression vector

   Tags

   Intein

   Expression

   Bacterial

   Mutation

   contains aa20-113 of lacritin

   Promoter

   T7

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMH-HT_SsCHAD-H29A-R32A-R36A-R69A

   Plasmid

   #127787

   Purpose

   Expresses a SsCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Use

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   mutations in His29, Arg32, Arg36 and Arg69 to Ala

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV Tmem53-V5

   Plasmid

   #175142

   Purpose

   Lentiviral expression of mouse Tmem53-V5

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Tmem53 (Tmem53 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDule-Acd82

   Plasmid

   #174099

   Purpose

   Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

   Depositor

   Ryan Mehl

   Article

   Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

   Insert

   Acridone 82 tRNA synthetase and tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N311S, C313G, V366A, W382T

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. ins:MCS2; cryaa:RFP

   Plasmid

   #110286

   Purpose

   A zebrafish insulin promoter containing vector with a second multiple cloning sites. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Type

   Empty backbone

   Use

   Zebrafish

   Tags

   Expression

   Mutation

   Promoter

   insulin

   Available since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPC-CHES1L

   Plasmid

   #53743

   Purpose

   long isoform of human CHES1

   Depositor

   Gerardo Ferbeyre

   Article

   Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

   Insert

   CHES1 (FOXN3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pGEMT-Tyrosinase-bait-T2a-Cherry-pA

    Plasmid

    #111155

    Purpose

    knock in bait for Axolotl Tyrosinase locus

    Depositor

    Elly Tanaka

    Article

    Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

    Insert

    Tyrosinase-bait-T2a-Cherry-pA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZE21-APX(W41F) dimeric

    Plasmid

    #58199

    Purpose

    Bacterial expression of APX W41F under control of TetR

    Depositor

    Alice Ting

    Article

    Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

    Insert

    pea APX W41F
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    W41F

    Promoter

    pL(tetO-1)

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV T274V S392A KSR1 IRES GFP

    Plasmid

    #25979

    Depositor

    Rob Lewis

    Article

    Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed threonine 274 to valine and serine 392 to…

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pBMN iRFP670-NDP52(C443K)

    Plasmid

    #119695

    Purpose

    For mammalian expression of iRFP-tagged UBD mutant NDP52

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    C443K

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEXP5-NT-CuSeCat EE

    Plasmid

    #42569

    Depositor

    Ivan Korendovych

    Article

    Mack et al J Biol Inorg Chem. 2013 Apr;18(4):411-8.

    Insert

    mutated calmodulin gene
    

    Use

    Tags

    Polyhistidine (6×His) region, TEV recognition sit…

    Expression

    Bacterial

    Mutation

    changed Phenylalanine 92 to Glutamine acid, Valin…

    Promoter

    T7

    Available since

    March 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. p252 pPr-CREM/CMV-STOP-luc

    Plasmid

    #8406

    Purpose

    Expression of modified Cre from androgen-inducible probasin promoter and LOX-dependent conditional reporter cassette.

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM/lox/luciferase
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    Probasin promoter, CMV

    Available since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

16. mlp-hCHES1sh

    Plasmid

    #51845

    Purpose

    shRNA against CHES1

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    shRNA against CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pWZL-rCHES1

    Plasmid

    #51847

    Purpose

    encodes a CHES1 mRNA that is resistant to shRNA against CHES1

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pTRC-APX(W41F) dimeric

    Plasmid

    #58499

    Purpose

    Bacterial expression of APX W41F inducible by IPTG

    Depositor

    Alice Ting

    Article

    Dwyer et al Proc Natl Acad Sci U S A. 2014 May 20;11

    Insert

    pea APX W41F (dimeric)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    W41F

    Promoter

    trc

    Available since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-IRES-PURO-RAC1-R66A

    Plasmid

    #241369

    Purpose

    Lentiviral expression of mutant Rac1

    Depositor

    Gaudenz Danuser

    Article

    Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R66A

    Promoter

    CMV

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-IRES-PURO-RAC1-Y64F

    Plasmid

    #241370

    Purpose

    Lentiviral expression of mutant Rac1

    Depositor

    Gaudenz Danuser

    Article

    Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Y64F

    Promoter

    CMV

    Available since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. XLone-CRNDEUCE (5 kb)

    Plasmid

    #242179

    Purpose

    Express doxycycline inducible 5 kb UCE-containing CRNDE in mammalian cells

    Depositor

    Joshua Mendell

    Article

    Lee et al Mol Cell. 2025 Apr 17;85(8):1543-1560.e1

    Insert

    CRNDEUCE (CRNDE Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCAGGS_hTRPV1-p2A-tdTomato

    Plasmid

    #238923

    Purpose

    Expression of the human TRPV1 channel with tdTomato as a reporter in mammalian cells

    Depositor

    Vsevolod Belousov

    Article

    Maltsev et al Cell Mol Life Sci. 2024 Oct 25;81(1):437

    Insert

    hTRPV1-p2A-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGADT7_Sr62TK-Kinase1

    Plasmid

    #233529

    Purpose

    Expresses Sr62TK-Kinase1 in yeast

    Depositor

    Brande Wulff

    Article

    Chen et al bioRxiv 2024.08.30.610287

    Insert

    Sr62TK-Kinase1
    

    Use

    Tags

    GAL4 AD, HA tag

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Aug. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTRIP-SFFV-tagBFP-2A-IE1

    Plasmid

    #235541

    Purpose

    To express tagBFP-2A-IE1

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    IE1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTRIP-SFFV-tagBFP-2A-FLAG-coORF63

    Plasmid

    #235544

    Purpose

    To express tagBFP-2A-FLAG-coORF8 (co = codon-optimized)

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    ORF63
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLentiCRISPRv2 Neo-MORC3_gRNA_1

    Plasmid

    #235529

    Purpose

    gRNA against human MORC3

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    MORC3 (MORC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLentiCRISPRv2 Neo-MORC3_gRNA_3

    Plasmid

    #235530

    Purpose

    gRNA against human MORC3

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    MORC3 (MORC3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTRIP-SFFV-tagBFP-2A-coICP0

    Plasmid

    #235536

    Purpose

    To express tagBFP-2A-coICP0 (co = codon-optimized)

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    ICP0
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTRIP-SFFV-tagRFP657-2A-coICP0

    Plasmid

    #235538

    Purpose

    To express tagRFP657-2A-coICP0 (co = codon-optimized)

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

    Insert

    ICP0
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-flag-IARS2-Δ1-48

    Plasmid

    #232341

    Purpose

    Expresses human IARS2 with mito-location signal replaced by flag tag in mammalian cells, also carrying synonymous mutations described in Addgene #232340

    Depositor

    Quanfu Ma

    Insert

    IARS2 (IARS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-48 (indicated as Mito-signal in UniPro…

    Promoter

    CMV

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge