We narrowed to 9,202 results for: sacs

1. Hsh155 K740R

   Plasmid

   #111953

   Purpose

   This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740R mutation

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

   Insert

   Hsh155 K740R
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   K740R

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWS936

   Plasmid

   #123050

   Purpose

   G_ subunit for creating C-terminal transplants

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   Gpa1 C-terminal Truncation Vector [3-4a]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pESC-LEU2-RAD30cmyc

   Plasmid

   #60821

   Purpose

   to overexpress yeast polymerase eta in yeast

   Depositor

   Youri Pavlov, Thomas Kunkel

   Article

   Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

   Insert

   RAD30 (RAD30 Budding Yeast)
   

   Use

   Tags

   c-myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pRS406 ATG9-GFP S122D

   Plasmid

   #114667

   Purpose

   Expresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Feng et al Autophagy. 2016;12(4):648-58. doi: 10.10

   Insert

   ATG9
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   changed serine 122 to aspartic acid

   Promoter

   ATG9

   Available since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS424-TUB2 (1-428)

   Plasmid

   #60395

   Purpose

   Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   deleted 429 - end

   Promoter

   GAL

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pGRE NPD-GFP

   Plasmid

   #1197

   Depositor

   Susan Lindquist

   Article

   Patino et al Science 1996 Aug 2;273(5275):622-6.

   Insert

   N-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   fused N-terminal Sup35 (~1-253aa) to GFP

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. YIplac211-RPL13A-FKBPx4

   Plasmid

   #104474

   Purpose

   Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx4-tagged version.

   Depositor

   Benjamin Glick

   Article

   Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

   Insert

   RPL13Ap (RPL13A Budding Yeast)
   

   Use

   Tags

   FKBPx4-HA

   Expression

   Yeast

   Mutation

   includes the last 354 bp of RPL13A only

   Promoter

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS314-PRP16

   Plasmid

   #89971

   Purpose

   Prp16 in a trp backbone complements prp16 genomic deletion

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   none

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPS1358

   Plasmid

   #8918

   Depositor

   Pamela Silver

   Article

   Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

   Insert

   HRP1 (HRP1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. sup35-C653R

    Plasmid

    #1198

    Depositor

    Susan Lindquist

    Article

    True et al Nature 2004 Sep 9;431(7005):184-7. Epub

    Insert

    Sup35p (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed cys 653 to arg

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pAG416GPD-Cerulean-YPT52

    Plasmid

    #18843

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT52 (YPT52 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

12. POT2-CYC1p-ycrtE-ADH1t

    Plasmid

    #65354

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    CYC1p-ycrtE-ADH1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. POT4-CYC1p-ycrtI-ADH1t

    Plasmid

    #65357

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    CYC1p-ycrtI-ADH1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG416GPD-Cerulean-YPT1

    Plasmid

    #18848

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT1 (YPT1 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pJC20Cerulean3-Cdc8

    Plasmid

    #99357

    Purpose

    Bacterial expression of Cerulean3 fused to the 5' of cDNA of the fission yeast tropomyosin, cdc8.

    Depositor

    Dan Mulvihill

    Article

    Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Cerulean3 and His6-Gly3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJRL2 PHO5(TATA-A1T6)pr ECFP (EB#1560)

    Plasmid

    #14846

    Depositor

    Erin O'Shea

    Article

    Raser et al Science. 2004 Jun 18. 304(5678):1811-4.

    Insert

    PHO5 promoter TATA-A1T6 (PHO5 Budding Yeast)
    

    Use

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    TATA-A1T6. TATA box mutated from TATATAAG to AATA…

    Promoter

    Available since

    May 25, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pGST1-yGGA1-VHS

    Plasmid

    #44421

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    yGGA1-VHS (GGA1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

18. p413TEF-PYK2

    Plasmid

    #34606

    Depositor

    Markus Ralser

    Article

    Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

    Insert

    PYK2 (PYK2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAG416GPD-Cerulean-YPT6

    Plasmid

    #18845

    Depositor

    Susan Lindquist

    Article

    Gitler et al Proc Natl Acad Sci U S A. 2008 Jan 8. 10

    Insert

    YPT6 (YPT6 Budding Yeast)
    

    Use

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pJLI-Sup35?M

    Plasmid

    #1225

    Depositor

    Susan Lindquist

    Article

    Liu et al Proc Natl Acad Sci U S A 2002 Dec 10;99

    Insert

    SUP35 (SUP35 Budding Yeast)
    

    Use

    Yeast integrative plasmid

    Tags

    Expression

    Mutation

    deletion of aa 124-253 of SUP35

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. POT4-TEF2p-ycrtI-ADH1t

    Plasmid

    #65356

    Purpose

    TU for combinatorial construction of ?-carotene pathway

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    TEF2p-ycrtI-ADH1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAW8ENdeI-cyEGFP-spo5DSR

    Plasmid

    #49867

    Purpose

    for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

    Depositor

    Tony Carr

    Article

    Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    yEGFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. sup35-R320I

    Plasmid

    #1199

    Depositor

    Susan Lindquist

    Article

    True et al Nature 2004 Sep 9;431(7005):184-7. Epub

    Insert

    Sup35p (SUP35 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    changed arg 320 to isoleucine

    Promoter

    Available since

    May 25, 2005

    Availability

    Academic Institutions and Nonprofits only

24. pME-basGal80

    Plasmid

    #51621

    Purpose

    vertebrate expression of Gal80 preceded by a basal promoter

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80 (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    Expression

    Mutation

    Promoter

    basal promoter

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

25. M3939 leu2::ADE2 Disruptor Converter

    Plasmid

    #51672

    Purpose

    Yeast marker swap plasmid for converting LEU2 to ADE2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    ADE2 (ADE2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADE2

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDR-meptrac-H194E

    Plasmid

    #47764

    Purpose

    expresses MepTrac-H194E in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    MepTrac-H194E
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    H194E

    Promoter

    PMA

    Available since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFP125

    Plasmid

    #21674

    Depositor

    James Haber

    Article

    Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

    Insert

    LacZ (lacZ E. coli)
    

    Use

    Centromeric plasmid

    Tags

    Expression

    Mutation

    The second lacZ sequence has been completely dele…

    Promoter

    Available since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pJC45BS104 L892W

    Plasmid

    #1308

    Depositor

    Susan Lindquist

    Article

    Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

    Insert

    Hsp104 L892W (HSP104 Budding Yeast)
    

    Use

    Tags

    10X His tag

    Expression

    Bacterial

    Mutation

    Changed Leu 892 to Trp

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPS1597

    Plasmid

    #8920

    Depositor

    Pamela Silver

    Article

    Valentini et al Genetics. 2002 Feb . 160(2):393-405.

    Insert

    TIF51A (HYP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

30. pRS414-54

    Plasmid

    #23084

    Depositor

    Sharon Dent

    Article

    Tripic et al Biochem Biophys Res Commun. 2006 Jan 20.

    Insert

    yeast Histone H3-2 and Histone H4-2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Histone H3 changed Lysines 4 and 9 to Glutamines,…

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only