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We narrowed to 9,406 results for: Coli

Showing: 8401 - 8450 of 9406 results

  1. CRBN_ΔHBD

    Plasmid
    #232779
    Purpose
    Expresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domain
    Depositor
    Ivan Dikic
    Article
    Bailey et al Cell Chem Biol. 2024 Nov 20:S2451-9456(2
    Insert
    Cereblon (CRBN Human)
    Use
    Tags
    MBP-His6-TEV
    Expression
    Bacterial
    Mutation
    CRBN (residues 47 to 193 and 249 to 436) with GNG…
    Promoter
    T7
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232779-plasmid-map-sequence-id-462151

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  2. pET3a-SF-LMNC-TS

    Plasmid
    #169374
    Purpose
    E. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.
    Depositor
    Bernd Bukau
    Article
    Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi
    Insert
    LMNA residues 31-542 (LMNA Human)
    Use
    Tags
    Split-FlAsH Tag and TwinStrep Tag
    Expression
    Bacterial
    Mutation
    deleted amino acid 1-30 (unstructured head domain…
    Promoter
    Native pET3a promotor
    Available Since
    May 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    169374-plasmid-map-sequence-id-333772

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  3. t1-405(del37/GAG)

    Plasmid
    #166129
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Contains 37 amino acid deletion in F1-loop, replaced with Gly-Ala-Gly. N-terminal His6, Xpress-epitopeDLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del37/GAG) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405, with 37 aa deletion in the F1-loop which…
    Promoter
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166129-plasmid-map-sequence-id-330258

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  4. pMWCAVI_H6BAP-OROV_N

    Plasmid
    #240162
    Purpose
    Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli
    Depositor
    Stephen Graham
    Article
    Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/
    Insert
    Nucleoprotein
    Use
    Tags
    6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    240162-plasmid-map-sequence-id-479202

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  5. pGEX-5X-1-GST-Armadillo-delta4-6

    Plasmid
    #242226
    Purpose
    Expression of GST-tagged human β-catenin Armadillo repeats ∆4-6 without IDRs (deletion of a.a. 2-133, 268-394, and 672-781) in E. coli
    Depositor
    Courtney Hodges
    Article
    Chan et al Mol Cell. 2025 Jul 15:S1097-2765(25)0057
    Insert
    CTNNB1 (CTNNB1 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Deleted N terminal IDR, arm 4-6, and C terminal I…
    Promoter
    tac
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    242226-plasmid-map-sequence-id-481884

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  6. pHis-TEV-Nsp15-E266Q

    Plasmid
    #218803
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    E266Q (G to C)
    Promoter
    Available Since
    May 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    218803-plasmid-map-sequence-id-433302

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  7. pHis-TEV-Nsp15-V66L

    Plasmid
    #219524
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    V66L (G to C)
    Promoter
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219524-plasmid-map-sequence-id-433377

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  8. pHis-TEV-Nsp15-A81V

    Plasmid
    #219525
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    A81V (C to T)
    Promoter
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219525-plasmid-map-sequence-id-433375

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  9. pHis-TEV-Nsp15-D183N

    Plasmid
    #219526
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    D183N (G to A)
    Promoter
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219526-plasmid-map-sequence-id-433305

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  10. pHis-TEV-Nsp15-H234A

    Plasmid
    #219529
    Purpose
    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag
    Depositor
    Debra Hansen
    Article
    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:
    Insert
    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    TEV protease cleavable His6-tag
    Expression
    Bacterial
    Mutation
    H234A (CAC to GCG)
    Promoter
    Available Since
    May 27, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    219529-plasmid-map-sequence-id-433314

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  11. t1-405(del30)

    Plasmid
    #166128
    Purpose
    Expression of human talin-1 head (residues 1-405) in E. coli. Contains 30 amino acid deletion in F1-loop. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(del30) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, with 30 amino acid deletion in th…
    Promoter
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166128-plasmid-map-sequence-id-330260

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  12. t1-405(T144E,T150E)

    Plasmid
    #166130
    Purpose
    For expression of human talin-1 head (residues 1-405) in E. coli. Includes mutations T144E and T150E. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    HuT1head1-405(T144E,T150E) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-405, mutations T144E and T150E
    Promoter
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166130-plasmid-map-sequence-id-330250

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  14. pOPINB-M1-di-Ub(G76V)-HOIL-1

    Plasmid
    #229539
    Purpose
    Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Life Sci Alliance. 2025 Apr 1;8(6):e2025
    Inserts
    Ubiquitin (UBC Human)
    Ubiquitin (UBC Human)
    HOIL-1 (RBCK1 Human)
    Use
    Tags
    6xHis-3C
    Expression
    Bacterial
    Mutation
    Changed Gly 76 to Val and changed Gly 76 to Val
    Promoter
    T7
    Available Since
    Dec. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    229539-plasmid-map-sequence-id-456666

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  24. t1-405(151-154AAAA)

    Plasmid
    #166131
    Purpose
    Expression of human talin-1 head (aa1-405) in E. coli. Four substitutions in the F1-loop (aa151-154 all mutated to Al). N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site.
    Depositor
    Vesa Hytönen
    Article
    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13
    Insert
    T1head1-405(151-154AAAA) (TLN1 Human)
    Use
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    aa1-405 only. Contains 4 amino acid substitutions…
    Promoter
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166131-plasmid-map-sequence-id-330282

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Showing: 8401 - 8450 of 9406 results