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We narrowed to 12,214 results for: LEA

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Showing: 3361 - 3380 of 12214 results

  2. BB44_pmc.DonorR5.TS

    Plasmid
    #199223
    Purpose
    Donor plasmid with CCR5 target sites for ITPN or HMEJ knock-in at the human CCR5 safe harbour locus
    Depositor
    Manuel Goncalves
    Publication
    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Expression unit for mCherry - monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)
    Use
    Gene targeting donor plasmid
    Expression
    Mammalian
    Promoter
    Human PGK1 gene promoter
    Available since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199223-plasmid-map-sequence-id-391672

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  7. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid
    #89869
    Purpose
    expresses iTango bArrestin2-TevC component and TdTomato bicistronically
    Depositor
    Hyungbae Kwon
    Publication
    Lee et al Nat Methods. 2017 May;14(5):495-503. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bArrestin2-TevC-P2A-TdTomato
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    R173H in TdTomato (please see depositor comments …
    Promoter
    CMV
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    89869-plasmid-map-sequence-id-177073

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  9. EBFP2-PCNA-19-SV40NLS-4

    Plasmid
    #55250
    Purpose
    Localization: Nucleus, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Tags
    3xSV40 NLS and EBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    55250-plasmid-map-sequence-id-230171

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  14. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  15. MSP2372

    Plasmid
    #73196
    Purpose
    Human expression plasmid for SpCas9(R661A/Q695A/Q926A) variant: CMV-T7-humanSpCas9(R661A, Q695A, Q926A)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (R661A/Q695A/Q926A)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, Q695A, and Q926A in SpCas9
    Promoter
    CMV
    Available since
    Feb. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73196-plasmid-map-sequence-id-134659

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  16. pcDNA-NES-ZapCY2

    Plasmid
    #59016
    Purpose
    Cytosolic zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor
    Tags
    Nuclear Exclusion Signal
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 3361 - 3380 of 12214 results