Addgene

We narrowed to 9,995 results for: AGA

Showing: 801 - 850 of 9995 results

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pEF/myc/ER FLIPglu-600uDelta13V

Plasmid

#18020

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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SCP1-pUC119

Plasmid

#21846

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP1 (super core promoter 1)-pUC119

Use

Puc derivative - a cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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PA-TNXL/pcDNA3

Plasmid

#51994

Purpose

mammalian expression of a 'caged' Ca2+ indicator for fluorescent imaging at specific locations and time points

Depositor

Takeharu Nagai

Article

Matsuda et al Sci Rep. 2013;3:1398. doi: 10.1038/srep0

Insert

PA-TNXL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pEF/myc/ER FLIPglu-30uDelta13V

Plasmid

#18021

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu D154A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

mglB-D154A

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pEF/myc/ER FLIPglu-170nDelta13V

Plasmid

#18022

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16F Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

WT binding protein (no F16A)

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pHSG-PCNA1

Plasmid

#66125

Purpose

S. solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

Insert

DNA polymerase sliding clamp 1 (SSO_RS01995 Sulfolobus solfataricus P2)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pHSG-PCNA3

Plasmid

#66127

Purpose

S. solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

Insert

DNA polymerase sliding clamp 3 (pcn Sulfolobus solfataricus P2)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pHSG-PCNA2

Plasmid

#66126

Purpose

S. solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

Insert

DNA polymerase sliding clamp 2 (SSO_RS05185 Sulfolobus solfataricus P2)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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SCP2-pUC119

Plasmid

#21951

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP2 (super core promoter 2)-pUC119

Use

A puc derivative - a cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pXeX-SHH

Plasmid

#31233

Depositor

Cliff Ragsdale

Article

Agarwala et al Science. 2001 Mar 16. 291(5511):2147-50.

Insert

SHH (SHH Chicken)

Use

Xenopus expression vector

Tags

Expression

Mutation

starts at aa4

Promoter

Available since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pmPlum-histone H3

Plasmid

#179700

Purpose

visualization of nucleus/chromosome

Depositor

Kenji Sugimoto

Article

Nakagawa et al Mol Biol 2016, 5:2

Insert

Histone H3 (Hist1h3h Mouse)

Use

Tags

mPlum

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBsaGSPdR

Plasmid

#108205

Purpose

PdR

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Putidaredoxin reductase

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac promoter

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHPro54G

Plasmid

#108204

Purpose

4th Pro5

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Pro-Pro-Pro-Pro-Pro

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac promoter

Available since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
siCheck2-6xmiR307_23nt-nearperfect

Plasmid

#41099

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

6xmiR307_23nt-nearperfect

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
siCheck2-8xmiR307_23nt-seedonly

Plasmid

#41102

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

8xmiR307_23nt-seedonly

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-8xmiR307_21nt-seedonly

Plasmid

#41103

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

8xmiR307_21nt-seedonly

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-4xmiR307_21nt-seedonly

Plasmid

#41101

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

4xmiR307_21nt-seedonly

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-4xmiR307_23nt-seedonly

Plasmid

#41100

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

4xmiR307_23nt-seedonly

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-6xmiR307_21nt-nearperfect

Plasmid

#41098

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

6xmiR307_21nt-nearperfect

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-taranismutant3'UTR

Plasmid

#41107

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

taranismutant3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-taranis3'UTR

Plasmid

#41106

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

taranis3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-Gkmutant3'UTR

Plasmid

#41105

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Gk3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
siCheck2-Gk3'UTR

Plasmid

#41104

Depositor

Phillip Zamore

Article

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

Gk3'UTR

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only
pCMV mouse sidekick2

Plasmid

#18735

Depositor

Joshua Sanes

Article

Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

Insert

Sidekick-2 (Sdk2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only
pCMV mouse sidekick1

Plasmid

#18734

Depositor

Joshua Sanes

Article

Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

Insert

Sidekick-1 (Sdk1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only
Sumire-pRsetB

Plasmid

#193361

Purpose

GFP variant emit 414 nm fluorescence.

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

Sumire

Use

E.coli expression

Tags

His X6 tag

Expression

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mVenus-pBAD

Plasmid

#54845

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Use

Tags

6xHis and mVenus

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Article

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Insert

Gamillus

Use

Tags

No tag

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Sirius/pcDNA3

Plasmid

#51957

Purpose

mammalian expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent protein

Depositor

Takeharu Nagai

Article

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Insert

Sirius

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyCA

Plasmid

#84268

Purpose

A versatile vector for recombinant mycobacterial protein production

Depositor

Young-Hwa Song

Article

Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Aceteamidase

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24-ISWI-iCBD

Plasmid

#105499

Purpose

Expression of Drosophila ISWI C-terminally tagged with Combined Sce VMA intein and chitin binding domain (iCBD)

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

ISWI-iCBD

Use

Tags

Expression

Mutation

Promoter

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pESC-URA-Rnq1-GFP

Plasmid

#21051

Depositor

Judith Frydman

Article

Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

Insert

Rnq1 (RNQ1 Budding Yeast)

Use

Yeast/bacteria shuttle

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pET11a-dH2B

Plasmid

#105501

Purpose

Expression of Drosophila histone H2B. An additional Ile codon was inserted after the initiating Met codon to enhance the protein expression level

Depositor

James Kadonaga

Article

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

dH2B

Use

Tags

Expression

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-U6-EMX1

Plasmid

#140581

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA EMX1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pM1GG

Plasmid

#108200

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA1

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF/myc/ER FLIPglu-3.2mDelta13V

Plasmid

#18019

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_AC_p45582

Plasmid

#154036

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter 45582

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_EF_tNub

Plasmid

#154039

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator Nub

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_AC_pSVP

Plasmid

#154040

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter SVP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_EF_tPbt

Plasmid

#154043

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator Pbt

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT016l

Plasmid

#96857

Purpose

(d)theophylline-agRNA-GFP

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)theophylline-agRNA-GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Phamret/pcDNA3

Plasmid

#51953

Purpose

mammalian expression of Phamret, a monomeric photoconvertible fluorescent protein.

Depositor

Takeharu Nagai

Article

Matsuda et al Nat Methods. 2008 Apr;5(4):339-45. doi:

Insert

Phamret

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only
pPD2X

Plasmid

#86967

Purpose

Expresses PCNA2 protein fused phosphite dehydrogenase and putidaredoxin in E. coli

Depositor

Teruyuki Nagamune

Article

Watanabe et al ChemCatChem 2013 Dec;5(12):3835-3840

Insert

PCNA2 fused to phosphite dehydrogenase and putidaredoxin

Use

Tags

His6 tag

Expression

Bacterial

Mutation

E175A/A176R in PTDH, C73S/C85S in PdX

Promoter

T7 promoter

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pWT055b

Plasmid

#96877

Purpose

theophylline-agRNA-FANCF

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

theophylline-agRNA-FANCF

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IB-FKBP-HaloTag

Plasmid

#233350

Purpose

Stable expression of FKBP-Halotag in mammalian cells. FKBP-halotag localizes in the cytosol and binds to the FRB tag in the presence of AP21967 (A/C Heterodimerizer).

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Insert

FKBP

Use

Retroviral

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDual_dsCas9_Puro_hs_NC_chr1

Plasmid

#214688

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-Puromycin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

Depositor

Kevin Janes

Insert

dgRNA_Chr1

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Puromycin resistance cassette has silent mutation…

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pED1a6

Plasmid

#134787

Purpose

Luciferase reporter for quadruplet codon TAGA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

luxAB-357-TAGA

Use

Luciferase and Synthetic Biology

Tags

Expression

Bacterial

Mutation

357-TAGA

Promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_EF_t45582

Plasmid

#154037

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator 45582

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_AC_pNub

Plasmid

#154038

Purpose

Diatom promoter for P. tricornutum; uLoop specific syntax; AC overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Promoter Nub

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_EF_tSVP

Plasmid

#154041

Purpose

Diatom terminator for P. tricornutum; uLoop specific syntax; EF overhangs; Lvl 0

Depositor

Raffaela Abbriano

Article

Windhagauer et al Algal Research , Volume 53, March 2021

Insert

Terminator SVP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 801 - 850 of 9995 results

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