We narrowed to 14,014 results for: TIM

1. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized

   Plasmid

   #231602

   Purpose

   Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized under CMV promoter

   Depositor

   Marvin Tanenbaum

   Article

   Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

   Insert

   Tornado-1xALFATag-5xSunTag_codon_optimized
   

   Expression

   Mammalian

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

   Plasmid

   #163991

   Purpose

   Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 CARD (NLRP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD649-HAsp-TIMD4-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156731

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   TIMD4 (TIMD4 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-codon optimized catalytically inactive PHGDH

   Plasmid

   #154903

   Purpose

   Expresses codon optimized catalytically inactive PHGDH in mammalian cells

   Depositor

   Michael Pacold

   Article

   Ngo et al Cancer Discov. 2020 Sep;10(9):1352-1373.

   Insert

   Phosphoglycerate dehydrogenase (PHGDH Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Three mutations: D175N, R236K, H283A

   Promoter

   TRE

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZ005 (AacC2c1 human codon optimized protein)

   Plasmid

   #70171

   Purpose

   Expresses AacC2c1 human codon optimized protein

   Depositor

   Feng Zhang

   Article

   Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

   Insert

   AacC2c1 protein
   

   Tags

   NLS

   Available Since

   May 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTR-339 pUC19-tNGFR-P2A-TIM3 N

   Plasmid

   #186077

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   TIM3 (HAVCR2 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV hSyn INTRON HaloTag-STIM1 WPRE

   Plasmid

   #236233

   Purpose

   AAV expression of mouse STIM1 internally tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   HaloTag-Stim1 (Stim1 Mouse)
   

   Use

   AAV

   Tags

   HaloTag

   Promoter

   human Synapsin 1

   Available Since

   May 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV hSyn INTRON mScarlet-STIM2 WPRE

   Plasmid

   #236236

   Purpose

   AAV expression of human STIM2 internally tagged with mScarlet-I

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mScarletI-STIM2 (STIM2 Human)
   

   Use

   AAV

   Tags

   mScarletI in position 29

   Mutation

   Signal peptide from STIM1 and residues 15-746 of …

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV hSyn INTRON mEmerald-STIM1 WPRE

   Plasmid

   #236234

   Purpose

   AAV expression of mouse STIM1 internally tagged with mEmerald

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

   Insert

   mEmerald-Stim1 (Stim1 Mouse)
   

   Use

   AAV

   Tags

   mEmerald

   Promoter

   human Synapsin 1

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV hSyn INTRON HaloTag-STIM2 WPRE

    Plasmid

    #236235

    Purpose

    AAV expression of human STIM2 internally tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    HaloTag-STIM2 (STIM2 Human)
    

    Use

    AAV

    Tags

    HaloTag in position 29

    Mutation

    Signal peptide from STIM1 and residues 15-746 of …

    Promoter

    human Synapsin 1

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-SpCas9(RRTIMR)-P2A-EGFP (AHK149)

    Plasmid

    #223071

    Purpose

    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RRTIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(RRTIMR)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpCas9(RRTIMR)=D1135R, S1136R, G1218T, E1219I, R1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

    Plasmid

    #221112

    Purpose

    FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    MFA-IgKappa-FAPoptim-STE3
    

    Expression

    Yeast

    Promoter

    STE3

    Available Since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD649-HAsp-TIMD4-COMP5AP-AviTag-9xHis

    Plasmid

    #157295

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    TIMD4 (TIMD4 Human)
    

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

    Plasmid

    #184563

    Purpose

    CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

    Depositor

    Kacper Rogala

    Article

    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

    Insert

    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    All lysines were mutated to arginines. Codon-opti…

    Promoter

    CMV

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

    Plasmid

    #205631

    Purpose

    Expression of TnpBmax in mammalian cells

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    ISDra2-TnpB optimized for mammalian expression
    

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRetroX-Tight-Pur-Trim69 isoform B (codon optimized)

    Plasmid

    #199567

    Purpose

    Stable and dox. inducible expression of Trim69 isoform B (codon optimized) after retroviral-mediated gene transfer

    Depositor

    Andrea Cimarelli, Yuxin Song

    Article

    Song et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

    Insert

    Trim69 isoform B (codon optimized) (TRIM69 Human)
    

    Use

    Retroviral; Allows for puromycin selection

    Tags

    Flag

    Mutation

    codon optimized

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-G1B3-Mito-Timer-WPRE-miR124T-SYNpolyA

    Plasmid

    #245076

    Purpose

    Mito-Timer fluorescent reporter, with miRNA-124 target sequence to repress transgene expression in neurons

    Depositor

    Nicole Déglon

    Insert

    mito-Timer
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human G1B3 promoter

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)

    Plasmid

    #231603

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-4x(6xAAA) under CMV promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag_codon_optimized-4x(6xAAA)
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV-CMVp-2xTetO-Tornado-1xALFATag-5xSunTag_codon_optimized+8xAAA

    Plasmid

    #231604

    Purpose

    Express circular RNA translation reporter with 1xALFATag-5xSunTag_codon_optimized-8xAAA under CMV promoter

    Depositor

    Marvin Tanenbaum

    Article

    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

    Insert

    Tornado-1xALFATag-5xSunTag_codon_optimized-8xAAA
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid

    #225939

    Purpose

    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva, Evan Reid

    Inserts

    STIM1 (STIM1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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