We narrowed to 34,448 results for: CaS

1. mCerulean3-EB3-C-20

   Plasmid

   #55417

   Purpose

   Localization: MT End Binding Protein, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   EB3 (MAPRE3 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pET28_6xHis-PARP1-A762V

   Plasmid

   #174795

   Purpose

   Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A762V (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A762V

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMXs_FLAG-FSF1

   Plasmid

   #110641

   Purpose

   Retroviral expression of flag-tagged yeast FSF1 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   FSF1 (FSF1 Budding Yeast)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDNA3-HA/Bmal L95E

   Plasmid

   #47344

   Purpose

   PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   BMAL (Bmal1 Mouse)
   

   Use

   Tags

   Ha

   Expression

   Mammalian

   Mutation

   L95E

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pJMP1274

   Plasmid

   #119266

   Purpose

   B. subtilis deltaTn7 site and flanking sequence cloned into pACYC

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   B. subtilis deltaTn7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJMP1263

   Plasmid

   #119264

   Purpose

   B. subtilis attTn7 site and flanking sequence cloned into pACYC

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   B. subtilis attTn7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Rat SERCA2b (pMT2)

   Plasmid

   #75184

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   Jonathan Lytton, Gary Shull

   Article

   Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

   Insert

   ATP2A2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   adenovirus major late promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CRY-tTA (B702)

   Plasmid

   #92032

   Purpose

   Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

   Depositor

   Chandra Tucker

   Article

   Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   tTA2 (TetR fused to VP64)

   Expression

   Mammalian

   Mutation

   NLS on CRY2 is mutated

   Promoter

   CMV

   Available since

   July 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXylan

   Plasmid

   #36301

   Depositor

   Jay Keasling

   Article

   Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

   Insert

   pXylan
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Two promoters from the E coli genome drive two di…

   Available since

   Nov. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. dmBACCS2VL-IRES-dOrai-IRES-GFP

    Plasmid

    #72897

    Purpose

    Mammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFP

    Depositor

    Takao Nakata

    Article

    Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

    Insert

    dmBACCS2VL-IRES-dOrai-IRES-GFP
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LB1461 (Bcl-2 promoter with MHS1234, NFkB mut)

    Plasmid

    #15385

    Depositor

    Linda Boxer

    Article

    Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

    Insert

    B-cell lymphoma/leukemia-2 (BCL2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Bacterial and Mammalian

    Mutation

    Bcl-2 full promoter with IgH full enhancer MHS123…

    Promoter

    Available since

    Aug. 1, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pMXs_FLAG-ODC2

    Plasmid

    #133257

    Purpose

    Express FLAG-tagged ODC2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    ODC2 (ODC2 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs_FLAG-NDT2

    Plasmid

    #133255

    Purpose

    Express FLAG-tagged NDT2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT2 (YEA6 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-FLJ23445

    Plasmid

    #15429

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    FLJ23445 (Thoc7 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCMV10/3xflag-Clock L57E/L113E/W284A

    Plasmid

    #47343

    Purpose

    triple mutation used for co-IP

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L57E/L113E/W284A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3-HA/Bmal I317D

    Plasmid

    #47347

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    I317D

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEF Doc2beta 6X mutant GFP

    Plasmid

    #128823

    Purpose

    To visualize Doc2beta 6X mutant in PC12 cells

    Depositor

    Edwin Chapman

    Article

    Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

    Insert

    Doc2beta (Doc2b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D163A, D218A, D220N, D303A, D357A, D359A

    Promoter

    EF-1alpha

    Available since

    Nov. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3-HA/Bmal L115E

    Plasmid

    #47345

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L115E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

19. mCerulean3-EB3-7

    Plasmid

    #55416

    Purpose

    Localization: MT End Binding Protein, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-HA/Bmal W427A

    Plasmid

    #47349

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    W427A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

21. mCerulean3-Actin-C-18

    Plasmid

    #55400

    Purpose

    Localization: Actin, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pCDNA3-HA/Bmal V435R

    Plasmid

    #47350

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    V435R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

23. HOXB5 (human) pBluescript

    Plasmid

    #8531

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB5 (HOXB5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    makes protein in vitro transcription/translation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pCDNA3-HA/Bmal L150E

    Plasmid

    #47346

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    L150E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pLV-EF1A-PARP14-1535-1601-Hygro

    Plasmid

    #238100

    Purpose

    Expression of structurally predicted WWE domain of PARP14 with EGFP on C terminal

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    PARP14 (PARP14 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa 1535-1601 only

    Promoter

    EF1alpha

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-Hygro-EF1A-TRIP12(749-836)-EGFP

    Plasmid

    #196229

    Purpose

    EGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    C-terminal EGFP tagged TRIP12(749- 836)
    

    Use

    Lentiviral

    Tags

    EGFP and Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-Hygro-EF1A-DTX2(8-97)-EGFP

    Plasmid

    #196230

    Purpose

    EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    C-terminal EGFP tagged DTX2(8-97)
    

    Use

    Lentiviral

    Tags

    EGFP and Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV-Hygro-EF1A-DTX2(8-174)-EGFP

    Plasmid

    #196232

    Purpose

    EGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    DTX2(8-174)-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP and Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP

    Plasmid

    #196235

    Purpose

    EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    C-terminal EGFP tagged Af1521(K35E/Y145R)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K35E and Y145R

    Promoter

    EF1A

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-Hygro-EF1A-RNF146(100-182)-myc

    Plasmid

    #196236

    Purpose

    RNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    RNF146(100-182) encoding a C-terminal myc tag
    

    Use

    Lentiviral

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLV-Hygro-EF1A-RNF146(92-168)-EGFP

    Plasmid

    #196228

    Purpose

    EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

    Insert

    RNF146(92-168)-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP and Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    Aug. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Gab2-MSCV-IRES-GFP

    Plasmid

    #240126

    Purpose

    Expresses Gab2 (mouse) in mammalian cells

    Depositor

    Timothy Ley

    Article

    Kramer et al J Clin Invest. 2025 Aug 7:e195929. doi:

    Insert

    Gab2 (Gab2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAG-48xS100A10 array

    Plasmid

    #236381

    Purpose

    Expression of array of 48 crRNAs targeting S100A10

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    48xS100A10 crRNA array (S100A10 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHR TRE_TGFbeta3_IRES_mCherry-PGK_BFP

    Plasmid

    #223548

    Purpose

    Inducible TGF beta 3 and mCherry; constitutive BFP

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Inserts

    TGF beta 3 (TGFB3 Human)
    mCherry
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCS2+ 3xFLAG-FEM1B C186S

    Plasmid

    #230995

    Purpose

    Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Insert

    FEM1B (Fem1b Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    mutated Cys186 to Ser

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCS2+ 3xFLAG-FEM1B L597A

    Plasmid

    #230994

    Purpose

    Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Insert

    FEM1B (Fem1b Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    mutated Leu597 to Ala

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. NL4-3Δ6-drEGFP-IRES-mThy1.2

    Plasmid

    #207881

    Purpose

    HIV reporter vector expressing dGFP and mThy1.2 from Env locus

    Depositor

    Edward Browne

    Article

    Peterson et al Cell Chem Biol. 2023 Dec 21;30(12):1617-

    Insert

    destabilized EGFP-IRES-mThy1.2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    5' lentiviral LTR

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRGEB32-CrActin-35S

    Plasmid

    #226708

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from CaMV 35S promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrActin
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRGEB32-CrActin-UBI

    Plasmid

    #226709

    Purpose

    For CRISPR/Cas-mediated gene editing in model fern species, Ceratopteris richardii; expresses guide RNAs from CrActin promoter and Cas9 from maize ubiquitin (ZmUbi) promoter.

    Depositor

    Fenglin Deng

    Article

    Jiang et al Plant Physiol. 2024 Sep 12:kiae473. doi:

    Insert

    CrActin
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCHC025 (∆CBBp)

    Plasmid

    #226203

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator megaplasmid copy of the CBB operon (deltaCBBp).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for megaplasmid copy of the CBB operonthe CBB operon (deltaCBBp).
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆cbbR ∆cbbLpSpXpYpEpFpPpTpZpGpKpAp

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pCHC024 (∆SH)

    Plasmid

    #226202

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator soluble hydrogenase operon (deltaSH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for soluble hydrogenase operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆hoxFUYHWI ∆hypA2B2F2

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. pCHC023 (∆MBH)

    Plasmid

    #226201

    Purpose

    Electroporation knockout plasmid (pK18sB) to delete the C. necator membrane-bound hydrogenase operon (deltaMBH).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for membrane bound hydrogenase operon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    ∆hoxKGZMLOQRTV ∆hypA1B1F1CDEX ∆hoxABCJ

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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43. pCHC027 (∆pemK)

    Plasmid

    #226204

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator addiction system toxin PemK, to enable deletion of the pHG1 megaplasmid.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Upstream/downstream homology arms for addiction system toxinPemK.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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44. Q54A

    Plasmid

    #217912

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with the Q54A coil mutation

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP Q54A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Glutamine at position 55 and 267 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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45. T40A

    Plasmid

    #217911

    Purpose

    A plasmid encoding the hydrogel-forming protein PXP with the T40A coil mutation

    Depositor

    Cole DeForest

    Article

    Bennett et al Adv Sci (Weinh). 2024 Mar 13:e2301708. d

    Insert

    PXP T40A
    

    Use

    Tags

    6x His (N and C term)

    Expression

    Bacterial

    Mutation

    Threonine at position 41 and 253 mutated to Alani…

    Promoter

    T5

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSAVED-CHAT

    Plasmid

    #214041

    Purpose

    Bacterial expression plasmid for SAVED-CHAT from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. AA436

    Plasmid

    #216035

    Purpose

    Fragmid fragment: (Cas protein) binds MS2 stem loops

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    MS2_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. AA440

    Plasmid

    #216036

    Purpose

    Fragmid fragment: (Cas protein) binds to TRE in absence of doxycycline

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    TetR_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. AA201

    Plasmid

    #216026

    Purpose

    Fragmid fragment: (Cas protein) firefly luciferase

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Fluc_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. AA156

    Plasmid

    #216022

    Purpose

    Fragmid fragment: (Cas protein) Nanobody binds ALFA tag

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    NbALFA_v1.1
    

    Use

    CRISPR; Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge