Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 11,594 results for: Emb;

Clear keywords
Showing: 3381 - 3400 of 11594 results

  1. hsp70l: hAPOB48-mCherry.

    Plasmid
    #140639
    Purpose
    Heat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.
    Depositor
    Steven Farber
    Article
    Walsh et al J Lipid Res. 2019 Dec 30. pii: jlr.RA119
    Inserts
    hsp70l (hsp70l Zebrafish)
    APOB (APOB Human)
    Use
    Zebrafish expression
    Tags
    mCherry
    Mutation
    APOB48: amino acid 1-2180
    Promoter
    hsp70l
    Available Since
    Dec. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140639-plasmid-map-sequence-id-308799

    Enlarge

  3. pBBA57-TEV-His12

    Plasmid
    #137033
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length BBA57, contains signal pep…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137033-plasmid-map-sequence-id-275688

    Enlarge

  9. pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk

    Plasmid
    #131143
    Purpose
    Rescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Insert
    mNeonGreen::3XFLAG::Crk (Crk Fly)
    Use
    Cre/Lox; Attb recombination site
    Tags
    3XFLAG and mNeonGreen
    Expression
    Insect
    Available Since
    Oct. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    131143-plasmid-map-sequence-id-258814

    Enlarge

  14. pLV-EF1A-CD19.8H.28TM.28.BB.3z

    Plasmid
    #200676
    Purpose
    Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200676-plasmid-map-sequence-id-391908

    Enlarge

  15. pLV-EF1A-CD19.8H.28TM.BB.28.3z

    Plasmid
    #200677
    Purpose
    Modular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200677-plasmid-map-sequence-id-391894

    Enlarge

  16. Anti-Halobacterium GvpA scFv 2B10-1-pcDNA 3.4 TOPO

    Plasmid
    #174688
    Purpose
    Plasmid for recombinant expression in mammalian cells of an anti-Halobacterium salinarum GvpA single chain variable fragment derived from monoclonal antibody 2B10-1
    Depositor
    Jean Gariepy
    Article
    Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6
    Insert
    Anti- Halobacterium salinarum GvpA single chain variable fragment 2B10-1
    Tags
    hemagglutinin (HA) tag, hexahistidine (HIS) tag
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV promoter
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174688-plasmid-map-sequence-id-347064

    Enlarge

  17. pZE21/UBP1/ClpS

    Plasmid
    #98566
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon
    Depositor
    George Church
    Article
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    In a synthetic operon downstream of UBP1 and Remo…
    Promoter
    Same as UBP1 (operon) and Tet promoter (aTc induc…
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98566-plasmid-map-sequence-id-203067

    Enlarge

  18. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid
    #187844
    Purpose
    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Article
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…
    Expression
    Mammalian
    Mutation
    meGFPe: asparagine 198 to aspartic acid and tyros…
    Promoter
    CMV
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187844-plasmid-map-sequence-id-379371

    Enlarge

  20. pCAG-VHC

    Plasmid
    #112618
    Purpose
    expression plasmid with CAG promoter driving multicistronic cassette H2BVenus_p2A_HygroR_p2A_CreERt2
    Depositor
    Stefan Heller
    Article
    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18
    Inserts
    H2B-Venus (H2BC3 Human, Aequorea victoria)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    Tags
    Venus
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112618-plasmid-map-sequence-id-222032

    Enlarge

Showing: 3381 - 3400 of 11594 results