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6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_C1

Plasmid

#85044

Purpose

Mammalian expression of mScarlet-i

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

mScarlet-i

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCW-Cas9-2A-EGFP

Plasmid

#167928

Purpose

Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

T2A-EGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

tTRE, hPGK

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pOZITX-S1

Plasmid

#184925

Purpose

Expresses the catalytic S1 subunit of OZITX (EcPltAB)

Depositor

Jonathan Javitch

Article

Keen et al Commun Biol. 2022 Mar 23;5(1):256. doi:

Insert

codon optimized S1 subunit of OZITX

Expression

Mammalian

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI Probe

Plasmid

#211507

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BcPI-PLC-ANH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-puro_UCOE_TRE3VG_mCherry-Responder

Plasmid

#230053

Purpose

It presents the UCOE sequence and the TRE3VG inducible promoter allowing a more stable dox-dependent inducible activation of mCherry though the OPTi-OX platform across differentiation of hiPSCs

Depositor

Alessandro Bertero

Article

Guichardaz et al Open Res Eur. 2024 Dec 18;4:266. doi: 10

Insert

UCOE

Expression

Mammalian

Promoter

TRE3VG

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pSIN4-LSM14A

Plasmid

#240022

Purpose

Expresses human LSM14A fused to 3X Flag in mammalian cells

Depositor

Rui Su

Article

Bi et al Nat Commun. 2025 Dec 2;17(1):260. doi: 1

Insert

LSM14A

Use

Lentiviral

Tags

3X Flag

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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JC201: pMVP/Ad/5-35-DEST

Plasmid

#121847

Purpose

pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGABASnFR

Plasmid

#112172

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

AAV

Promoter

GFAP

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4/5FTrp)RS

Plasmid

#207620

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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IX001: pBS/Fiber

Plasmid

#131754

Purpose

Plasmid containing Ad5 Fiber gene flanked by homology arms for insertion into the recombinant Ad5 vector IQ013 to generate tropism modified vectors.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Ad5 wild-type Fiber

Use

Synthetic Biology

Promoter

N/A

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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TEV Protease-mCherry

Plasmid

#243793

Purpose

Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TEV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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nls-transposase-nls-pA in pT3TS-Dest

Plasmid

#140873

Purpose

Vector for in vitro transcription of Tol2 nls-transposase-nls for zebrafish transgenesis using T3.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

nls-transposase-nls (Tol2)

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pB-EF1a-NLuc-IRES-Puro

Plasmid

#130936

Purpose

PiggyBac vector for constitutive NanoLuc expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NanoLuc

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pEIGW roGFP2-Orp1

Plasmid

#64993

Purpose

Mammalian expression of cytosolic roGFP2-Orp1 (lentiviral vector)

Depositor

Tobias Dick

Article

Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

Insert

Orp1 (HYR1 Budding Yeast)

Use

Lentiviral

Tags

roGFP2

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR1A-3XFlag-WT-MEFV

Plasmid

#167018

Purpose

Gateway entry plasmid with 3xflag-pyrin (Wild-type)

Depositor

Thomas Henry

Article

Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

Insert

MEFV (MEFV Human)

Use

Gateway Cloning

Tags

3xFlag

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta K85A pcDNA3

Plasmid

#14755

Depositor

Jim Woodgett

Article

Hughes et al EMBO J. 1993 Feb . 12(2):803-8.

Insert

GSK3 beta K85A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

K85A. Kinase dead mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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pSpCas9 (PX165)

Plasmid

#48137

Purpose

Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Ii-OVA.I+II

Plasmid

#64596

Purpose

Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

Depositor

Sandra Diebold, Martin Zenke

Article

Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

Insert

Ovalbumin

Tags

Invariant chain

Expression

Mammalian

Mutation

Only amino acids 242-353 (class I and class II ep…

Promoter

CMV

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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