We narrowed to 17,776 results for: REV

1. CPH3509 (YCp LEU2 Rpb1 T1471A)

   Plasmid

   #91812

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed threonine 1471 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3511 (YCp LEU2 Rpb1 P1472A)

   Plasmid

   #91813

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed proline 1472 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3567 (pET151_Spt6 1247-1451 R1282H)

   Plasmid

   #91816

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed arginine 1282 to histidine; deleted resid…

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CPH3495 (pET151_Spt6 1247-1451 K1435A)

   Plasmid

   #91817

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1435 to alanine; deleted residues …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKdH_P-GAP-ScACS1*

   Plasmid

   #126736

   Purpose

   yeast plasmid overexpressing ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acs1*
   

   Use

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C and A30S, P113S, and S629N (please see dep…

   Promoter

   GAP

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKdH_P-GAP-ScADH2+P-GAP*-ScACS1*

   Plasmid

   #126738

   Purpose

   yeast plasmid overexpressing ADH2 and ACS1 from Saccharomyces cerevisiae

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   adh2,acs1*
   

   Use

   Tags

   6xHis,Myc

   Expression

   Yeast

   Mutation

   T3542C

   Promoter

   GAP

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET151-Vps4(101-437)-Hcp1 K321D

   Plasmid

   #89288

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Lys-321 to Asp

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET151-Vps4(101-437)-Hcp1 R325D

   Plasmid

   #89289

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Arg-325 to Asp

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET151-Vps4(101-437)-Hcp1 E322A

   Plasmid

   #87748

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Glu-322 to Ala

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET151-Vps4(101-437)-Hcp1 R325A

    Plasmid

    #87749

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET151-Vps4(101-437)-Hcp1 K321A

    Plasmid

    #87747

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p3HAT1Luc1

    Plasmid

    #236275

    Purpose

    Mammalian expression of human angiotensin II type 1 receptor; N-terminal HA tag, C-terminal Rluc8

    Depositor

    Vsevolod Gurevich

    Article

    Gimenez et al J Biol Chem. 2012 Aug 24;287(35):29495-5

    Insert

    angiotensin II type 1 receptor (AGTR1 Human)
    

    Use

    Tags

    3xHA and Rluc8

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSEMS-Tom20-mEGFP-reHaloTagF

    Plasmid

    #200190

    Purpose

    Tom20 with reversible HaloTagF variant for fluorescent labeling

    Depositor

    Jacob Piehler

    Article

    Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

    Insert

    Tom20 (TOMM20 Human)
    

    Use

    Tags

    mEGFP and reHaloTagF

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSUMO-β′-COPI-WD40

    Plasmid

    #180753

    Purpose

    Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40 (residues 1-301).

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    β′-COPI-WD40 (SEC27 Budding Yeast)
    

    Use

    Tags

    N-terminal strep tag, His-tag, and SUMO tag

    Expression

    Bacterial

    Mutation

    Encodes residues 1-301; deleted 302-889; mutated …

    Promoter

    T7

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC-BAR-YR

    Plasmid

    #61766

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing bar gene (for resistance against glufosinate ammonium) on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Bar gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    trpC promoter

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEMS-Tom20-mEGFP-reHaloTagS

    Plasmid

    #200189

    Purpose

    Tom20 with reversible HaloTagS variant for fluorescent labeling

    Depositor

    Jacob Piehler

    Article

    Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

    Insert

    Tom20 (TOMM20 Human)
    

    Use

    Tags

    mEGFP and reHaloTagS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2582

    Plasmid

    #142436

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR1D1 (NR1D1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. kanMX6-ins6

    Plasmid

    #195043

    Purpose

    pFA6a derived selection cassette 5' flanked with tDEG1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

    Depositor

    Gloria Brar

    Article

    Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYpet-Optop38i

    Plasmid

    #89749

    Purpose

    Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i3
    

    Use

    Fluorescent protein

    Tags

    Ypet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. G31, Cdc20-AID tagging

    Plasmid

    #130269

    Purpose

    Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

    Depositor

    Matthias Heinemann

    Article

    Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

    Insert

    Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    

    Use

    Tags

    AID(71-114)

    Expression

    Yeast

    Mutation

    Promoter

    Native promoter

    Available since

    Sept. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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