We narrowed to 9,187 results for: Pol;

1. pBP-ORF

   Plasmid

   #72949

   Purpose

   Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 0 - ORFs
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCellFree_G03 CSNK2A1

   Plasmid

   #67085

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CSNK2A1 (CSNK2A1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pMpGWB106-OEP7(1–50)

   Plasmid

   #100602

   Purpose

   Binary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plants

   Depositor

   Yutaka Kodama

   Article

   Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

   Insert

   OEP7(1–50)-Citrine (OEP7 Mustard Weed)
   

   Expression

   Plant

   Available Since

   Feb. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p21 promoter F

   Plasmid

   #16467

   Depositor

   Bert Vogelstein

   Article

   el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

   Insert

   p21 promoter (CDKN1A Human)
   

   Expression

   Mammalian

   Available Since

   April 7, 2008

   Availability

   Academic Institutions and Nonprofits only

5. GAL 25Q+ProCFPp303

   Plasmid

   #15574

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt25Q + Pro (HTT Human)
   

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Proline-rich region present

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

6. pDEST15-Nedd4.2F823A

   Plasmid

   #165104

   Purpose

   bacterial expression of a GST fusion of Human Nedd4.2d F823A

   Depositor

   Arthur Haas

   Article

   Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

   Insert

   Nedd4.2d F823A (NEDD4L Human)
   

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   F823A; mutation for destabilization of trimer fo…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINK-TRABID (NZF1-3, aa 1-178)

   Plasmid

   #66185

   Purpose

   Expresses TRABID NZF1-3 (aa 1-178) in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   TRABID (ZRANB1 Human)
   

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   only codes for amino acids 1-178 of TRABID

   Promoter

   T7

   Available Since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

8. GAL HA25QDProCFPp303

   Plasmid

   #15578

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q Delta Pro (HTT Human)
   

   Tags

   CFP and HA

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. GAL HA103QDProCFPp303

   Plasmid

   #15579

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 103Q Delta Pro (HTT Human)
   

   Tags

   CFP and HA

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pET15b-GB

    Plasmid

    #129689

    Purpose

    express E. coli GreB

    Depositor

    Jeff Gelles

    Article

    Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    his6/thrombin/GreB (greB E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 CTNNB1

    Plasmid

    #67119

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CTNNB1 (CTNNB1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

    Plasmid

    #46839

    Purpose

    Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR_Hu_Plk5(WT)

    Plasmid

    #136329

    Purpose

    Human Plk5 WT in Gateway pENTR

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (PLK5 Human)
    

    Use

    Gateway entry vector

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. psicheck-2-Mrpl11-mod

    Plasmid

    #49569

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    Mrpl11-mod
    

    Use

    Luciferase

    Mutation

    T/A mutation in ATG start site

    Available Since

    Dec. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pET-NEMOL329P

    Plasmid

    #11969

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOL329P (IKBKG Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Leucine329 to Proline

    Available Since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pGBW-m4046831

    Plasmid

    #145671

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pKP4010

    Plasmid

    #113336

    Purpose

    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Inserts

    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    

    Tags

    3X-FLAG/8X-Gly linker

    Expression

    Yeast

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5 Halo RagB

    Plasmid

    #112753

    Purpose

    express Halo-RagB

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Halo-RagB (RRAGB Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLz74_Rubisco_CsoS2_Npeptide_fusion

    Plasmid

    #140840

    Purpose

    Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
    

    Tags

    CsoS2 N-peptide consensus to CbbL C-terminus and …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5A1-Ti

    Plasmid

    #92018

    Purpose

    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible catalytically inactive TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with 3 TEV cleavage sites
    Catalytically Inactive TEV Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V, C151A

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

    Plasmid

    #46838

    Purpose

    Express cGFP transcript ending in the minimal functional MALAT1 triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pIS1 IMP-1 short UTR

    Plasmid

    #21639

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pY71-tet.I.16

    Plasmid

    #133536

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. psicheck-2-Sfxn3-mod

    Plasmid

    #48170

    Purpose

    contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of SFXN3 (SFXN3 Human)
    

    Use

    Luciferase

    Mutation

    A/T mutation in the ATG of the uORF

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pCRII-TOPO CMV-cGFP-mMEN b_3' Sense

    Plasmid

    #46840

    Purpose

    Express cGFP transcript ending in the wildtype MEN beta triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCellFree_G03 GTF2H4

    Plasmid

    #67094

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H4 (GTF2H4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pIS1 IMP-1 rc

    Plasmid

    #21643

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCellFree_G03 MAPK13

    Plasmid

    #67077

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    MAPK13 (MAPK13 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pGST1-GGA1-VHS

    Plasmid

    #44418

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    GGA1-VHS (1-148) (GGA1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

30. FLAG-mPar6a WT

    Plasmid

    #86148

    Purpose

    FLAG tagged mouse Par6 wild type - Binds to aPKC, Par3, Lgl, Cdc42

    Depositor

    Senthil Muthuswamy

    Article

    Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

    Insert

    Par6 (Pard6a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    June 6, 2017

    Availability

    Academic Institutions and Nonprofits only