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Showing: 6841 - 6880 of 8371 results

  1. pLP-hSox2-ires-gfp

    Plasmid
    #49391
    Purpose
    mammalian bicistronic expression of human Sox2 and EGFP
    Depositor
    Randall McKinnon
    Insert
    Sox2 (SOX2 Human)
    Use
    Cre/Lox; Acceptor vector
    Tags
    6xHN, EGFP, and IRES
    Expression
    Mammalian
    Mutation
    TGA (stop) to CCA (Pro) at nt 1027 with respect t…
    Promoter
    CMV
    Available since
    Dec. 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  2. DNMT3B (3QKJ)

    Plasmid
    #32044
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DNMT3B (DNMT3B Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acids 293-442)
    Promoter
    Available since
    Feb. 22, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. AAV-SYN-PIK3CD

    Plasmid
    #203730
    Purpose
    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Insert
    PIK3CD (PIK3CD Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human synapsin promoter
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203730-plasmid-map-sequence-id-402632

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  5. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

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  6. pFP.R375

    Plasmid
    #138245
    Purpose
    Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138245-plasmid-map-sequence-id-273498

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  7. CD44I-c001

    Plasmid
    #175448
    Purpose
    Protein expression in bacterial cells (as inclusion bodies). CD44 Link module, M63-N172.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175448-plasmid-map-sequence-id-353140

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  8. pBABE.puro/TO/Flag/tGFP-HuR.wt

    Plasmid
    #110349
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tags
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    Promoter
    gag
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110349-plasmid-map-sequence-id-227289

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  9. pdCas9-sgRNA-com-BoxB-vector

    Plasmid
    #132556
    Purpose
    Expresses dCas9-DMNT3a-DMNT3l fusion protein, lambda N22-KRAB fusion protein, and sgRNA scaffold with com and BoxB replacing the Tetraloop and the loop 2 respectively.
    Depositor
    Baisong Lu
    Inserts
    dCas9-DMNT3a-DMNT3l (DNMT3A Synthetic)
    Lambda N22-KRAB
    sgRNA acaffold with com and BoxB replacing the Tetraloop and the loop 2.
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    132556-plasmid-map-sequence-id-261537

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  11. HDGF2

    Plasmid
    #32021
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HDGF2 (HDGFL2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    PWWP domain only (amino acid residues 1-93)
    Promoter
    T7
    Available since
    Jan. 3, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pFP.R271

    Plasmid
    #138220
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(IDE).SspDnaX.(CHT)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    138220-plasmid-map-sequence-id-296583

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  17. pSIREN-RetroQ-BPTF-sh28

    Plasmid
    #73669
    Purpose
    pSIREN-RetroQ vector containing shRNA sequence to BPTF
    Depositor
    Joe Landry
    Insert
    shRNA 28 to BPTF (BPTF, Bptf Human, Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 22, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    73669-plasmid-map-sequence-id-138080

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  19. pBABE.puro/TO/Flag/tGFP.HuR.S221D

    Plasmid
    #110351
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and turboGFP tags
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG and tGFP
    Expression
    Mammalian
    Mutation
    S221D, P237S
    Promoter
    gag
    Available since
    Nov. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    110351-plasmid-map-sequence-id-254627

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  21. pSIREN-RetroQ-BPTF-sh27

    Plasmid
    #73668
    Purpose
    pSIREN-RetroQ vector containing shRNA sequence to BPTF
    Depositor
    Joe Landry
    Insert
    shRNA #27 to BPTF (BPTF, Bptf Human, Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 13, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    73668-plasmid-map-sequence-id-141990

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  27. pFP.R365

    Plasmid
    #138235
    Purpose
    Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(HGK).EP-Pri*.(TEV)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    P(araBAD) and iPC
    Available since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138235-plasmid-map-sequence-id-273528

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  28. Act5C-pDONR221

    Plasmid
    #124242
    Purpose
    Gateway entry clone of Drosophila Actin5C cDNA.
    Depositor
    Peter Vilmos
    Insert
    Drosophila Actin 5C (Act5C Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124242-plasmid-map-sequence-id-240923

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  31. pTEI166

    Plasmid
    #126075
    Purpose
    Vector for expression of mitochondria targeted improved YFP1-10 (T65G, T205S, T203Y) in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Insert
    mitochondria Rieske iron sulphur protein (1-100 aa)
    Use
    Binary
    Tags
    YFP1-10 (improved)
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126075-plasmid-map-sequence-id-246135

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  32. pTEI128

    Plasmid
    #126209
    Purpose
    Module 1 vector of PlaMiNGo toolkit for cloning of candidate gene at N-terminus of GFP11x3 and expression in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    ccdB Cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary, module 1
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126209-plasmid-map-sequence-id-246907

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  33. 4RG2

    Plasmid
    #64658
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    53BP1 (TP53BP1 Human)
    Use
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pcDNA3.1 Flag YFP hNKCC1 L717C C723S C724V (NT531)

    Plasmid
    #50896
    Purpose
    Expresses human NKCC1 L717C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L717C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50896-plasmid-map-sequence-id-76369

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  36. Hs.KRAS4b G12D/C185S

    Plasmid
    #83165
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12D/C185S mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    G12D/C185S
    Promoter
    Available since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83165-plasmid-map-sequence-id-158128

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  37. 3JZN

    Plasmid
    #60123
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EED:APC056-C06:C32050 (EED Human, EED:APC056-C06:C32050)
    Use
    Tags
    6xHis and GST
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. GUCY1B3

    Plasmid
    #39121
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    GUCY1B3 (GUCY1B1 Human)
    Use
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    This is a complex of two proteins; GUCY1A3 (aa 46…
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  40. pGlomyc-Grhl1 (618 aa)

    Plasmid
    #236049
    Purpose
    Mammalian expression vector with myc-tagged mouse Grhl1 coding sequence (long isoform)
    Depositor
    Renee van Amerongen
    Insert
    Grhl1 (Grhl1 Mouse)
    Use
    Tags
    myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    236049-plasmid-map-sequence-id-474703

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Showing: 6841 - 6880 of 8371 results