We narrowed to 17,014 results for: Por

1. EGFP-Rab8a-wt

   Plasmid

   #86075

   Purpose

   A mammalian expression plasmid encoding Rab8a wild type with N-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTAMAHISTEV_PfeA

   Plasmid

   #128943

   Purpose

   expresses PfeA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

   Insert

   ferric enterobactin receptor
   

   Tags

   TEV protease cleavable 7xHis and TamA

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed (1-75)

   Promoter

   T7

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mRFP-Rab7

   Plasmid

   #14436

   Purpose

   Mammalian expression of Rab7 fused to mRFP. Used for localization to the late endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 7 (RAB7A Canis familialis)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   3 silent mutations near the C-terminus

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-Puro-CAG-GCaMP6s

   Plasmid

   #120896

   Purpose

   Donor plasmid targeting human AAVS1 safe harbor locus to generate GCaMP6s gene knockin. Contains two homologous arms flanking T2A-Puro resistance gene and CAG-driven GCaMP6s gene.

   Depositor

   Xiaojun Lian

   Article

   Jiang et al iScience. 2018 Oct 12;9:27-35. doi: 10.1

   Insert

   GCaMP6s
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPgiCas13b-gRNA-1

   Plasmid

   #184834

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPgiCas13b-gRNA-NT

   Plasmid

   #184835

   Purpose

   Constitutive expression of single-spacer CRISPR array with non-targeting spacer for PgiCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with a nontargeting spacer for PgiCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-C1-Ataxin3Q84

   Plasmid

   #22123

   Depositor

   Henry Paulson

   Article

   Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9_T2A_mCherry sgBFP

   Plasmid

   #192929

   Purpose

   All-in-one plasmid for cutting BFP region in DSB Spectrum V2

   Depositor

   Michael Yaffe

   Article

   van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

   Insert

   SpCas9
   

   Tags

   T2A mCherry

   Expression

   Mammalian

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_mScarlet-LAMP1

   Plasmid

   #185138

   Purpose

   Expresses mScarlet in mammalian lysosomes for pH quantification with fluorescence lifetime

   Depositor

   Andrew York

   Article

   Lazzari-Dean et al (2022), doi: 10.5281/zenodo.6363342

   Insert

   Lysosome-associated membrane glycoprotein 1 (Lamp1 Rat, Synthetic)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-21 of LAMP1

   Promoter

   CMV

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Puro CAG FUCCI

    Plasmid

    #136934

    Purpose

    donor plasmid for constitutive FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    

    Use

    Synthetic Biology

    Tags

    Clover and mKO2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N1 human cofilin S3E

    Plasmid

    #50861

    Purpose

    expresses psuedo-phosporylated, non-activatable cofilin fused to EGFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3E (CFL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S3E psuedo-phosporylated, non-activatable

    Promoter

    CMV

    Available Since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRE-UBC-HLA-E SCT-T2A-GFP

    Plasmid

    #205461

    Purpose

    lentiviral plasmid for expression of HLA-E SCT

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    HLA-E SCT (HLA-E Human)
    

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid

    #114110

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.

    Inserts

    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-Bcl-2-ΔBH4

    Plasmid

    #220300

    Purpose

    mammalian expression of Bcl-2-ΔBH4 tagged with GFP

    Depositor

    Xinlai Cheng

    Article

    Wang et al Cell Reports Physical Science, Volume 5,

    Insert

    Bcl2 (BCL2 Human)
    

    Tags

    Emerald GFP

    Expression

    Mammalian

    Mutation

    BH4 deleted

    Promoter

    CMV

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-SFPQ

    Plasmid

    #166959

    Purpose

    Expresses HA-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTight-hASCL1-N174

    Plasmid

    #31876

    Depositor

    Jerry Crabtree

    Article

    Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

    Insert

    achaete-scute complex homolog 1 (ASCL1 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1

    Plasmid

    #138418

    Purpose

    CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1

    Depositor

    Jian Xu

    Article

    Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    BioTAP-tag

    Promoter

    EF1a

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJAM1.19_PB-CIT-PGK-Blast

    Plasmid

    #205564

    Purpose

    Express human CIT

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    CIT (CIT Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-C1-Ataxin3Q28

    Plasmid

    #22122

    Depositor

    Henry Paulson

    Article

    Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLHCX-FLAG-mPKM2

    Plasmid

    #44239

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

    Insert

    Pyruvate Kinase M2 (Pkm Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

    Plasmid

    #194681

    Purpose

    CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_01-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. SIV_Rev

    Plasmid

    #236244

    Purpose

    3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Rev

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SIV Rev
    

    Use

    Lentiviral

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. MelanoTag-Control-PURO (pLJC5-GPR143-mScarlet-1xMyc)

    Plasmid

    #160368

    Purpose

    Stable expression of GPR143-mScarlet-1xMyc fusion protein for use as a control with MelanoTag-PURO

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    GPR143 (GPR143 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet-myc

    Available Since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. TRE-mClover3

    Plasmid

    #214922

    Purpose

    expression vector control - contitutive expression of mClover3 alone

    Depositor

    Ophir Shalem

    Article

    Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

    Insert

    mClover3
    

    Use

    Lentiviral

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTol2-ubi:loxP-mScarletNLS-SV40pA-loxp-QFGal4-SV40pA

    Plasmid

    #155125

    Purpose

    Tol2 vector containing Cre-inducible QFGal4-switch

    Depositor

    Bret Pearson

    Article

    Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

    Insert

    QFGal4-switch
    

    Use

    Cre/Lox; Zebrafish expression

    Promoter

    Ubi

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. SDHC-pHLuorin-sems

    Plasmid

    #107767

    Purpose

    labeling of mitochondrial succinate dehydrogenase subunit C, pH measurements

    Depositor

    Karin Busch

    Article

    Rieger et al Sci Rep. 2017 Apr 6;7:46055. doi: 10.103

    Insert

    Subunit C of SDH fused to pHluorin (SDHC Human)
    

    Tags

    pHluorin

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMKO.1 puro p53 shRNA 1

    Plasmid

    #10671

    Depositor

    William Hahn

    Article

    Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

    Insert

    p53 shRNA (TP53 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GFP-p38alpha

    Plasmid

    #86832

    Purpose

    mammalian expression of GFP-p38alpha

    Depositor

    Rony Seger

    Article

    Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

    Insert

    p38alpha (MAPK14 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-Miro1 S156A

    Plasmid

    #127588

    Purpose

    Phospho-mutant of S156

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Miro1 (RHOT1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S156A

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pConRef-2H12.D11

    Plasmid

    #221151

    Purpose

    cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen2)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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31. GFP-rab7 DN

    Plasmid

    #12660

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    T22N

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PMXS-SLC1A3

    Plasmid

    #72873

    Purpose

    The retroviral SLC1A3 vector was generated by cloning an human SLC1A3 PCR fragment (https://www.addgene.org/32813/)into the pMXS-ires-blast vector by Gibson Assembly

    Depositor

    David Sabatini

    Article

    Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

    Insert

    SLC1A3 (SLC1A3 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

33. MSCV-Luciferase-IRES-NrasG12D

    Plasmid

    #60834

    Purpose

    Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

    Depositor

    Scott Lowe

    Article

    Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

    Insert

    NrasG12D (Nras Mouse)
    

    Use

    Retroviral

    Available Since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET24a-VHH-APEX2

    Plasmid

    #109420

    Purpose

    Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to ascorbate peroxidase 2. VHH-APEX2 also contains a T7, HA, BAP and His6 epitope

    Depositor

    Martin Spiess

    Article

    Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

    Insert

    anti-GFP nanobody fused to a T7, APEX2, HA, BAP and His6 epitope
    

    Tags

    BAP, HA, His6, and T7

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SiC-V2-Cas9G7

    Plasmid

    #133043

    Purpose

    Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    Tet repressor
    

    Use

    Lentiviral

    Tags

    Cerulean

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid

    #49425

    Purpose

    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

    Depositor

    Daniel Klionsky

    Article

    Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

    Insert

    autophagy-related 8 (ATG8 Budding Yeast)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Promoter

    ATG8

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA-Flag-H3.3-polyA

    Plasmid

    #59780

    Purpose

    Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    H3F3B (H3-3B Human)
    

    Expression

    Mammalian

    Promoter

    CMV and T7

    Available Since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

    Plasmid

    #194683

    Purpose

    CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_05-mEGFP
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEasyG2_mic

    Plasmid

    #184916

    Purpose

    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

    Depositor

    Jeferson Gross

    Article

    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge