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We narrowed to 28,391 results for: REP

Showing: 5191 - 5220 of 28391 results

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pS6-TGFB2-H8

Plasmid

#128502

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t H8x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (L21-S414) (TGFB2 Human)

Use

Tags

His tag (6x)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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LV-hHOXA13

Plasmid

#135561

Purpose

Express hHOXA13 and EGFP in mammalian cells

Depositor

Jose Teixeira

Article

George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

Insert

Homeobox A13 (HOXA13 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CBH

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C1471S-FLAG

Plasmid

#198188

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C1471S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

The cysteine in STING at position 147 mutates to …

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

Plasmid

#176059

Purpose

Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pPN458

Plasmid

#137876

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRa;mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

Plasmid

#182425

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ-mNeongreen-hGH (GH1 Human)
VNp-LZ

Use

Tags

Vesicle Nucleating peptide (VNp) and Vesicle Nucl…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PEmax SpG

Plasmid

#187895

Purpose

All-in-one prime editor piggyBac transposon with PEmax, SpG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Phobos_CA

Plasmid

#98169

Purpose

slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue light, inactivation max with 580 nm (accelarates closure to ms). Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Phobos_C128A gene

Use

Tags

mCherry

Expression

Mammalian

Mutation

T59S, E83N, E90Q, E101S, V117R, E123S, C128A, T15…

Promoter

CMV (+enhancer)

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSBbi-RP-CoV2/stSpike-puro

Plasmid

#161794

Purpose

Transposon-based SARS-CoV-2 secretable trimeric Spike stable expression vector for protein production

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al Biotechnol Rep (Amst). 2023 Mar;37:e0078

Insert

SARS-CoV-2 secretable trimeric Spike

Use

Transposon

Tags

6xHis and dTomato

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G_FLAG-CRTC1/MAML2_PGK-GpNLuc

Plasmid

#154265

Purpose

Dox/Tet-inducible expression of the CRTC1-MAML2 fusion oncogene in mammalian cells.

Depositor

Antonio Amelio

Article

Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

Insert

FLAG-CRTC1/MAML2 fusion oncogene

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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nNOS/NOS1 scFv [L121/133]

Plasmid

#206759

Purpose

Mammalian Expression of nNOS/NOS1 scFV. Derived from hybridoma L121/133 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

nNOS/NOS1 (Homo sapiens) recombinant scFV (NOS1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PE xCas9

Plasmid

#172652

Purpose

All-in-one prime editor piggyBac transposon (xCas9)

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

xCas9_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GFP [N86/38R-1]

Plasmid

#206719

Purpose

Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/38-1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GFP (Aequorea victoria) recombinant Mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6

Plasmid

#170857

Purpose

AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.5 shETV4-B

Plasmid

#74976

Purpose

shETV4 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcmv3-STING-C64S,C65S-FLAG

Plasmid

#198186

Purpose

Mammalian expression of FLAG-tagged mouse STING1 C64S, C65S

Depositor

Yun Zhao

Article

Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

Insert

TMEM173 (Sting1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

The cysteine in STING at position 64/65 mutates t…

Promoter

CMV

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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VAPA/B scFv [N479/107]

Plasmid

#206742

Purpose

Mammalian Expression of VAPA/B scFV. Derived from hybridoma N479/107 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VAPA/B (Rattus norvegicus) recombinant scFV (Vapa Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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Anti-Rem2 [N324/2]

Plasmid

#190312

Purpose

Mammalian Expression Plasmid of anti-Rem2 (Rat). Derived from hybridoma N324/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Rem2 (Rattus norvegicus) recombinant Mouse monoclonal antibody (Rem2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

Plasmid

#115787

Purpose

Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

FZD4 (FZD4 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS3

Plasmid

#170854

Purpose

AAV backbone for Flp-dependent transgene expression of any transgene in neurons under the control of the hSyn1 promoter.

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Type

Empty backbone

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-HA-GFI1B

Plasmid

#125599

Purpose

doxycycline-inducible expression of human GFI1B (HA tagged) in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

Growth Factor Independent 1B transcriptional repressor (GFI1B Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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Synaptotagmin-7 scFv [N275/14]

Plasmid

#211608

Purpose

Mammalian Expression Plasmid of Synaptotagmin-7 scFv. Derived from hybridoma N275/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Synaptotagmin-7 (Rattus norvegicus) recombinant mouse scFv. (Syt7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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AAV8-hSyn-flex-miR30-eGFP-shCrh

Plasmid

#132715

Purpose

Encodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factor

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

GFP-shCrh (Crh Rat)

Use

AAV

Tags

Expression

Mutation

Promoter

Synapsin 1

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p-mCherry-C1-Aurora_CA

Plasmid

#98170

Purpose

slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.

Depositor

Peter Hegemann

Article

Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

Insert

Synthetic construct Aurora_C128A gene

Use

Tags

mCherry

Expression

Mammalian

Mutation

V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…

Promoter

CMV (+enhancer)

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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WRN scFv [N428/12]

Plasmid

#206784

Purpose

Mammalian Expression of WRN scFV. Derived from hybridoma N428/12 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

WRN (Homo sapiens) recombinant scFV (WRN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-GFP

Plasmid

#178713

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-dTom

Plasmid

#178715

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-STAT3

Plasmid

#195575

Purpose

Expresses STAT3

Depositor

Kevin Park

Article

Luo et al Cell Rep. 2016 Apr 12;15(2):398-410. doi

Insert

Signal transducer and activator of transcription 3 (Stat3 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV enhancer and promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Beta4-spectrin scFv [N393/2]

Plasmid

#190550

Purpose

Mammalian Expression of Beta4-spectrin scFV. Derived from hybridoma N393/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Beta4-spectrin (Homo sapiens) recombinant scFV (SPTBN4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC1 [1F1R]

Plasmid

#177437

Purpose

Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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