We narrowed to 5,640 results for: pcas

1. pT2K-CAGGS-T2TP

   Plasmid

   #114725

   Purpose

   expression in mammalian cells

   Depositor

   Martine Roussel

   Article

   Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

   Insert

   Tol2 transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. VIPR1-Tango

   Plasmid

   #66520

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   VIPR1 (VIPR1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pRubiC-T2A-Cas9

   Plasmid

   #75347

   Purpose

   Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.

   Depositor

   Bryan Luikart

   Article

   Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   mCherry (via t2a)

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPBT/EFS-Cas9-PuroTk

   Plasmid

   #170542

   Purpose

   Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   PuroTK
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GLuc-FLAG-CB1

   Plasmid

   #158066

   Purpose

   Membrane expression detection of Cannabinoid receptor 1

   Depositor

   Adolfo Rivero-Muller

   Article

   Rodriguez-Rodriguez et al Sci Rep. 2020 Oct 23;10(1):18191. doi: 1

   Insert

   CB1 (CNR1 Human)
   

   Use

   AAV

   Tags

   Gaussia Luciferase

   Expression

   Mammalian

   Mutation

   contains 4Kb of 3' UTR

   Promoter

   pCAG

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330-CANX

   Plasmid

   #227279

   Purpose

   Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of CANX (CANX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EF1a-hP53DD

   Plasmid

   #214080

   Purpose

   Mammalian expression of human P53DD

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   hP53DD (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-PITCh-TOMM20

   Plasmid

   #207789

   Purpose

   Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting C-terminus of TOMM20 (TOMM20 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

   Plasmid

   #160608

   Purpose

   TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   P35s:dCas9:SRDX:tNOS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-PITCh-CLTC

    Plasmid

    #227312

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CLTC (CLTC Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX459-TP53-exon4

    Plasmid

    #217455

    Purpose

    For TP53 knockout targeting exon 4 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt

    Plasmid

    #175563

    Purpose

    pSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1

    Depositor

    George Haughn

    Article

    Sola et al Plant Cell Physiol. 2021 May 27. pii: 62

    Insert

    GOXL1
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    RUBY

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX459-TP53-exon5

    Plasmid

    #217456

    Purpose

    For TP53 knockout targeting exon 5 of TP53

    Depositor

    John Diffley

    Article

    Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

    Insert

    CRISPR Cas9 guide for TP53 (TP53 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eTd-CBEm

    Plasmid

    #196602

    Purpose

    Mammalian expression of eTd-CBEm

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCPH2A.X_S139A-GS3

    Plasmid

    #204749

    Purpose

    Expression of Cas9 and human H2AX with the S139A mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139A (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the S139A mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. iCAP-BI-48-KanR

    Plasmid

    #203533

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI48 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. iCAP-BI-62-KanR

    Plasmid

    #203535

    Purpose

    RepCap for AAV production

    Depositor

    Ben Deverman

    Article

    Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

    Insert

    AAV-BI62 Cap
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330-PITCh-VIM

    Plasmid

    #227301

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of VIM (VIM Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. eTd-CBEa

    Plasmid

    #196603

    Purpose

    Mammalian expression of eTd-CBEa

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

    Insert

    bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS
    

    Use

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX330-RAB7A

    Plasmid

    #227297

    Purpose

    Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of RAB7A (RAB7A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SpyCas9 PE2*

    Plasmid

    #169850

    Purpose

    Mammalian Expression, SpyCas9 prime editor, optimized NLS

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    spCas9 PE*
    

    Use

    Tags

    bpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-PE2-tagRFP-BleoR

    Plasmid

    #192508

    Purpose

    Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.

    Depositor

    Gerald Schwank

    Article

    Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    PE2-P2A-tagRFP-P2A-BleoR
    

    Use

    Tags

    BleoR, SV40 bpNLS, and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3.1-RON-V5/HIS

    Plasmid

    #201983

    Purpose

    expression of human RON receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    RON receptor tyrosine kinase (MST1R Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pICSL11056

    Plasmid

    #68258

    Purpose

    Level 1 Golden Gate Cassette: Cas9 expression cassette for monocotyledonous plants

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Promoter/5UTR: ZmUbi + CDS:SpCas9 + 3UTR/terminator:35s
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCPH2A.X_S139D-GS3

    Plasmid

    #204748

    Purpose

    Expression of Cas9 and human H2AX with the S139D mutation

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al PLoS One. 2024 May 13;19(5):e0288578. do

    Insert

    H2AX S139D (H2AX Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    H2AX with the S139D mutation

    Promoter

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. SOX17-3'gRNA

    Plasmid

    #210465

    Purpose

    gRNA targeting SOX17 3' terminal for CRISPR-Cas9-mediated knock-in

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    SOX17 (SOX17 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. NKP38 GFP (1009)

    Plasmid

    #62021

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    AYTL1 (LPCAT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

29. DHFR-mNeon-GluA1

    Plasmid

    #173009

    Purpose

    For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Intracellular trafficking can be better visualized with mNeon.

    Depositor

    Matthew Kennedy

    Article

    Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

    Insert

    DHFR-mNeon-GluA1
    

    Use

    Tags

    DHFR and mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin (CAG)

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCb.CAG-HaloTag-GA-ZF-KrabA-KIF5C 378-955

    Plasmid

    #134613

    Purpose

    Mammalian expression of rat KIF5C 378-955

    Depositor

    Marvin Bentley

    Article

    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

    Insert

    KIF5C (Kif5c Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    WT with deletion of the 377 N-terminal residues.

    Promoter

    Chicken Beta actin

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge