We narrowed to 6,087 results for: nls

1. FRP2365

   Plasmid

   #127575

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Ura3 selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p3s-Cas9HC

   Plasmid

   #43945

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA epitope and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNXRVa-HACre

   Plasmid

   #78398

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   kanMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. FRP2371

   Plasmid

   #127577

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVITRO1-SS-247

   Plasmid

   #68761

   Purpose

   ZF9-TF-V2 with mCherry marker

   Depositor

   James Collins

   Article

   Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

   Inserts

   ZF9-TF-V2
   mCherry
   

   Use

   Tags

   NLS and flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. FRP2372

   Plasmid

   #127578

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-ACME

   Plasmid

   #186717

   Purpose

   ACME dual-deaminase CRISPR base editor

   Depositor

   Xue Gao

   Article

   Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

   Insert

   ACME
   

   Use

   CRISPR

   Tags

   Nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   See manuscript

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRP2374

   Plasmid

   #127579

   Purpose

   Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection marker

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
   

   Use

   Synthetic Biology; Shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS406-CMV-lacI-3FLAG

   Plasmid

   #83410

   Purpose

   Expression of lacI in yeast

   Depositor

   Toshi Tsukiyama

   Article

   Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

   Insert

   lacI repressor
   

   Use

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pISV-CRISPR/Cas9PGmUbi

    Plasmid

    #209189

    Purpose

    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection

    Depositor

    Christian Staehelin

    Inserts

    Cas9
    DsRed(deltaB)
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    missing BsaI site and plant-codon optimized with …

    Promoter

    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pME-24xMBS

    Plasmid

    #86246

    Purpose

    Middle entry vector for generating MBS tagged RNAs

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    NLS-tdMCP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMVJ-scTALEN-ABL1-R

    Plasmid

    #61695

    Purpose

    Expresses a single-chain TALEN targeting ABL1 site in human cells

    Depositor

    Huimin Zhao

    Article

    Sun et al Mol Biosyst. 2014 Mar 4;10(3):446-53. do

    Insert

    scTALEN-ABL1-R
    

    Use

    Tags

    Flag and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pIK86

    Plasmid

    #47933

    Purpose

    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.

    Depositor

    Iskra Katic

    Article

    Katic et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR and Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRVV655

    Plasmid

    #119019

    Purpose

    Drives TP901-1 recombinase expression in the D. melanogaster germ line

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2019 Jan 29. pii: g3.119.

    Insert

    TP901-1 recombinase
    

    Use

    Tags

    SV40 NLS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVITRO1-SS-113

    Plasmid

    #68737

    Purpose

    ZF9-TF with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF9-VP64
    mCherry
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pVITRO1-SS-148

    Plasmid

    #68749

    Purpose

    ZF8/ZF7-TF with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF8/ZF7-VP64
    mCherry
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVITRO1-SS-149

    Plasmid

    #68750

    Purpose

    ZF6/ZF10-TF with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF6/ZF10-VP64
    mCherry
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVITRO1-SS-127

    Plasmid

    #68733

    Purpose

    ZF5-TF with mCherry marker

    Depositor

    James Collins

    Article

    Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

    Inserts

    ZF5-VP64
    mCherry
    

    Use

    Tags

    NLS and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Gli3ZF in AINGpCAGGS

    Plasmid

    #121972

    Purpose

    Gli3 zinc fingers

    Depositor

    James Briscoe

    Insert

    Gli3ZF (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus and C terminus deletion only nt 1411-1…

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNXRVd-HACre

    Plasmid

    #78404

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    ura4+ (ura4 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge