We narrowed to 10,761 results for: T7
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Plasmid#110698PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertE. coli 16S toehold switch sensor
UseSynthetic BiologyPromoterT7Available SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21a - APX
Plasmid#89618PurposeExpresses recombinant 6HIS tagged T. cruzi ascorbate-dependent tryparedoxin peroxidase protein in E. coli upon IPTG inductionDepositorInsertAscorbate dependent Tryparedoxin peroxidase
Tags6HIS and T7 tag (gene 10 leader)ExpressionBacterialAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
TN-XXL pRSETB
Plasmid#45796PurposeFluorescent reporter for calcium signalingDepositorInsertTN-XXL
Tags6xHIS, ECFP, and YFPExpressionBacterialPromoterT7Available SinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-hDcp2 E147Q, E148Q
Plasmid#72215Purposebacterial expression of His-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorAvailable SinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgocatdead
Plasmid#210322PurposeBacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
Tags6x His tag and SUMO tagExpressionBacterialPromoterT7Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKAR1-NES
Plasmid#84632PurposeUnimolecular, FRET-based JNK activity reporter; cytosol targetedDepositorInsertECFP-FHA1-JNKsubDD-Citrine-NES
Tags6xHis, Citrine, ECFP, Nuclear export signal (NES)…ExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-HIS-ddFLN4-K10
Plasmid#101718PurposeK10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertddFLN4 fingerprint, K10 peptide (KRT10 Human, Dictyostelium discoideum)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHis-hPim1
Plasmid#100722PurposeExpression of human Pim1 kinase domain in E.coliDepositorInsertPim1 (PIM1 Human)
TagsHisExpressionBacterialMutationResidues 29-313 of isoform 2PromoterT7 promotorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR(T/A)
Plasmid#118473PurposeNegative-control mutant for blueCKAR biosensor.DepositorInsertblueCKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-TEV-GB1 Leu5Met
Plasmid#128686PurposeExpresses GB1 Leu5Met mutantDepositorInsertspg
TagsHisExpressionBacterialPromoterT7Available SinceApril 1, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pmCellFree_KA2_ZIKV NS4B
Plasmid#145367PurposeCell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherryDepositorInsertZIKV NS4B
Tags8xHis and mCherryExpressionBacterialPromoterT7- CMV/CAGAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS2B
Plasmid#145344PurposeCell-free/ mammalian expression vector for Zika virus NS2B protein with N-term eGFPDepositorInsertZIKV NS2B
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS4B
Plasmid#145347PurposeCell-free/ mammalian expression vector for Zika virus NS4B protein with N-term eGFPDepositorInsertZIKV NS4B
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-Syk-tSH2-PM
Plasmid#111271Purposeexpress murine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk tandem SH2 domains (Ser 8 to Gln 264), with Y130E mutation to mimic phosphorylation (Syk Mouse)
ExpressionBacterialMutationY130E mutation to mimic phosphorylationPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV PrM
Plasmid#145360PurposeCell-free/mammalian expression vector for Zika virus PrM protein with N-term mCherryDepositorInsertZIKV PrM
Tags8xHis and mCherryExpressionBacterialPromoterT7- CMV/CAGAvailable SinceMarch 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pExodus
Plasmid#39991DepositorTypeEmpty backboneExpressionBacterial and MammalianPromoterCMV, T7Available SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET14b_tev_PSGU
Plasmid#59738PurposeExpresses a.a. 417-887 of human ZO-1DepositorInsertzonula occludens-1 (TJP1 Human)
TagsHIS tag and thrombin clvgExpressionBacterialPromoterT7Available SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TmCA-P(11)4
Plasmid#185783PurposeEncodes thermostable carbonic anhydrase enzyme linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertTmCA-P(11)4
TagsHis-tagExpressionBacterialPromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIVT-EGFP-mouseCENP-C
Plasmid#174444Purposein vitro transcription template for mouse CENP-C (N-ter EGFP tagged)DepositorInsertCentromere protein C
UseIn vitro transcription templateTagsEGFPPromoterT7 SP6Available SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen1
Plasmid#78180PurposeExpresses rsGreen1 in E. coli strains.DepositorInsertrsGreen1
Tags6xHis-tag; Xpress tagExpressionBacterialPromoterT7Available SinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGST-GstZ1
Plasmid#163090PurposeExpressing mouse Gstz1 in bacterial cellsDepositorInsertsGstZ1
GstZ1
TagsGSTExpressionBacterialPromoterT7Available SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET42b-A3A-PBE-ΔUGI
Plasmid#119771PurposeTo express the recombinant APOBEC3A in vitroDepositorInsertAPOBEC3A-nCas9-NLS
Tags6*HIS and NLSExpressionBacterialMutationnCas9 has a mutation D10A in wild type SpCas9PromoterT7 promoterAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 STAT3
Plasmid#67120PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-CmCHS-CmCHI
Plasmid#73395PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
CmCHS
CmCHI
ExpressionBacterialPromoterT7Available SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
Affibody EGFR:1907.BirA*-pET301/NT-DEST
Plasmid#174691PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Pc4CL-CmCHS-MsCHI
Plasmid#73398PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsPc4CL
CmCHS
MsCHI
ExpressionBacterialPromoterT7Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cerulean-WW-NES
Plasmid#84629PurposeEncodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertCerulean-WW-NES
Tags6xHis, Cerulean, Nuclear export signal (NES), T7 …ExpressionMammalianPromoterCMVAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pet16b ALIX Bro1 (1-359)
Plasmid#80641PurposeResidues 1-359 of ALIX; Bacterial Expression Vector; N-term His-tag; Sundquist Lab Internal ID: WISP11-428DepositorInsertALIX Bro1, residues 1-359 (PDCD6IP Human)
TagsHis TagExpressionBacterialMutationResidues 1-359PromoterT7Available SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
B. fragilis 16S toehold switch sensor
Plasmid#110696PurposeToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP outputDepositorInsertB. fragilis 16S toehold switch sensor
UseSynthetic BiologyPromoterT7Available SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
TTR C10S S85C K80D
Plasmid#184743Purposebacterial expression of human TTR mutantsDepositorAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK_ToeRepG2_N02_trigger
Plasmid#132745PurposeT7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNADepositorInsertToeRepG2_N02_trigger
ExpressionBacterialAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL3
Plasmid#169108PurposeExpresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc - NanoLuc - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GATA1
Plasmid#67068PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT5
Plasmid#53560Purposebacterial expression of IFIT5DepositorAvailable SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_EPV-SBP-DsRed2-ER-5
Plasmid#170676PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.DepositorInsertsEPV-SBP-DsRed2-ER-5
streptavidin-KDEL
ExpressionMammalianPromoterIRES and T7Available SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_S-Luc_CLIP
Plasmid#113923PurposePlasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag for BRETDepositorInsertS-Luc_CLIP
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_ZIKV NS1
Plasmid#145342PurposeCell-free/mammalian expression vector for Zika virus NS1 protein with N-term eGFPDepositorInsertZIKV NS1
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable SinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-OSF-TRIM5-21R
Plasmid#79063PurposeGateway entry vector for cloning and propagation in DH5alphaDepositorInsertOSF-TRIM5-21R
TagsOneSTrEP-FLAGExpressionInsectPromoterT7Available SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[D134TAG+N150TAA]
Plasmid#164582Purposesuperfolder GFP with D134TAG and N150TAA mutationsDepositorInsertsfGFP-134TAG+150TAA
Tags6xHisExpressionBacterialMutationD134TAG + N150TAA MutationsPromoterT7Available SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS-Nvec-codonopti-Cas9-2xNLS
Plasmid#141108PurposePlasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensisDepositorInsertNematostella codon optimized Cas9
UseUnspecifiedMutationAll amino acids coded for by codons optimized for…PromoterT7Available SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only