We narrowed to 9,370 results for: bli

1. pUASp YFP Rac1 DN

   Plasmid

   #52234

   Purpose

   Drosophila expression of YFP-Rac1 dominant negative mutant

   Depositor

   Matthew Scott

   Insert

   Rac1 (Rac1 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   T17N; DN mutation

   Promoter

   GAL4

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pDEST1K18ms

   Plasmid

   #72646

   Purpose

   Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae

   Depositor

   Brian Kvitko

   Article

   Mijatovic et al Phytopathology. 2021 Dec;111(12):2176-21

   Type

   Empty backbone

   Use

   Sucrose counter-selection allelic exchange vector

   Available Since

   March 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNA3.1(-) Full length Her2(ERBB2)-Fc

   Plasmid

   #194330

   Purpose

   Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

   Depositor

   Alexander McLellan

   Insert

   Her2-Fc (ERBB2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA-GFP

   Plasmid

   #133009

   Purpose

   pFA carrying a sfGFP gene

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Insert

   sfGFP
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   tetR

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACP-ADRβ2 Control Plasmid

   Plasmid

   #101128

   Purpose

   Expression of ACP-Tagged β2 Adrenergic Receptor in Mammalian cells

   Depositor

   Ana Egana, New England Biolabs

   Insert

   Beta-2-Adrenergic Receptor (ADRB2 Human)
   

   Tags

   ACP-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

   Plasmid

   #85398

   Purpose

   humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein

   Depositor

   Thomas Oertner

   Inserts

   humanized photoactivated adenylyl cyclase
   red fluorescent protein
   

   Use

   AAV

   Tags

   cMyc Tag

   Expression

   Mammalian

   Mutation

   S27A

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HSD17B11

   Plasmid

   #39159

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HSD17B11 (HSD17B11 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   codon-optimized

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. Hs.KRAS4a G13D

   Plasmid

   #83170

   Purpose

   Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G13D mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G13D

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lamin A (R298C)-mRFP

   Plasmid

   #124269

   Purpose

   Lamin A mutant (R298C), C-terminally tagged with Mrfp. Mutation associated with CMT neuropathy.

   Depositor

   Eric Schirmer

   Insert

   LMNA (R298C) (LMNA Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   LMNA (R298C)

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSBtet-Bla/PAG1-TurboID-GAI

    Plasmid

    #198217

    Purpose

    Transposon vector (Addgene #60510) for doxycycline-inducible expression of a trimer with human PAG1, part of TurboID (after G257), and GAI (AAs 11-93) for gibberellic acid-inducible biotin labeling

    Depositor

    Eric Davis

    Insert

    PAG1 (PAG1 Human)
    

    Use

    Transposon

    Tags

    3’ part of TurboID, starting with G257 and amino …

    Expression

    Mammalian

    Mutation

    None

    Available Since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. huntingtin_V2095-V3138_CbioCflag

    Plasmid

    #194764

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV and P10

    Available Since

    July 27, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. huntingtin_T97-M2069_NbioCflag

    Plasmid

    #194763

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV and P10

    Available Since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. MAP3K3

    Plasmid

    #38937

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAP3K3 (MAPK3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pDONR221_SMAD2_HAe2_Bsd

    Plasmid

    #167166

    Purpose

    Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.

    Depositor

    Alexander Loewer

    Insert

    Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
    

    Use

    Repair template for gene editing in human cells

    Tags

    blasticidin

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. SDC229-B03

    Plasmid

    #58763

    Purpose

    bacterial expression of PDB 4PYZ

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4PYZ (USP7 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    Aug. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid

    #69548

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO-Tet-On-HDAC1-shRNA3

    Plasmid

    #90243

    Purpose

    Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)

    Depositor

    Roland Friedel

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Lentiviral

    Available Since

    May 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCS2+5'MT-wbp2nl

    Plasmid

    #172467

    Purpose

    expresses myc-tagged version of wild-type protein

    Depositor

    Sally Moody

    Article

    Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

    Insert

    wbp2nl
    

    Use

    Xenopus expression

    Tags

    Myc

    Promoter

    SP6

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ataxin3:1-431_Q79_BV

    Plasmid

    #209733

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    6xHis-SBP-TEV

    Expression

    Bacterial

    Promoter

    Polyhedrin

    Available Since

    Nov. 13, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. AKT3

    Plasmid

    #39038

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AKT3 (AKT3 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

21. MS2_SC2_S_Omicron

    Plasmid

    #180229

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Omicron (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Jan. 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. Syn-ChR-tdTomato

    Plasmid

    #99843

    Purpose

    AAV expression of ChR2(H134R)-tdTomato under the Syn promoter

    Depositor

    Gordon Shepherd

    Insert

    ChR2(H134R)-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    ChR2(H134R), carrying a single point mutation at …

    Promoter

    human synapsin I promoter

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MS2_SC2_S_WT

    Plasmid

    #175044

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    WT (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. human KANK1 Ankyrin Repeats pET151

    Plasmid

    #164053

    Purpose

    Expresses human KANK1 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

    Plasmid

    #39300

    Depositor

    Tim Mitchison, Jennifer Tirnauer

    Insert

    EB1 (MAPRE1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S16A, R17A, H18A, D19A, W23A and N25A

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

26. CYP7A1 (3SN5)

    Plasmid

    #32874

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP7A1 (CYP7A1 Human)
    

    Expression

    Bacterial

    Mutation

    amino acids 25-503 only

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

27. AAV-SYN-PIK3CD

    Plasmid

    #203730

    Purpose

    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Insert

    PIK3CD (PIK3CD Human)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Human synapsin promoter

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUASp YFP Rac1 CA

    Plasmid

    #52235

    Purpose

    Drosophila expression of YFP-Rac1 constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Rac1 (Rac1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

29. WDR5

    Plasmid

    #36900

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    mutation version of plasmid #60121 3UR4

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

30. Hs.KRAS4a Q61L

    Plasmid

    #83171

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61L

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPNT6-eGFP

    Plasmid

    #71303

    Purpose

    This vector can be used for homologous recombination and allows to obtain recombinant neomycin resistant fluorescent cells that can loose fluorescence after Cre expression

    Depositor

    Gian Luca Forni, Marco Musso

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LMNB2

    Plasmid

    #36896

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    LMNB2 (LMNB2 Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains Laminb2 Ig-like domain, amino acid resid…

    Available Since

    June 19, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

33. MAT2B

    Plasmid

    #39080

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAT2B (MAT2B Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

34. Hs.KRAS4b A146T

    Plasmid

    #83150

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    A146T

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pLPS-hAkt1-gfp

    Plasmid

    #49388

    Purpose

    mammalian expression of human Akt1 fused to EGFP

    Depositor

    Randall McKinnon

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Cre/Lox; Acceptor vector

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    tga to cca at nt 1725 with respect to GenBank ID …

    Promoter

    CMV

    Available Since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pCMV-T7-SpG-P2A-mScarlet (MNW006)

    Plasmid

    #174136

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Hs.KRAS4a Q61R

    Plasmid

    #83172

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. DHRS4

    Plasmid

    #39071

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    DHRS4 (DHRS4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pTXB1-CRP/CAP

    Plasmid

    #51563

    Purpose

    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    CRP/CAP
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. ASPH

    Plasmid

    #39094

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ASPH (ASPH Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only