We narrowed to 11,245 results for: ENA;

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pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Plasmid

#125229

Purpose

Expresses transmembrane intracellular NanoLuc in mammalian cells

Depositor

Alice Ting

Article

Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

Insert

IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pXW109Hg(RS-RinA)

Plasmid

#123150

Purpose

Mercury sensor with two-layered amplifier cascade. pSB4A3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30RinA-t-PrinA-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, PmerT, PhrpLE, PrinA

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSCIP-hTRAC

Plasmid

#154094

Purpose

Self-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chain

Depositor

Scott McComb

Article

Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

Insert

sgRNA and BAIT targeting human TRAC locus

Use

CRISPR

Expression

Mammalian

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-6

Plasmid

#163132

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1 Insulator ObLiGaRe/EPB71

Plasmid

#90018

Purpose

Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

Depositor

Eric-Paul Bennett

Article

Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

Insert

MCS flanked by insulator and inverted ZFN binding sites

Expression

Bacterial

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe-Neuroserpin

Plasmid

#58259

Purpose

Retroviral vector for expressing Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pGEX4T2-Snf1-cat

Plasmid

#52683

Purpose

expression of the Snf1 kinase domain in bacteria

Depositor

Benjamin Turk

Article

Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

Insert

SNF1 (SNF1 Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

encodes kinase domain only, aa 41-315

Promoter

tac

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pJMP1344

Plasmid

#119273

Purpose

knockdown folA in E. aerogenes

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA folA (E. aerogenes)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1274

Plasmid

#119266

Purpose

B. subtilis deltaTn7 site and flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

B. subtilis deltaTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1273

Plasmid

#119265

Purpose

E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

E. coli attTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1263

Plasmid

#119264

Purpose

B. subtilis attTn7 site and flanking sequence cloned into pACYC

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

B. subtilis attTn7

Expression

Bacterial

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP1106

Plasmid

#119249

Purpose

construction intermediate

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA NT1/RR1 (rfp)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf221

Plasmid

#12909

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf221

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pAtCas9-dead_2

Plasmid

#91166

Purpose

Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_D10A+H840A (dead)

Use

CRISPR

Expression

Plant

Mutation

D10A, H840A

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-ChRger1-TS-EYFP

Plasmid

#127244

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger1) for optogenetic activation with systemic delivery or for low-light activation. Driven by the CamKIIa promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger1-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

CamKIIa

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a-T2-NLuc

Plasmid

#135938

Purpose

Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

Depositor

Antonio Amelio

Article

Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

Insert

NanoLuciferase

Tags

T2 mitochondrial localization sequence

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pK RagA-RagC

Plasmid

#99649

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

10x His tag, Poly arginine linker, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMS1088

Plasmid

#80159

Purpose

pET-Duet for bacterial expression of Cys-NuG2(4x)-Ald and co-expression of Mycobacterium tuberculosis Formylglycine Generating Enzyme

Depositor

Marcelo Sousa

Article

Walder et al J Am Chem Soc. 2017 Jul 5. doi: 10.1021/

Insert

HisSUMO-Cys-4xNuG2-Ald

Tags

HisSUMO

Promoter

T7

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pPPC027.306

Plasmid

#171149

Purpose

Biopterin production pathway with J306 scRNA on pBBR1-GmR plasmid

Depositor

Jesse Zalatan

Article

Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

Inserts

J306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 and J3-BBa_J23117

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Kp-2

Plasmid

#163144

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

AOX1

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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