We narrowed to 18,427 results for: Met

1. FL tension sensor module

   Plasmid

   #101170

   Purpose

   Expresses the FL-based tension sensor module. The biosensor allows force measurements at 3-5 pN.

   Depositor

   Carsten Grashoff

   Article

   Ringer et al Nat Methods. 2017 Nov;14(11):1090-1096.

   Insert

   YPet(short)-FL-mCherry
   

   Use

   Retroviral

   Tags

   His-tag and cysteine

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-30

   Plasmid

   #172754

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-30
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaM-2

   Plasmid

   #172768

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-2
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ARH1-c003

   Plasmid

   #173093

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRH (ADPRH Human)
   

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E2-L357, codon optimized

   Available Since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. peCALWY-1

   Plasmid

   #22233

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-1 Zn(II) sensor (ATOX1 GFP's, Human)
   

   Expression

   Mammalian

   Mutation

   linker consists of 9 GGSGGS repeats

   Available Since

   Nov. 18, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pETM6-G6-vioABECD

   Plasmid

   #66536

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Mutant T7 Promoter - G6

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-CRY2(D387A)-mCherry

   Plasmid

   #58369

   Purpose

   Light insensitive CRY2 mutant

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Insert

   CRY2[1-498] (D387A) (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D387A

   Promoter

   CMV

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-1_VNp-Uricase

   Plasmid

   #182401

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-uricase fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-uricase
   

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-GFAP(long)-FLEX-GCaMP7f

   Plasmid

   #189612

   Purpose

   Cre-dependent GCaMP7f under the GFAP promoter

   Depositor

   Martin Riccomagno

   Article

   Agnew-Svoboda et al Cell Rep Methods. 2022 Aug 22;2(8):10027

   Insert

   GCaMP7f
   

   Use

   AAV and Cre/Lox

   Tags

   GCaMP7f

   Expression

   Mammalian

   Available Since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDuRCC-N

    Plasmid

    #81194

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please view depositor comments below and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU6_(GLuc)_INT[BoBS]

    Plasmid

    #68431

    Purpose

    Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRuby2-Zyxin-6

    Plasmid

    #55917

    Purpose

    Localization: Focal Adhesions, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Zyxin (ZYX Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBAD-TDsmURFP-RBS-HO-1

    Plasmid

    #80342

    Purpose

    Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

    Inserts

    Tandem Dimer smURFP
    HO-1
    

    Tags

    6 Histidine Tag

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Clover-PMP-N-10

    Plasmid

    #56305

    Purpose

    Localization: Peroxisomes, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    PMP (PXMP2 Human)
    

    Tags

    mClover2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pET21-pelB-LaG-41-4GS-LaG-2

    Plasmid

    #172766

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-41 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-41-4GS-LaG-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. MacroH2A.2-c002

    Plasmid

    #173089

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A2 (MACROH2A2 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    K167-K372

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB700 lenti gRNA Cerulean-zhang2.0 Weiss full

    Plasmid

    #79383

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    Zhang 2.0 + scaffold
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPL5618_pUG_MtxR

    Plasmid

    #60929

    Purpose

    PCR template vector for generating methotrexate resistance cassette.

    Depositor

    Robert Piper

    Article

    MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

    Insert

    Dihydrofolate reductase (DHFR Human)
    

    Use

    Loxp flanked deletion cassette template

    Mutation

    L22F F31S

    Promoter

    TEF1

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Yellow Cameleon-Nano50/pcDNA3

    Plasmid

    #51964

    Purpose

    mammalian expression of Yellow Cameleon-Nano50, an ultrasensitive Ca2+ indicator with Kd = 50 nM

    Depositor

    Takeharu Nagai

    Article

    Horikawa et al Nat Methods. 2010 Sep;7(9):729-32. doi:

    Insert

    Yellow Cameleon-Nano50
    

    Expression

    Mammalian

    Available Since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. MDO1-c014

    Plasmid

    #173103

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROD1 (MACROD1 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    S58-A325, codon optimized

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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