We narrowed to 18,889 results for: Tec

1. GGE038

   Plasmid

   #120785

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsDown_Zeta-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GGE067

   Plasmid

   #120784

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp_Zeta-NotI
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJY3948

   Plasmid

   #113338

   Purpose

   6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   2-pyrone synthase, codon optimized for Y. lipolytica
   

   Tags

   6XHis

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfB2226

   Plasmid

   #67533

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae X-4 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Expression

   Yeast

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR1055

   Plasmid

   #71823

   Purpose

   Express Streptococcus pyogenes nCas9 (H840A) nickase carrying two C-terminal SV40 NLS

   Depositor

   Jacob Corn

   Article

   Richardson et al Nat Biotechnol. 2016 Jan 20. doi: 10.103

   Insert

   T7pr_6xHis-MBP-TEV-SPynCas9 (H840A)-2xNLS
   

   Use

   CRISPR

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GB4068

   Plasmid

   #187810

   Purpose

   Module for the constitutive expression of Cup2:Gal4AD and copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).

   Depositor

   Diego Orzaez

   Article

   Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

   Insert

   copper-inducible dCasEV2.1
   

   Expression

   Plant

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pENTR2-R2-PBR-L4

   Plasmid

   #141017

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR2-L3-PBL-R1

   Plasmid

   #141016

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CLAPon

   Plasmid

   #213405

   Purpose

   Peroxidase (APEX) reporter for TEV protease cleavage

   Depositor

   Wenjing Wang

   Article

   Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

   Insert

   E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCfB2190

    Plasmid

    #67535

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCfB2227

    Plasmid

    #67536

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Expression

    Yeast

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR-P2rP4

    Plasmid

    #141015

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. tdEos-Talin-N-22

    Plasmid

    #57673

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Talin
    

    Tags

    tdEos

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pBT138b-proA

    Plasmid

    #138514

    Purpose

    P3g

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TAL2369

    Plasmid

    #36780

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    NCOR1-TALEN_Right (Ncor1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pCY 3090-07

    Plasmid

    #36232

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    Streptoalloteichus hindustanus bleomycin (Sh ble)
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-mCherry-Zeocin

    Available Since

    May 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB4786

    Plasmid

    #106142

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TALEN1259

    Plasmid

    #32284

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting gria3a (gria3a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Available Since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-1-p19-T7-EGFP100

    Plasmid

    #46309

    Depositor

    Judy Lieberman

    Article

    Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

    Inserts

    p19
    EGFP100 DNA-1
    linker
    EGFP100 DNA-2
    

    Use

    Pro-sirna

    Tags

    6XHis and GST

    Expression

    Bacterial

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pFREE2-xCas9-sgRNA

    Plasmid

    #179579

    Purpose

    Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

    Depositor

    Kevin Solomon

    Article

    Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

    Insert

    xCas9
    

    Expression

    Bacterial

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge