We narrowed to 5,300 results for: pcDNA3.1+

1. pcDNA3-optoB2-LYY-YFP

   Plasmid

   #75271

   Purpose

   G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   G-protein independent version of optoB2
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   point mutations at L72F,Y136G,Y224A

   Promoter

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-NLS-ZapCmR2

   Plasmid

   #59012

   Purpose

   Nuclear zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NLS-ZapCmR2 genetically encoded Zn(II) sensor
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pNLRP11major.MYC

   Plasmid

   #131196

   Purpose

   Mammalian expression of human NLRP11 188S

   Depositor

   Thomas Kufer

   Article

   Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

   Insert

   NLRP11 188S (NLRP11 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   188S

   Promoter

   pCMV

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. PH-dL5**

   Plasmid

   #96946

   Purpose

   PLC pleckstrin homology domain linked to FAP for CALI validation

   Depositor

   Marcel Bruchez

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   Pleckstrin homology domain
   

   Use

   Tags

   dL5 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-PH-dL5**

   Plasmid

   #96947

   Purpose

   Expresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validation

   Depositor

   Marcel Bruchez

   Article

   He et al Nat Methods. 2016 Mar;13(3):263-268. doi

   Insert

   Pleckstrin homology domain
   

   Use

   Tags

   EGFP and dL5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mc2 blast EF1a

   Plasmid

   #206223

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS2410-Tier1-PhCMV-Myr_mTagBFP2

   Plasmid

   #169535

   Purpose

   Tier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Prom1 SV8

   Plasmid

   #184011

   Purpose

   Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286).

   Depositor

   Peter Stoilov

   Article

   Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

   Insert

   Prom1 (Prom1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Wild type cDNA

   Promoter

   Available since

   May 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Septin 11 isoform III

   Plasmid

   #120319

   Purpose

   Septin 11 isoform III mammalian expression

   Depositor

   Angel de Blas

   Article

   Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

   Insert

   SEPT11 (Sept11 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-HA-ERK2 L232A

    Plasmid

    #8976

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L232A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA_DiCre_59.F2

    Plasmid

    #60207

    Purpose

    Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
    

    Use

    Tags

    FKBP12 and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 19-59 of the humanized version of Cre…

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Vh-HA-2xFRB

    Plasmid

    #20224

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    HA and Vh

    Expression

    Mammalian

    Mutation

    One copy of FRB has a T2098L mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pDN43

    Plasmid

    #61346

    Purpose

    mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Use

    Tags

    GS linker-AsLov2-biNLS11 and PKIt NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. mc2 mCherry

    Plasmid

    #206219

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-HA-ERK2 Y261A

    Plasmid

    #8977

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y261A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

16. mc2 neo

    Plasmid

    #206214

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CENP-B mCherry PP1 gamma

    Plasmid

    #45221

    Depositor

    Michael Lampson, Iain Cheeseman

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    CENP-B mCherry PP1 gamma
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVH72-Tier1-PhCMV-SEAP

    Plasmid

    #169560

    Purpose

    Tier-1 vector encoding PhCMV-driven SEAP expression containing a computationally optimized secretion peptide (PhCMV-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven human secreted placental alkaline phosphatase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cenp-B DBD INCENP mCherry

    Plasmid

    #45233

    Purpose

    Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B-DBD INCENP-Δcen mCherry
    

    Use

    Tags

    DNA binding domain of CENP-B and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. aq-CFP

    Plasmid

    #36073

    Depositor

    Narasimhan Gautam

    Article

    Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

    Insert

    G protein subunit alpha q (Gnaq Mouse)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    CFP inserted between amino acid 124 and 125

    Promoter

    CMV

    Available since

    Feb. 6, 2013

    Availability

    Academic Institutions and Nonprofits only