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We narrowed to 15,661 results for: genome

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Showing: 7001 - 7040 of 15661 results

  5. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  6. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available Since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  15. pHR-LS

    Plasmid
    #205438
    Purpose
    Expression of Gaussia luciferase (gLuc) and spectinomycin resistance (Spec) genes
    Depositor
    Masamichi Kamihira
    Article
    Huang et al J Biosci Bioeng. 2021 Nov;132(5):469-478
    Inserts
    Gaussia luciferase gene
    spectinomycin resistance gene
    Use
    Cre/Lox and Luciferase; Chlamydomonas expression
    Promoter
    Heat shock protein 70A (HSP70A)/ribulose bisphosp…
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205438-plasmid-map-sequence-id-405460

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  21. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid
    #199254
    Purpose
    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase
    Depositor
    Manuel Goncalves
    Article
    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117
    Insert
    High specificity S. pyogenes Cas9-D10A-KARA nickase
    Use
    CRISPR
    Tags
    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A K848A R1060A
    Promoter
    CAG promoter
    Available Since
    April 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199254-plasmid-map-sequence-id-391656

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Showing: 7001 - 7040 of 15661 results