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pET21-MRPP1_native+

Plasmid

#67865

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form)

Depositor

Walter Rossmanith

Article

Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

Insert

MRPP1 (TRMT10C Human)

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-2xFLAG-Atoh1ER-DsRed

Plasmid

#101735

Purpose

Expresses mouse Atoh1 fused to ER and DsRed

Depositor

Albert Edge

Article

Parker et al Hum Gene Ther Methods. 2014 Feb;25(1):1-

Insert

Atoh1 (Atoh1 Mouse)

Tags

2x FLAG, DsRed, and ER

Expression

Mammalian

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCLXSN-HA-CYLD

Plasmid

#27556

Depositor

Shao-Cong Sun

Article

Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

Insert

CYLD (CYLD Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

R156G and G179R

Available Since

Aug. 30, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA-HA-CYLD

Plasmid

#27547

Depositor

Shao-Cong Sun

Article

Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

Insert

CYLD (CYLD Human)

Tags

HA

Expression

Mammalian

Mutation

R156G and G179R

Available Since

Aug. 30, 2011

Availability

Academic Institutions and Nonprofits only
p6896 PHAGE-P CMVT N-HA-hE6AP II wt

Plasmid

#37603

Depositor

Peter Howley

Article

Martinez-Noel et al Mol Cell Biol. 2012 May 29.

Insert

E6AP II wt (UBE3A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV tet regulatable

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pJBL705

Plasmid

#140375

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-5BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-merlin 1-339

Plasmid

#168128

Purpose

Bacterial expression of human Nf2/merlin 1-339

Depositor

Tina Izard

Article

Primi et al Matrix Biol Plus. 2021 Jul 1;12:100074.

Insert

Merlin (NF2 Human)

Tags

Glutathione S-transferase (GST)

Expression

Bacterial

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSR-siKlf5

Plasmid

#41740

Depositor

Jonathan Katz

Article

Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

Insert

Klf5 (Klf5 Mouse)

Use

RNAi

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
pDESTFLAGHApuro3-Ago2K533AK570A

Plasmid

#14104

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala, Lys570 to Alanine

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

Plasmid

#191177

Purpose

Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (frameshifted)

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B

Plasmid

#120888

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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EK0387 SFFV-mTagBFP2-SGc(Bdnf.s1)cSG-mCherry (FLP-IN)

Plasmid

#191157

Purpose

Expression of a sensor for murine Bdnf 3' UTR in mammalian cells; mTagBFP2 marker, mCherry output; 90 bp long

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mTagBFP2:Bdnf.s1:mCherry

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 4

Plasmid

#36104

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 4 (Gng4 Mouse)

Tags

YFP

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pRS316-RGR-GFP-mm

Plasmid

#51059

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

Plasmid

#163610

Purpose

Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Tags

mTurquoise2

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 340-590

Plasmid

#11633

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal half of merlin isoform 2.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
MolBC

Plasmid

#92210

Purpose

Expression of H. influenzae MolBC (ABC Transporter) in E. coli

Depositor

Douglas Rees

Article

Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Ep

Inserts

MolC (HI1471 )
MolB (HI1470 )

Tags

8x Histidine Tag and Enterokinase Cleavage Site b…

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-HA

Plasmid

#120902

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA-iRPT

Plasmid

#140763

Purpose

Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE cassette

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

Inserts

iRFP713
PuroR
rtTAM2

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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