We narrowed to 43,164 results for: cha

1. pENTR-2B-Gata4

   Plasmid

   #98615

   Purpose

   Gateway entry vector for Gata4

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   Gata4 (Gata4 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLgw EcoDam-V5-SRF

   Plasmid

   #98602

   Purpose

   Mammalian DamID lentiviral vector forSRF with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   SRF (Srf Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8/GW/TOPO-ELK1

   Plasmid

   #98616

   Purpose

   Gateway entry TOPO TA cloning vector for ELK1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Q230R

   Promoter

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-2B-Nkx2-5 delta HD-G8

   Plasmid

   #98614

   Purpose

   Gateway entry vector NKX2-5 _HD

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   NKX2-5 _HD (Nkx2-5 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Deletion of the homeodomain. Replaced by 8 glycin…

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-GRP78-T453D

   Plasmid

   #235209

   Purpose

   Expresses GRP78 with T453D mutation in mammalian cells

   Depositor

   Amy Shiu Lee

   Article

   Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

   Insert

   78-kDa glucose-regulated protein (HSPA5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Change Threonine 453 to Aspartic Acid

   Promoter

   Available since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-GRP78-R197H

   Plasmid

   #235208

   Purpose

   Expresses GRP78 with R197H mutation in mammalian cells

   Depositor

   Amy Shiu Lee

   Article

   Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

   Insert

   78-kDa glucose-regulated protein (HSPA5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Change Arginine 197 to Histidine

   Promoter

   Available since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-GRP78-G227D

   Plasmid

   #235207

   Purpose

   Expresses GRP78 with G227D mutation in mammalian cells

   Depositor

   Amy Shiu Lee

   Article

   Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

   Insert

   78-kDa glucose-regulated protein (HSPA5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Change Glycine 227 to Aspartic Acid

   Promoter

   Available since

   June 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-SFFV-RTN3-WPRE-UbC-Emerald

   Plasmid

   #233467

   Purpose

   Lentiviral vector plasmid expressing human reticulon 3 (RTN3) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva, Evan Reid

   Inserts

   RTN3 (RTN3 Human)
   Emerald
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV and UbC

   Available since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-3xFlag-SPNS1

   Plasmid

   #198568

   Purpose

   SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1 with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2C-EGFP-t2a-mCD4

    Plasmid

    #216213

    Purpose

    Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

    Depositor

    Michelle Percharde

    Article

    Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

    Insert

    CD4 (Cd4 Mouse)
    

    Use

    Tags

    GFP-T2A

    Expression

    Mammalian

    Mutation

    CD4 transmembrane domain only

    Promoter

    MERVL

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4 TAPBPR-TM-TN6

    Plasmid

    #178650

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    E205K, R207E, Q209S, Q272S, switched transmembran…

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-2B-Nkx2-5YRD Y-A

    Plasmid

    #98611

    Purpose

    Gateway entry vector for NKX2-5 YRD Y-A

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 YRD Y-A (Nkx2-5 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Substitutions of 7 tyr into Ala residues.Y233A; Y…

    Promoter

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid

    #110824

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-ASAP3-WPRE

    Plasmid

    #132331

    Purpose

    expresses ASAP3(ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

    Depositor

    Michael Lin

    Article

    Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

    Insert

    ASAP3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG414GPD-dCas9-VPR

    Plasmid

    #63801

    Purpose

    pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    April 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Integrin Beta1-mPA-GFP

    Plasmid

    #186302

    Purpose

    Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

    Depositor

    Pakorn Kanchanawong

    Article

    Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

    Insert

    Integrin Beta 1 (ITGB1 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 SIRT1-FL

    Plasmid

    #105670

    Purpose

    Mammalian expression construct of mouse SIRT1 full length isoform

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cav2.1 HA pcDNA3

    Plasmid

    #206069

    Purpose

    expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPICZc-ScAct1*-thymosinB-8His

    Plasmid

    #111148

    Purpose

    Expresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    Act1 (ACT1 Budding Yeast)
    

    Use

    Pichia pastoris integration plasmid

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMXs-IRES-blast GFP-SPNS1

    Plasmid

    #198571

    Purpose

    SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

    Depositor

    Monther Abu-Remaileh

    Article

    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

    Insert

    SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
    

    Use

    Retroviral

    Tags

    3xFlag-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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