We narrowed to 7,778 results for: CCH
-
Plasmid#232882PurposePlasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only
-
pML107-YFR054C
Plasmid#232900PurposePlasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-ZIM17
Plasmid#232901PurposePlasmid expressing Cas9 and gRNA AAATGTCTCACTTTGCAGTG which targets the ZIM17 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-SRP14
Plasmid#232896PurposePlasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-TLG1
Plasmid#232897PurposePlasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-VHT1
Plasmid#232898PurposePlasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-HIS3-2
Plasmid#232881PurposePlasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-ADE13
Plasmid#232887PurposePlasmid expressing Cas9 and gRNA GTAGCAGCAAAAGAAGACAA which targets the ADE13 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-LAS17
Plasmid#232892PurposePlasmid expressing Cas9 and gRNA AATACCCTGAATTCTGCCGG which targets the LAS17 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-LEU2-2
Plasmid#232883PurposePlasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS313-rad53-5004
Plasmid#232902PurposePlasmid carrying the rad53-5004 allele with its native promoter and terminator.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-INO80
Plasmid#232890PurposePlasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-Htt(25Q)-c_myc
Plasmid#225228PurposeYeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.DepositorInsertsAga2
Htt (25Q)
TagsHA Tag and c-myc TagExpressionYeastAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-Htt(46Q)-c_myc
Plasmid#225229PurposeYeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.DepositorInsertsAga2
Htt (46Q)
TagsHA Tag and c-myc TagExpressionYeastAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAM90
Plasmid#226682PurposeRpn9S111TAG lid (Rpn5, MBP-HRV-Rpn6, Rpn8, Rpn9S111TAG, Rpn11)DepositorInsertsTagsMBP-HRVExpressionBacterialMutationS111TAGAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-D3-ASK1
Plasmid#226619PurposeCo-expression of D3 and ASK1 complex in insect cellsDepositorInsertsTagsHis tag followed with three copies of MsybExpressionInsectMutationThe flexible loop in D3 (Q478-D515) can be remove…PromoterPolyhedrinAvailable SinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxamEXP4CUP1
Plasmid#218284PurposeUse together with pJE13HD7 and pJE13HD7 derivatives to integrate RelE expression cassette under the control of the CUP1 promoter at ho locusDepositorInsertHph>tAgTEF1-ISceI-Repeat2-pCUP1>RelE(1-125)>RPL28intron>RelE(126, 289)>tTPI1-HO(1828, 1979)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL43C
Plasmid#218278PurposeIntroduction of a LowTempGAL Turbo module (GAL43C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL4A
Plasmid#218277PurposeIntroduction of a LowTempGAL Turbo module (GAL4) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-fcy1(454-778)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRGAL3C
Plasmid#218275PurposeIntroduction of a LowTempGAL Turbo module (GAL3C) to accelerate induction of GAL promoter inductionDepositorInsertfcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2>GAL3C(S509P)>tENO2-fcy1(454-778)
ExpressionYeastMutationGAL3 (S509P)Available SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K48R)-GFP
Plasmid#212793PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeastDepositorInsertHis6-CUbo(K48R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K48R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-His6-CUbo(K63R)-GFP
Plasmid#212794PurposeCopper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeastDepositorInsertHis6-CUbo(K63R)-GFP
UseIntegrative vectorExpressionYeastMutationCUb(K63R)PromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-NUbo-E3(48) aa24-150-VSV
Plasmid#212747PurposeConstitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)DepositorInsertE3(48) aa24-150
UseIntegrative vectorTagsNUbo and VSVExpressionYeastMutationCue1 ΔU7BDPromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp211-ADH-myc-E3(1) C885A-CUbo-VSV
Plasmid#212738PurposeConsitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)DepositorInsertE3(1)
UseIntegrative vectorTagsCUbo-NLS-VSV and mycExpressionYeastMutationHOIP(C885A)PromoterADH1Available SinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-GW-dCas9-SET2-R195G-C201A-IRES2-mCherry
Plasmid#202090PurposeNeuron-specific expression vector of catalytically-dead dCas9-Set2 containing R195G and C201A mutationsDepositorInsertdCas9-Set2(R195G, C201A)
Tags3x FLAG and mCherryExpressionMammalianMutationR195G and C201A abolish catalytic activityPromoterhuman SynapsinAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
Plasmid#212805PurposeConstitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cellsDepositorInsertNUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7
ExpressionMammalianAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN HA-ATG13(Δ1-198)
Plasmid#186222PurposeFor expression of ATG13(Δ1-198) tagged with HADepositorInsertATG13 (ATG13 Human)
UseRetroviralTagsHAExpressionMammalianMutationDeleted amino acids 1 - 198Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K
Plasmid#198037PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)DepositorAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastAvailable SinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2
Plasmid#185346PurposeMembrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2DepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
ExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG
Plasmid#185347PurposeMembrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tagDepositorInsertCue1p/Ube2G2 fusion (internal MYC tag)
TagsFLAGExpressionMammalianAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT
Plasmid#184774PurposeMarker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGS_591
Plasmid#160653PurposeExpresses OLE1 under the control of the yeast Sup35 promoterDepositorAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (M)
Plasmid#182434PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (G)
Plasmid#182476PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL1 and GAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIALD2HMGr
Plasmid#185867PurposeKnocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiaeDepositorInsertALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
ExpressionYeastMutationNoneAvailable SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPOLY_new
Plasmid#179276PurposeExpresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)DepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBICI_high ori
Plasmid#179273PurposeExpresses CuCBP and BaSP in E. coli BL21(DE3)DepositorInsertsSucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda
UseSynthetic BiologyTagsHis Tag and Strep - Tag IIExpressionBacterialPromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDUBI
Plasmid#179274PurposeExpresses CcCDP monocistronically and CuCBP and BaSP bicistronicallyDepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
C8077_RS00435 sucrose phosphorylase [ Bifidobacterium adolescentis (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialMutationsucrose phosphorylasePromoterT7 lacOAvailable SinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only