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1375_pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-CB-SACas9-HA-OLLAS-spA

Plasmid

#109311

Purpose

Plasmid for AAV SaCas9 Mammalian Expression with a gRNA against Ldlr

Depositor

William Lagor

Article

Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

Insert

Ldlr gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pKnockinDonor-humanCTCF-miniAID-mClover3

Plasmid

#136883

Purpose

knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

Depositor

Chunliang Li

Article

Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

Insert

CTCF-knockin-homology arm-miniAID-mClover3

Tags

miniAID-mClover3

Expression

Mammalian

Promoter

no promoter

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-KIF20A

Plasmid

#192297

Purpose

Gateway entry vector for an inducible 3xFLAG-KIF20A

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

KIF20A (KIF20A Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

Plasmid

#187114

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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K15/LacZ

Plasmid

#44265

Depositor

George Cotsarelis

Article

Liu et al J Invest Dermatol. 2003 Nov;121(5):963-8

Insert

K15 promoter (-4.8) (Krt15 Mouse)

Use

Mouse Targeting; Β-galactosidase

Tags

LacZ

Expression

Mammalian

Mutation

Contains the murine K15 promoter (-4.8) fragment

Promoter

murine K15 promoter (-4.8) fragment

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-MED8

Plasmid

#15406

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED8 (MED8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEW4: pET-32a Dot1L(2-386)

Plasmid

#124099

Purpose

Dot1L(2-386) expression plasmid

Depositor

Cynthia Wolberger

Article

Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

Insert

Dot1L(2-386) (DOT1L Human)

Tags

Trx-His-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-D2s-L-V2

Plasmid

#19968

Depositor

Jonathan Javitch

Article

Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

Insert

D2 short variant-C terminal Venus (DRD2 Human, Aequorea Victoria)

Tags

C terminal Venus and Flag

Expression

Mammalian

Available Since

Jan. 27, 2009

Availability

Academic Institutions and Nonprofits only
Human HMG-CoA lyase like 1 (HMGCLL1)

Plasmid

#52434

Purpose

mammalian expression of human HMG-CoA lyase like 1 (HMGCLL1)

Depositor

Henry Miziorko

Article

Montgomery et al J Biol Chem. 2012 Sep 28;287(40):33227-3

Insert

HMGCLL1 (HMGCLL1 Human)

Tags

HIS

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2014

Availability

Academic Institutions and Nonprofits only
pDONR223 LC3A G120A

Plasmid

#123070

Purpose

Gateway entry clone encoding human MAP1LC3A G120A

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Use

Gateway entry vector / entry clone

Mutation

Glycine 120 to Alanine

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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MAC_N_ELK3

Plasmid

#167750

Purpose

Expresses ELK3 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELK3 (ELK3 Human)

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-N1-Lyn11

Plasmid

#202683

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

LYN(1-11):PAmCherry1 (LYN Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAcrF2

Plasmid

#89234

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

AcrF2

Tags

6xHis-TEV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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RCAS(A) dRhoA V14 (CT#846)

Plasmid

#13949

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

RhoA (Rho1 Fly)

Use

Retroviral; Avian expression

Mutation

G14V mutation

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
Lyn11C3S-EGFP

Plasmid

#202747

Purpose

weakly plasma membrane targeted fluorescent protein

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

LYN (1-11) (C3S):EGFP (LYN Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB4 (E317K)

Plasmid

#29528

Depositor

Yardena Samuels

Article

Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

Insert

ERBB4 (ERBB4 Human)

Expression

Mammalian

Mutation

changed glutamic acid 317 to lysine

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
pGEX4T-2 MED8

Plasmid

#15436

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED8 (MED8 Human)

Tags

GST

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) dRhoA N19 (CT#847)

Plasmid

#14002

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

RhoA (Rho1 Fly)

Use

Retroviral; Avian expression

Mutation

T19N

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Lin28B-mCCHC

Plasmid

#51375

Depositor

Narry Kim

Article

Heo et al Cell. 2009 Aug 21;138(4):696-708. doi: 1

Insert

Lin28B (LIN28B Human)

Tags

FLAG

Expression

Mammalian

Mutation

CCHC domain mutant (H137A, H159A)

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pT7-V5-SBP-C1-Hs4EBP3_Z

Plasmid

#148151

Purpose

Mammalian Expression of Hs4EBP3

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

Hs4EBP3 (EIF4EBP3 Human)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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