We narrowed to 4,337 results for: U6 gRNA

1. lentiCRISPR v2-sgDHODH-2

   Plasmid

   #186023

   Purpose

   knock out DHODH in mammalian cells

   Depositor

   Boyi Gan

   Article

   Mao et al Nature. 2021 May;593(7860):586-590. doi:

   Insert

   Dihydroorotate Dehydrogenase (Quinone) (DHODH Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AllCB shRNA-1

   Plasmid

   #160065

   Purpose

   Knock Down all Collybistin isoforms

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   Promoter

   mouse U6 promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiCRISPRv1_SDHA

   Plasmid

   #177980

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHA (SDHA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMKO.1-shCcnb2

   Plasmid

   #162794

   Purpose

   Ccnb2 shRNA in pMKO.1 retroviral vector

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Ccnb2 shRNA (Ccnb2 Mouse)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAX198

   Plasmid

   #173042

   Purpose

   Custom vector for sgRNA library construction

   Depositor

   Brittany Adamson

   Article

   Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

   Insert

   HBB Gene - Second and third exons of ENST00000647020.1 (no intron) and part of the 3' UTR (HBB Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PLL5.0-Anillin ShRNA-Cherry-AnillinFL

   Plasmid

   #187271

   Purpose

   Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin full length

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   U6, CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLL5.0-Anillin shRNA

   Plasmid

   #187270

   Purpose

   Lentiviral expression of shRNA targeting Anillin

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   shRNA for Anillin (ANLN Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   U6

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pX330-mitf

   Plasmid

   #69801

   Purpose

   Used to make mitf knockout porcine cell line

   Depositor

   Jianguo Zhao

   Article

   Wang et al Sci Rep. 2015 Aug 21;5:13348. doi: 10.10

   Inserts

   mitf-sgRNA
   SpCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta-Actin and U6

   Available since

   Dec. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLKO-RFP-IKZF1-sh2

   Plasmid

   #69041

   Purpose

   3rd generation lentiviral vector expressing IKZF1 shRNA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF1 (IKZF1 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiCRISPR-v1-sgGLS_2

    Plasmid

    #163459

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting GLS

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 2 targeting GLS (GLS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJZC33

    Plasmid

    #62330

    Purpose

    sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2 binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO-RFP-IKZF1-sh3

    Plasmid

    #69042

    Purpose

    Lentiviral expression of IKZF1 shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pY036_ATP1A1_G5_Array

    Plasmid

    #86620

    Purpose

    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via hdr using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO-shSmad2

    Plasmid

    #37051

    Depositor

    Sam Thiagalingam

    Article

    Papageorgis et al Cancer Res. 2010 Feb 1;70(3):968-78. Epu

    Insert

    Smad2 (SMAD2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

15. iMAP-61

    Plasmid

    #187460

    Purpose

    gRNA array of iMAP-61

    Depositor

    Tian Chi

    Article

    Liu et al Cell. 2022 Aug 4;185(16):3008-3024.e16.

    Insert

    sgRNA array
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified U6

    Available since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJZC48

    Plasmid

    #62336

    Purpose

    sgRNA + 1x COM with COM-VP64 effector for mammalian cells

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x COM binding module
    COM-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCRISPR-v1-sgGLS_6

    Plasmid

    #163460

    Purpose

    lentiviral vector expressing Cas9 and an sgRNA targeting GLS

    Depositor

    Jason Cantor

    Article

    Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

    Insert

    sgRNA 6 targeting GLS (GLS Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pY036_ATP1A1_G3_Array

    Plasmid

    #86619

    Purpose

    Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO-RFP-IKZF3-sh3

    Plasmid

    #69044

    Purpose

    3rd generation lentiviral vector expressing IKZF3 shRNA

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF3 (IKZF3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLL5.0 hCortKD-Flcortmut3YD EGFP

    Plasmid

    #187264

    Purpose

    Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

    Depositor

    Alpha Yap

    Article

    Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

    Insert

    Cortactin (CTTN Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    3YD (Y412, Y470, Y486)

    Promoter

    U6, 5'LTR

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge