164,802 results

1. ATG9A-mCherry

   Plasmid

   #197393

   Purpose

   Expresses ATG9A in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

   Insert

   ATG9A (ATG9A Human)
   

   Use

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUCmini-iCAP-PHP.B

   Plasmid

   #103002

   Purpose

   non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

   Depositor

   Viviana Gradinaru

   Article

   Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

   Insert

   Synthetic construct isolate AAV-PHP.B VP1 gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   p41

   Available Since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-Padron2

   Plasmid

   #172355

   Purpose

   Expresses the reversibly switchable fluorescent protein Padron2 in bacterial cells

   Depositor

   Stefan Jakobs

   Article

   Konen et al ACS Nano. 2021 May 21. doi: 10.1021/acsn

   Insert

   Padron2
   

   Use

   Tags

   6xHisTag (N terminal on backbone)

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-NLS-dPspCas13b-3xmRuby3-NLS

   Plasmid

   #132405

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPspCas13b
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H133A; H1059A

   Promoter

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiGuide-mCherry

   Plasmid

   #217005

   Purpose

   lentiviral plasmid for gRNA cloning with selectable mCherry expression

   Depositor

   Deepta Bhattacharya

   Article

   D'Souza et al PLoS One. 2022 Aug 24;17(8):e0261801. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-HisD-miniGFP1

   Plasmid

   #188896

   Purpose

   Express 6xHis-Tag miniGFP1 in E.coli

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. prDNA spacer-LacOx120-20xMS2-Hyg

   Plasmid

   #210220

   Purpose

   Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Inserts

   120xLacO repeats derived from: PMID: 12864855.
   24xMS2 repeats derived from Addgene plasmid # 31865
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKB225

   Plasmid

   #170015

   Purpose

   Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter

   Depositor

   Jeffrey Tabor

   Article

   Brink et al bioRxiv

   Inserts

   mNeonGreen
   Peptide display protein with no C-terminal peptide
   

   Use

   Synthetic Biology

   Tags

   Lpp, OmpA, and Tether

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac and PvirK

   Available Since

   Nov. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. FH-Tet2-pEF

   Plasmid

   #41710

   Purpose

   This plasmid expresses mouse Tet2 with N-terminal FLAG and HA tags in a mammalian system

   Depositor

   Anjana Rao

   Article

   Ko et al Nature. 2010 Dec 9;468(7325):839-43. doi

   Insert

   tet methylcytosine dioxygenase 2 (Tet2 Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

    Plasmid

    #207507

    Purpose

    This plasmid can be used to generate retrovirus.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, HA-GD2-28z_CAR (NGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. eSpCas9(1.1)_No_FLAG_AAVS1_T2

    Plasmid

    #79888

    Purpose

    Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO-SOX9

    Plasmid

    #170703

    Purpose

    doxycycline-inducible overexpression of SOX9

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SOX9 (SOX9 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

    Plasmid

    #221235

    Purpose

    Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-TO-dCas9-3XGFP

    Plasmid

    #64107

    Purpose

    Expression of Sp dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPET-PKR/PPase

    Plasmid

    #42934

    Depositor

    James Cole

    Article

    Lemaire et al J Mol Biol. 2005 Jan 7;345(1):81-90.

    Inserts

    protein kinase R (EIF2AK2 Human)
    phage lambda phosphatase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbA6c-RFP

    Plasmid

    #35290

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-SF3B1-K700E-reporter

    Plasmid

    #200318

    Purpose

    A fluorescent reporter for SF3B1 K700E mutation. Co-expresses a blasticidin resistance gene for selection. Includes LoxP sites for Cre-mediated deletion of reporter cassette.

    Depositor

    Lili Wang

    Article

    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.

    Insert

    BSD-2A-EGFPi
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. shYAP1 # 2

    Plasmid

    #42541

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-PGK-Neo-PIP-FUCCI

    Plasmid

    #118616

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-N1-MITF-A

    Plasmid

    #38132

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    MITF-A (MITF Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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