We narrowed to 10,521 results for: SEC

Showing: 7081 - 7120 of 10521 results

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pUASp YFP RabX5 CA

Plasmid

#37779

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

RabX5 (RabX5 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q123L (constitutive active)

Promoter

GAL4

Available Since

Nov. 19, 2012

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab2 WT

Plasmid

#37684

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab2 (Rab2 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

Nov. 16, 2012

Availability

Academic Institutions and Nonprofits only
UAS-zw4 (egghead) ORF

Plasmid

#38764

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

zw4 (egh Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pCaShs-egghead (zw4)

Plasmid

#37400

Depositor

Norbert Perrimon

Article

Goode et al Development. 1996 Dec;122(12):3863-79.

Insert

egghead (egh Fly)

Expression

Insect

Promoter

Hsp70

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
GFP-Rab19

Plasmid

#31741

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab19 (Rab19 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pMT-Pygopus

Plasmid

#31900

Depositor

Robert Tjian

Article

Wright et al Proc Natl Acad Sci U S A. 2009 Jan 6;106

Insert

Pygopus (pygo Fly)

Tags

6xHIS and V5

Expression

Insect

Promoter

P-MT (Metallothionein)

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcasper4 p [7-Gr66a-]

Plasmid

#26860

Depositor

Craig Montell

Article

Moon et al Curr Biol. 2006 Sep 19. 16(18):1812-7.

Insert

Gr66 gustatory receptor (Gr66a Fly)

Expression

Insect

Mutation

Partial deletion of Gr66a (see associated paper l…

Available Since

May 18, 2011

Availability

Academic Institutions and Nonprofits only
ninaE-stops m3-myc

Plasmid

#26855

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

Slow Termination of Phototransduction (stops Fly)

Tags

myc

Expression

Insect

Mutation

Amino acids 296-299 (SLRH in wild-type) are mutat…

Available Since

April 5, 2011

Availability

Academic Institutions and Nonprofits only
pRmHa3-dKDM4A-H195A-HA2FL2

Plasmid

#20235

Depositor

Jerry Workman

Article

Lin et al Mol Cell. 2008 Dec 5. 32(5):696-706.

Insert

dKDM4A (Kdm4A Fly)

Tags

FLAG2 and HA2

Expression

Insect

Mutation

changed His 195 to Ala

Available Since

March 19, 2009

Availability

Academic Institutions and Nonprofits only
CJ38 (miR-1006 seed mutant perfect site)

Plasmid

#17771

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

miR-1006 seed mutant perfect site

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ39 (miR-1003 seed mutant perfect site)

Plasmid

#17772

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

miR-1003 seed mutant perfect site

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

seed mutant

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ45 (miR-1003 5' splice site mut)

Plasmid

#17775

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

dme-miR-1003 minigene 5' splice mut

Use

Drosophila metallothionein gene promoter drives e…

Expression

Insect

Mutation

5' splice site mut + compsensatory stem

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ50 (cg11094 utr mut)

Plasmid

#17778

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg11094 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
CJ58 (cg6551 utr mut)

Plasmid

#17784

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg6551 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pMT Draper-delta ITAM-GFP

Plasmid

#126655

Purpose

Inducible expression of Draper-delta ITAM-GFP mutant in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Draper with ITAM Tyr residues mutated to Ala

Expression

Insect

Mutation

Cytoplasmic ITAM Tyr residues mutated to Phe

Availability

Academic Institutions and Nonprofits only

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pMT FRB-Draper INT-GFP

Plasmid

#126665

Purpose

Inducible expression of a synthetic FRB-Draper receptor in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

FRB-Draper INT domain

Expression

Insect

Mutation

K986N- see depositor comments below

Availability

Academic Institutions and Nonprofits only

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pCFD4-U6:1_U6:3tandemgRNAs

Plasmid

#49411

Purpose

expression of two gRNAs from Drosophila U6:1 and U6:3

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Inserts

dU6-1:gRNA
dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1 and dU6-3

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFasBac+GFP-CAMSAP2

Plasmid

#59037

Purpose

for baculovirus expression of CAMSAP2 in Sf9 cells

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

CAMSAP2 (CAMSAP2 Human)

Tags

His and eGFP

Expression

Insect

Available Since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pLp_3050sNuc

Plasmid

#122030

Purpose

Plasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakei

Depositor

Vincent Eijsink, Geir Mathiesen

Article

Mathiesen et al BMC Genomics. 2009 Sep 10;10:425. doi: 1

Insert

signal peptide Lp_3050

Expression

Bacterial

Promoter

sppA

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p2R3z-BASI-ccdB-BSAI

Plasmid

#118389

Purpose

Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

ccdB

Use

Unspecified

Mutation

See Depositor Comments Section

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHACK-GAL4>QF2(newHA1)

Plasmid

#104873

Purpose

HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

T2A-QF2

Use

CRISPR

Expression

Insect

Available Since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFD3-dU6:3gRNA

Plasmid

#49410

Purpose

expression of gRNA under control of the Drosophila U6:3 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-3:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-3

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
MD-2

Plasmid

#20864

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Myeloid differentiation factor 2 (LY96 Human)

Tags

protein A tag

Expression

Insect

Mutation

contains amino acids 19–160

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPnlsLexA::p65Uw

Plasmid

#26230

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

nlsLexA::p65 (RELA Human)

Expression

Insect

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacPak-HA-E(y)2

Plasmid

#52288

Purpose

Expresses Drosophila E(y)2

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

E(y)2 (e(y)2 Fly)

Tags

HA

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPp65ADZpUw

Plasmid

#26234

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

p65AD-Zip (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-BbsI-chiRNA

Plasmid

#45946

Purpose

Plasmid for expression of chiRNA under the control of the Drosophila snRNA:U6:96Ab promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2013 May 24.

Insert

U6-BbsI-chiRNA

Use

CRISPR

Expression

Insect

Promoter

Dm-snRNA:U6:96Ab

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSupAR-MbPylRS(DiZPK)

Plasmid

#91705

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

Depositor

P. Chen

Article

Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

Inserts

pylS
pylT

Expression

Bacterial

Mutation

L274A, C313S

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBPLexA::p65Uw

Plasmid

#26231

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

LexA::p65 (RELA Human)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
HPRT

Plasmid

#22884

Depositor

James Thomson

Article

Zwaka et al Nat Biotechnol. 2003 Mar . 21(3):319-21.

Insert

hypoxanthine phosphoribosyltransferase-1 (HPRT1 Human)

Use

Cre/Lox; Homologous recombination

Mutation

PGK, NeoR, and BGH polyA are between the two geno…

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
psPAX2-D64V-NC-COM

Plasmid

#131226

Purpose

Expressing GAG precursor protein, in which COM is inserted after the second zinc finger domain of nucleocapsid protein (NC)

Depositor

Baisong Lu

Article

Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

Insert

COM

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1_hMLH1

Plasmid

#129426

Purpose

Expression human MLH1 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MLH1 (MLH1 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac{3XP3::EYFP,attP}

Plasmid

#86860

Purpose

Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

attP

Expression

Insect

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
CMVTO-hIL2

Plasmid

#233387

Purpose

secreted human IL-2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL-2 (IL2 Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pACU2_CD4-mIFP T2A HO1

Plasmid

#72441

Purpose

Expressing UAS-CD4-mIFP T2A HO1 in drosophila

Depositor

Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

CD4-mIFP T2A HO1

Expression

Insect

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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We narrowed to 10,521 results for: SEC