We narrowed to 9,644 results for: SUB

1. pcDNA3-HA-APC11

   Plasmid

   #19900

   Depositor

   Yue Xiong

   Article

   Zacharek et al Cancer Res. 2005 Dec 15. 65(24):11354-60

   Insert

   APC11 (Anaphase promoting complex subunit 11) (ANAPC11 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

2. CFP-tTA-ER-C3

   Plasmid

   #134569

   Purpose

   Expresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

   Depositor

   Susan Janicki

   Article

   Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

   Insert

   tTA-ER
   

   Tags

   CFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMito-iRFP720

   Plasmid

   #45464

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP720
   

   Tags

   human cytochrome C oxidase subunit VIII

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pECFP(C1)-PP1gamma

   Plasmid

   #44232

   Depositor

   Laura Trinkle-Mulcahy, Angus Lamond

   Article

   Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

   Insert

   PP-1G (PPP1CC Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

5. T. aq RNA Polymerase

   Plasmid

   #215349

   Purpose

   Express T. aq RNA Polymerase subunits in E. coli cells

   Depositor

   Julius Lucks

   Article

   Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

   Insert

   RNA polymerase
   

   Tags

   6x His

   Expression

   Bacterial

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMKO.1-Puro-shPP2A-Aalpha

   Plasmid

   #15249

   Depositor

   William Hahn

   Article

   Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

   Insert

   PP2A regulatory subunit A, alpha isoform (PPP2R1A Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   July 12, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-C3-Rabaptin-5

   Plasmid

   #199173

   Purpose

   Expresses GFP-Rabaptin-5 fusion in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

   Insert

   Rabaptin-5 (RABEP1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains Tyr 149 Ser substitution; insert starts …

   Promoter

   CMV

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YFP-Rac2(V12)

   Plasmid

   #11397

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Obtained with Glycine 12 changed to Valine.

   Available Since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-p40R57Q PX

   Plasmid

   #19009

   Depositor

   Michael Yaffe

   Article

   Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

   Insert

   p40phox (NCF4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa1-148 (PX domains) site directed mutagenesis: …

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

10. C1QBP

    Plasmid

    #32863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    C1QBP (C1QBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids E89-Q282 only

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pECFP(C1)-PP1alpha

    Plasmid

    #44233

    Depositor

    Laura Trinkle-Mulcahy, Angus Lamond

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP1A (PPP1CA Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

12. GPKA

    Plasmid

    #61091

    Purpose

    GFP-tagged cAMP-dependent PKA catalytic subunit

    Depositor

    Steven Green

    Article

    Bok et al J Neurosci. 2003 Feb 1;23(3):777-87.

    Insert

    pkac2
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YFP-Rac2(N17)

    Plasmid

    #11398

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 changed to Asparagine.

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGGF012

    Plasmid

    #48849

    Purpose

    Provides a sulfadiazine resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pMAS::SulfadiazineR::t35S
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition sites in SulfR removed …

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDM_noro_A2_lacZA

    Plasmid

    #118819

    Purpose

    T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch A2
    

    Expression

    Bacterial

    Available Since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVU-tTR-KRAB

    Plasmid

    #11645

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    hUbiquitin C, GFP, tTR-KRAB, no tetO
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGGE001

    Plasmid

    #48839

    Purpose

    Provides the rbcS terminator as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    rbcS terminator
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJPF0190

    Plasmid

    #130268

    Purpose

    Piggybac vector for the expression of mRuby2-fused human PRKACA

    Depositor

    Hana El-Samad

    Article

    Fonseca et al bioRxiv 2020, 747030

    Insert

    PRKACA (PRKACA Human)
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CMVTO-IL12-26Sfur-B2AD-tvmvs-KKMP

    Plasmid

    #179646

    Purpose

    TVMVP-inducible IL-12 RELEASE construct

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    IL12 A/B
    

    Expression

    Mammalian

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMito-iRFP713

    Plasmid

    #45465

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP713
    

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCellFree_G03 POLD1

    Plasmid

    #67128

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pY3

    Plasmid

    #218116

    Purpose

    Activity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p18S.7 tag

    Plasmid

    #51730

    Purpose

    transcribes truncated transcript containing 18S and hybridization tag

    Depositor

    Vincent Mauro

    Article

    Burman et al Nucleic Acids Res. 2012 Sep;40(16):8085-

    Insert

    18S
    

    Promoter

    pol-I

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMKO.1-Puro-shPP2A-Abeta

    Plasmid

    #15250

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    PP2A regulatory subunit A, beta isoform (PPP2R1B Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pEYFP(C1)-PP1gamma

    Plasmid

    #44230

    Depositor

    Laura Trinkle-Mulcahy, Angus Lamond

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP-1G (PPP1CC Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

26. RAC1 (RAC1A-c007)

    Plasmid

    #110257

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    StrepII-TEV

    Expression

    Bacterial

    Available Since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQFT-lacZ

    Plasmid

    #208896

    Purpose

    pQFT with lacZ under control of PQJ

    Depositor

    Urs Jenal

    Article

    Klotz et al Appl Environ Microbiol. 2023 May 18:e002

    Insert

    lacZ
    

    Expression

    Bacterial

    Mutation

    Contains two silent mutations and a missense muta…

    Promoter

    PQJ

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pL-rpb1-N488D

    Plasmid

    #10988

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    rpb1-N488D (RPO21 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Mutation 1462 A to G in the RPB1 ORF resulting in…

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

29. ABCB8 (ABCB8A-c016)

    Plasmid

    #110266

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ABCB8 (ABCB8 Human)
    

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. cacna2d1 R241A D491A HA pcDNA3

    Plasmid

    #206109

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding

    Depositor

    Annette Dolphin

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Expression

    Mammalian

    Mutation

    R241A D491A

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGGF002

    Plasmid

    #48843

    Purpose

    Provides a BASTA resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    p35S::BastaR::t35S
    

    Use

    Golden gate compatible cloning vector

    Mutation

    internal BsaI recognition site in 35S promoter re…

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PKC beta II dNPS

    Plasmid

    #16383

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC beta II (Prkcb Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N-terminal pseudosubstrate domain deletion: aa 30…

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pDM_noro_S2_lacZA

    Plasmid

    #118816

    Purpose

    T7 RNAP-driven expression of norovirus sense orientation toehold switches (S2) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch S2
    

    Expression

    Bacterial

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSK ZO1

    Plasmid

    #30316

    Depositor

    James Anderson, Alan Fanning

    Article

    Willott et al Proc Natl Acad Sci U S A. 1993 Aug 15. 9

    Insert

    Zonula Occludens-1 (TJP1 Human)
    

    Use

    Subcloning

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pLpC-L-IFP-F[1] (mammalian)

    Plasmid

    #52901

    Purpose

    Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[1]
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pLpC-L-IFP-F[2] (mammalian)

    Plasmid

    #52902

    Purpose

    Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    L-IFP-F[2]
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Unknown

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pRK1036

    Plasmid

    #17728

    Depositor

    David Waugh

    Article

    Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

    Insert

    NusG (nusG Aquifex aeolicus)
    

    Tags

    His6 and MBP

    Expression

    Bacterial

    Available Since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pETM6_Pfrm_BsMdh_BmHps_BmPhi

    Plasmid

    #129109

    Purpose

    Expresses Bacillus subtilis Mdh and Bacillus methanolicus Hps & Phi under Pfrm promoter

    Depositor

    Eleftherios Terry Papoutsakis

    Article

    Rohlhill et al ACS Synth Biol. 2017 Aug 18;6(8):1584-15

    Insert

    BsMDH BmHPS BmPHI
    

    Expression

    Bacterial

    Available Since

    Nov. 11, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pDM_noro_S6_lacZA

    Plasmid

    #118817

    Purpose

    T7 RNAP-driven expression of norovirus sense orientation toehold switches (S6) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch S6
    

    Expression

    Bacterial

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. CYP7A1 (3SN5)

    Plasmid

    #32874

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP7A1 (CYP7A1 Human)
    

    Expression

    Bacterial

    Mutation

    amino acids 25-503 only

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits