We narrowed to 6,653 results for: SIM;

1. pQTEV-PRKRA

   Plasmid

   #31571

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ2370 - intron GG3 - 250bp

   Plasmid

   #159879

   Purpose

   Golden Gate compatible intron with PATCs for reduced germline silencing

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   intron GG3 - 250bp
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORE303N_CEN12RRFuzzy3MYB

   Plasmid

   #194441

   Purpose

   CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

   Depositor

   Chung-Jui Tsai

   Article

   Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

   Insert

   gRNA array targeting CEN1, RR, Fuzzy3MYB
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   MtU6

   Available since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MTK234_033

   Plasmid

   #123996

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 2) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA2 -hAAVS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MTK234_034

   Plasmid

   #123997

   Purpose

   Encodes the spCas9 sgRNA1 hAAVS1 (site 3) gRNA expression cassette as a type 234 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   sgRNA3 -hAAVS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1α-mouse-retroCHMP3-HA

   Plasmid

   #154178

   Purpose

   expresses mouse retroCHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

   Insert

   retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MTK0_031

   Plasmid

   #123970

   Purpose

   Encodes the BxBI-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   BxBI-hygro-mRuby2PEST landing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRN3P_T3_BE3_IVT

   Plasmid

   #177015

   Purpose

   Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

   Insert

   BE3
   

   Use

   CRISPR; Vector for in-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T3 promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-optoB2-LYY-YFP

   Plasmid

   #75271

   Purpose

   G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

   Depositor

   Michael Bruchas

   Article

   Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

   Insert

   G-protein independent version of optoB2
   

   Use

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   point mutations at L72F,Y136G,Y224A

   Promoter

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEN_TTRmiRc2

    Plasmid

    #25754

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    dsRed2

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA-NLS-ZapCmR2

    Plasmid

    #59012

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR2 genetically encoded Zn(II) sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pEN_TGmiRc3

    Plasmid

    #25749

    Purpose

    Entry vector for cloning miR30-based shRNA driven by TRE promoter with GFP coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. PARP15-c021

    Plasmid

    #173102

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    G287-N470, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK_ToeRepG2_N02_trigger

    Plasmid

    #132745

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 2 trigger RNA

    Depositor

    Peng Yin, Alexander Green

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N02_trigger
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MTK7_013

    Plasmid

    #123880

    Purpose

    Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    RA_hAAVS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Plas-gRNA-LEU2

    Plasmid

    #157656

    Purpose

    GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    GFP-gRNA
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    GFP-gRNA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MTK1_008

    Plasmid

    #123671

    Purpose

    Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS-2xHS4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPHAGE2-Myc-mTZAP

    Plasmid

    #87188

    Purpose

    lentiviral stable expression of Myc-TZAP (mouse) in mouse embryonic stem cells

    Depositor

    Eros Lazzerini Denchi

    Article

    Li et al Science. 2017 Jan 12. pii: aah6752. doi:

    Insert

    TZAP (Zbtb48 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    unknown

    Available since

    March 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MTK0_030

    Plasmid

    #123969

    Purpose

    Encodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    phiC31-hygro-mRuby2PEST landing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge