159,461 results

1. pLentiPGK DEST H2B-iRFP670

   Plasmid

   #90237

   Purpose

   Lentiviral vector to express H2B-iRFP670 under PGK promoter

   Depositor

   Markus Covert

   Article

   Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

   Insert

   H2B
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   March 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PA-mCherry1-N1

   Plasmid

   #54507

   Purpose

   Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pPEgRNA

   Plasmid

   #172716

   Purpose

   Delivery of guide RNA for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-MBP-super TEV protease

   Plasmid

   #171782

   Purpose

   Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

   Depositor

   Mark Howarth

   Article

   Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

   Insert

   MBP-sTEV
   

   Use

   Tags

   Arg6 tag and Internal His6 tag

   Expression

   Bacterial

   Mutation

   No internal TEV cleavage site between the MBP and…

   Promoter

   T7

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AiP13572 - pAAV-AiE0390h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3572)

   Plasmid

   #208143

   Purpose

   AiE0390h_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Mich et al bioRxiv 2023.09.20.558718

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRC0569 (parts entry vector)

   Plasmid

   #189966

   Purpose

   Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BBa-J23100

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV10-mFoxP1

   Plasmid

   #35170

   Depositor

   Benjamin Blencowe

   Article

   Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

   Insert

   Foxp1 (Foxp1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

8. Tol2-mpx:Lifeact-Ruby

   Plasmid

   #45246

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

   Insert

   lifeact-ruby
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   mpx

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-Basic-IRES

   Plasmid

   #64784

   Purpose

   Luciferase reporter vector

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-EIN-FTO(32-505)

    Plasmid

    #186466

    Purpose

    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

    Depositor

    Vincenzo Venditti

    Article

    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

    Insert

    Fat mass obesity associated protein (FTO Human)
    

    Use

    Tags

    6 Histidine-EIN (N-terminal domain of E.coli Enzy…

    Expression

    Bacterial

    Mutation

    amino acid 32-505

    Promoter

    T7

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-mGreenLantern

    Plasmid

    #161912

    Purpose

    mGreenLantern fluorescent protein: cytosolic expression in mammalian cells

    Depositor

    Gregory Petsko

    Article

    Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    mGreenLantern
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-Sec23A

    Plasmid

    #66609

    Purpose

    GFP fusion of Sec23A

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec23A (SEC23A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-Clover-HIF-1alpha Wildtype

    Plasmid

    #163365

    Purpose

    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV Promoter

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMitoLOC

    Plasmid

    #58980

    Purpose

    Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    preCOX4 and preSU9

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and MET17

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-PGK-Neo-PIP-FUCCI

    Plasmid

    #118616

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFB2G

    Plasmid

    #195290

    Purpose

    Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A mTagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

    Plasmid

    #98927

    Purpose

    Cre dependent AAV expression of GFP spaghetti monster myc construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1

    Insert

    GFP-smFP-myc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAM2991

    Plasmid

    #40248

    Depositor

    Susan Golden

    Article

    Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

    Insert

    One Step Cloneing Vector for Overexpression
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. HS-GFP-PACT

    Plasmid

    #105954

    Purpose

    heat shock inducible transgenesis, PACT is a centriol marker

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    PACT (AKAP9 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP12315 - pAAV-AiE0464m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2315)

    Plasmid

    #223981

    Purpose

    AiE0464m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    minBG

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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