164,577 results

1. pAAV-R26-FLEX-mStr

   Plasmid

   #135619

   Purpose

   Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

   Depositor

   Lin He

   Article

   Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

   Insert

   CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLBH531_MBP-Cas14a1 expression

   Plasmid

   #112500

   Purpose

   Expresses MBP fused to Cas14a1

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT_mEGFP-SNAP-tag2

   Plasmid

   #226513

   Purpose

   Mamalian cell expression of cytosolic mEGFP-SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   mEGFP-SNAP-tag2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p-eGFP-beta 2 CP

   Plasmid

   #13298

   Depositor

   John Cooper

   Article

   Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

   Insert

   capping protein beta 2 subunit (Capzb Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE reporter backbone

   Plasmid

   #158678

   Purpose

   This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ppyCAG_RNaseH1_WKKD

   Plasmid

   #111905

   Purpose

   Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…

   Promoter

   Available Since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCG_IFITM1

   Plasmid

   #179955

   Purpose

   Expression vector for human IFITM1.

   Depositor

   Konstantin Sparrer, Frank Kirchhoff

   Article

   Prelli Bozzo et al Nat Commun. 2021 Jul 28;12(1):4584.

   Insert

   IFITM1 (IFITM1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P13A

   Promoter

   Available Since

   Feb. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWZL Blast DN E-cadherin

   Plasmid

   #18800

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   Dom neg E-cadherin (Cdh1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   truncated form of E-cadherin (dominant negative) …

   Promoter

   Available Since

   July 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1 Full length Importin beta

   Plasmid

   #106941

   Purpose

   Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)

   Depositor

   Patrizia Lavia

   Article

   Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511

   Insert

   human Importin beta-1 (KPNB1) (KPNB1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYC-N

    Plasmid

    #207113

    Purpose

    HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

    Depositor

    Yifan Cheng

    Article

    Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT/Caggs-NRASV12

    Plasmid

    #20205

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    NRas (NRAS Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available Since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-EGFP-CNA35

    Plasmid

    #61603

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with EGFP

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    EGFP and CNA35
    

    Use

    Tags

    His tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer20 ALPK1 Y254C

    Plasmid

    #231587

    Purpose

    ALPK1 gene mutated in position Y254C (Pathogenic mutation in ROSAH syndrome) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed tyrosine 254 to cysteine

    Promoter

    doxycycline

    Available Since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3X ERE TATA luc

    Plasmid

    #11354

    Purpose

    Luciferase reporter containing three copies of vitellogenin Estrogen Response Element

    Depositor

    Donald McDonnell

    Article

    Hall et al Endocrinology. 1999 Dec . 140(12):5566-7

    Insert

    ERE
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. T7-TPase

    Plasmid

    #51818

    Purpose

    For in vitro transcription of Tol2 transposase using T7 Promoter

    Depositor

    Elly Tanaka

    Article

    Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

    Insert

    Tol2 Transposase
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    April 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Christian Mosimann, Alexa Burger

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. lenti F9-TetR-EGFP-IRES-PuroR

    Plasmid

    #117049

    Purpose

    lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Insert

    F9-TetR-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    F9 promoter

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SpRY-ABE8e

    Plasmid

    #185671

    Purpose

    Expresses SpRY-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY D10A

    Promoter

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS415-GPD

    Plasmid

    #183940

    Purpose

    Control vector that has a GPD promoter with no insert

    Depositor

    Michael Downey

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

    Plasmid

    #40810

    Depositor

    Natalie Ahn

    Article

    Mansour et al Science. 1994 Aug 12;265(5174):966-70.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(31-51), S218E, S222D

    Promoter

    CMV

    Available Since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only